FastQCFastQC Report
Fri 27 May 2016
SRR940028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025622
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80300.7829395235281614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.5119819972660493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.3777220067432251No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.1194397156067245No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.11924471198940739No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.065.016871
GTATCAA54250.056.1382261
ATCAACG66900.044.8929673
TCAACGC67850.044.0887454
CAACGCA68600.043.6403165
TATCAAC69200.043.4869732
AACGCAG70750.042.217676
TAACGCA1455.820766E-1141.0965964
TATAACG1603.6379788E-1240.968172
ATAACGC1707.2759576E-1238.558283
ACGCAGA80200.037.165337
CGCAGAG81550.036.477138
TATCACG1001.0511221E-535.754042
ATCACGC1001.0511221E-535.754043
GTGGTAT12650.034.3929821
TGGTATC13050.033.3339042
GTACATG51800.033.0209541
TACATGG51750.032.932872
GCAGAGT90750.032.7791759
ACATGGG51700.031.1205393
Produced by FastQC (version 0.11.5)