FastQCFastQC Report
Fri 27 May 2016
SRR940028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025622
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25290.2465820740974745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21050.046.2033921
GGTATCA13200.042.4905431
ATCAACG23300.039.140643
TCAACGC23900.038.4074334
CAACGCA24100.038.06085
GTACATG50550.035.7651331
TATCAAC27300.035.4036672
AACGCAG25650.035.2964026
TACATGG49850.034.8276142
ACATGGG54800.030.020713
ACGCAGA30250.029.9290147
CGCAGAG30850.028.9607838
CATGGGG18650.026.8468234
ACTACGC1159.985843E-425.8968168
CATGGGA37350.025.055414
GCAGAGT36800.023.6239249
GGACCGA2552.4503606E-823.3579146
GTAAGGT4650.023.0734214
TAGCCCT9350.022.9500354
ACGCGCA700.003154198221.25266612-13
Produced by FastQC (version 0.11.5)