FastQCFastQC Report
Fri 27 May 2016
SRR940032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277522
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38270.29956431278678564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.2307592354573933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.13870602619759193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.067.437531
GTATCAA43100.058.401391
ATCAACG55000.045.3209843
TCAACGC55550.044.872264
CAACGCA56700.043.8829655
TATCAAC57600.043.2769432
AACGCAG59500.041.8047836
ACGCAGA68200.036.2888877
CGCAGAG69300.035.7128758
GTACATG63600.035.4505271
TACATGG64150.034.674882
ACATGGG66300.033.0993963
GTGGTAT8900.031.4989321
GCAGAGT78100.031.4603619
CATGGGG37150.028.4118964
GTATAGG4000.028.3322851
CATGGGA32850.027.9557484
GAGTACT46350.027.0830412-13
GTACTTT47300.026.3504214-15
CAGAGTA78750.025.30806410-11
Produced by FastQC (version 0.11.5)