FastQCFastQC Report
Fri 27 May 2016
SRR940029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524364
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.13290788814220225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.10332177878774361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.062.88761
GTATCAA34700.053.0950781
ATCAACG40250.044.430453
CAACGCA40350.044.2534375
TCAACGC40450.044.0633964
AACGCAG42950.041.7007986
TATCAAC43600.041.5648962
ACGCAGA52150.033.7692537
CGCAGAG54300.032.3225948
GTACATG50050.029.544241
TACATGG48950.028.253672
GCAGAGT64350.026.8122639
ACATGGG51800.025.8927463
GAGTACT34450.024.52353912-13
TAACGCA1501.6657881E-423.8443414
CAGAGTA60150.023.78830510-11
CATGGGG36100.023.778294
TGGTATC10700.023.3994222
TTCGCGC650.002053054522.88438248-49
GTGTAGC12650.022.6243421
Produced by FastQC (version 0.11.5)