FastQCFastQC Report
Fri 27 May 2016
SRR940031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170816
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.3988671149010604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.29227478954848585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.18875724281185088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.069.611671
GTATCAA44550.061.4752431
ATCAACG56000.048.038963
TCAACGC56500.047.6138344
CAACGCA57650.046.679565
TATCAAC58500.046.0899542
AACGCAG60700.044.21146
ACGCAGA69550.038.579067
CGCAGAG70650.037.809988
TGGTATC10650.033.6066552
GCAGAGT79100.033.4700059
GTACATG56600.032.5744671
GTGGTAT11300.032.2096981
TACATGG58400.031.2559192
ACATGGG59450.029.5988753
AGAGTAC74400.026.868210-11
AGTACTT52900.026.20429412-13
GAGTACT50200.026.19152512-13
CAGAGTA77200.026.00930210-11
GAGTACA43200.025.5519031
Produced by FastQC (version 0.11.5)