FastQCFastQC Report
Fri 27 May 2016
SRR940029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524364
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.047.9116941
GTATCAA20400.042.118451
CAACGCA21150.039.43675
ATCAACG21200.039.369573
TCAACGC21150.039.185924
AACGCAG23500.035.746556
TATCAAC24450.034.164492
GTACATG50400.030.3074571
ACGCAGA28000.029.3632417
TACATGG49100.029.2860852
CGCAGAG29700.027.6825148
ACATGGG51900.026.7643743
CATGGGG35900.025.5771374
GTATAGG7000.022.1623271
GAGTACA40250.020.9022621
GCAGAGT38400.020.471729
AGTACAT38050.020.0715562
TAGCCCT11950.019.958034
CAGAGTA35550.019.07996210-11
TACACTG17050.018.8691485
Produced by FastQC (version 0.11.5)