FastQCFastQC Report
Fri 27 May 2016
SRR940030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291357
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.048.4970471
GGTATCA16350.045.977261
ATCAACG28400.041.1345183
TCAACGC28550.041.130364
CAACGCA29600.039.6497965
AACGCAG31350.037.2464566
GTACATG66100.035.9229431
TATCAAC33600.035.1518942
TACATGG67800.034.576872
ACATGGG70750.032.434183
ACGCAGA38300.030.1765377
CGCAGAG39550.028.770898
CATGGGG34450.027.5112464
CATGGGA39050.026.407514
GGACCGA3201.8189894E-1124.2024776
AGTACAT48800.023.8362312
GCAGAGT46250.023.6921279
ACCGTAT902.1763688E-523.14169712-13
GAGTACA52550.022.8198951
ATGGGGA20700.021.2974595
Produced by FastQC (version 0.11.5)