FastQCFastQC Report
Fri 27 May 2016
SRR940031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170816
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12390.10582363069858969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.054.1571961
GTATCAA21000.047.4544071
TCAACGC23750.040.4348144
ATCAACG24000.040.008483
CAACGCA24550.039.0836875
AACGCAG26250.036.7795876
GTACATG56450.034.672831
TATCAAC28550.034.2840042
TACATGG58200.032.8156362
ACATGGG59050.031.309723
ACGCAGA32000.029.9845147
CGCAGAG32100.029.8911068
ACGATAG800.00531990429.7982775
TAGACCG1159.953603E-425.9115475
CATGGGA34550.025.723584
GAGTACA41400.025.6565951
TCGCAAC600.001291506524.780966114-115
CATGGGG26150.024.1783894
AGTACAT40950.023.9025252
ACCGCGA1001.8333903E-623.790745112-113
Produced by FastQC (version 0.11.5)