FastQCFastQC Report
Fri 27 May 2016
SRR940030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291357
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.30549259422452507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.22286633363198557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.13598098744189252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.066.78231
GTATCAA47600.057.889291
ATCAACG59100.046.4086233
TCAACGC60050.045.674434
CAACGCA60700.045.1135525
TATCAAC61900.044.311082
AACGCAG62050.044.30166
ACGCAGA73650.037.077357
CGCAGAG75550.036.1448948
GTGGTAT10750.034.953871
GTACATG64200.033.909471
TACATGG66800.032.937842
GCAGAGT83500.032.418569
GTATAGG3500.030.6738031
ACATGGG69400.030.41393
TGGTATC13750.027.3201482
GAGTACT49850.026.31553612-13
CAGAGTA82250.026.29282610-11
CATGGGG34000.025.7790414
GTATTAG4950.025.3033071
Produced by FastQC (version 0.11.5)