##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940028_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1025622 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.144569831770376 34.0 33.0 34.0 31.0 34.0 2 33.18764125574529 34.0 33.0 34.0 31.0 34.0 3 33.254970154696366 34.0 34.0 34.0 31.0 34.0 4 36.47602333023278 37.0 37.0 37.0 35.0 37.0 5 36.48945420437549 37.0 37.0 37.0 35.0 37.0 6 36.570207152342675 37.0 37.0 37.0 35.0 37.0 7 36.55877409025938 37.0 37.0 37.0 35.0 37.0 8 36.5627190134377 37.0 37.0 37.0 35.0 37.0 9 38.440266491943426 39.0 39.0 39.0 37.0 39.0 10-11 38.42579771104754 39.0 39.0 39.0 37.0 39.0 12-13 38.4142144961789 39.0 39.0 39.0 37.0 39.0 14-15 40.01960225112176 41.0 40.0 41.0 38.0 41.0 16-17 40.02811708407191 41.0 40.0 41.0 38.0 41.0 18-19 40.08094746407546 41.0 40.0 41.0 38.0 41.0 20-21 40.079334784160245 41.0 40.0 41.0 38.0 41.0 22-23 40.02605979590922 41.0 40.0 41.0 38.0 41.0 24-25 39.993662869946235 41.0 40.0 41.0 38.0 41.0 26-27 39.89597580785124 41.0 40.0 41.0 38.0 41.0 28-29 39.80834508230127 41.0 40.0 41.0 37.0 41.0 30-31 39.6367550618064 41.0 40.0 41.0 36.5 41.0 32-33 39.42874031563285 41.0 40.0 41.0 35.0 41.0 34-35 39.306858667228276 41.0 40.0 41.0 35.0 41.0 36-37 39.19858924633052 41.0 39.5 41.0 35.0 41.0 38-39 39.07281240067003 41.0 39.0 41.0 35.0 41.0 40-41 38.93500139427586 41.0 39.0 41.0 35.0 41.0 42-43 38.759954447154996 41.0 39.0 41.0 35.0 41.0 44-45 38.514533132089596 41.0 39.0 41.0 35.0 41.0 46-47 38.16690505858884 41.0 39.0 41.0 34.5 41.0 48-49 37.84581941495015 41.0 38.0 41.0 34.0 41.0 50-51 37.84203731979228 41.0 39.0 41.0 34.0 41.0 52-53 37.75276807634781 41.0 38.5 41.0 34.0 41.0 54-55 37.61286711868505 41.0 38.0 41.0 34.0 41.0 56-57 37.45057487066385 41.0 37.5 41.0 34.0 41.0 58-59 37.25684072689548 40.5 37.0 41.0 33.0 41.0 60-61 37.04021803354452 40.0 36.5 41.0 33.0 41.0 62-63 36.791271540587076 39.5 36.0 41.0 33.0 41.0 64-65 36.50151469059751 39.0 35.5 41.0 32.5 41.0 66-67 36.18752230353873 39.0 35.0 41.0 32.0 41.0 68-69 35.8407931967138 38.0 35.0 41.0 32.0 41.0 70-71 35.473781276142674 37.0 35.0 40.0 31.0 41.0 72-73 35.10106744980119 37.0 35.0 39.0 31.0 41.0 74-75 34.725426619163784 36.0 35.0 39.0 31.0 41.0 76-77 34.35015727041737 36.0 35.0 38.5 31.0 40.5 78-79 34.00263644890613 35.0 35.0 37.0 30.5 39.0 80-81 33.68007511539339 35.0 35.0 37.0 30.0 39.0 82-83 33.381964797947006 35.0 35.0 36.5 30.0 38.5 84-85 33.12123277386796 35.0 35.0 36.0 30.0 37.0 86-87 32.90537254466071 35.0 35.0 36.0 29.0 37.0 88-89 32.786294073255064 35.0 35.0 35.0 29.5 36.5 90-91 32.76278589967844 35.0 35.0 35.0 30.0 36.0 92-93 32.6686196278941 35.0 35.0 35.0 30.0 36.0 94-95 32.583179767984696 35.0 35.0 35.0 30.0 36.0 96-97 32.51098357874538 35.0 35.0 35.0 30.0 36.0 98-99 32.4564917679223 35.0 35.0 35.0 30.0 36.0 100-101 32.39742224718268 35.0 35.0 35.0 29.0 35.0 102-103 31.938312068188864 35.0 33.5 35.0 28.0 35.0 104-105 32.30170130905928 35.0 34.5 35.0 29.0 35.0 106-107 32.34203341971994 35.0 35.0 35.0 29.0 35.0 108-109 32.323843482296596 35.0 35.0 35.0 29.0 35.0 110-111 32.2848442213603 35.0 35.0 35.0 29.0 35.0 112-113 32.24763314359481 35.0 35.0 35.0 29.0 35.0 114-115 32.19035083100792 35.0 35.0 35.0 29.0 35.0 116-117 32.13423902763397 35.0 35.0 35.0 29.0 35.0 118-119 32.07928993332827 35.0 35.0 35.0 29.0 35.0 120-121 32.00373675681684 35.0 35.0 35.0 29.0 35.0 122-123 31.901193617141598 35.0 35.0 35.0 29.0 35.0 124-125 30.894422604039306 34.5 33.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13862671626371537 1101 2 0.16263084550049456 1101 3 0.1282877239262561 1101 4 0.024966561803822174 1101 5 0.08944737233676392 1101 6 0.13057432799097768 1101 7 0.09070490048631541 1101 8 0.0972840245447344 1101 9 0.17709459320887788 1101 10-11 0.1468095252084609 1101 12-13 0.12348621633482537 1101 14-15 0.22012241093312923 1101 16-17 0.18117779952678603 1101 18-19 0.13394985566633721 1101 20-21 0.19105029393029582 1101 22-23 0.16070043965953573 1101 24-25 0.1624611363259163 1101 26-27 0.20043091592454942 1101 28-29 0.19767772517631244 1101 30-31 0.26458872822217216 1101 32-33 0.30935286593700084 1101 34-35 0.2580407464911971 1101 36-37 0.33918043178331914 1101 38-39 0.4184137925497211 1101 40-41 0.2943703950741394 1101 42-43 0.3074925448936625 1101 44-45 0.23728581883687383 1101 46-47 0.45547893859243516 1101 48-49 0.5256142023576444 1101 50-51 0.545050127941046 1101 52-53 0.42301566499794774 1101 54-55 0.4303966215917754 1101 56-57 0.3758496680507477 1101 58-59 0.46414697983048825 1101 60-61 0.4907640442634573 1101 62-63 0.5013388890045718 1101 64-65 0.4851187395246228 1101 66-67 0.44441280243741943 1101 68-69 0.4285443531503432 1101 70-71 0.4241094290637335 1101 72-73 0.45962773718068206 1101 74-75 0.33502874574779185 1101 76-77 0.44451679749720796 1101 78-79 0.44594139952759804 1101 80-81 0.4404369345151622 1101 82-83 0.4734488561179617 1101 84-85 0.47991077225007217 1101 86-87 0.4534095779752221 1101 88-89 0.42857692894458665 1101 90-91 0.4778757624244605 1101 92-93 0.43744699971048817 1101 94-95 0.41497751022125584 1101 96-97 0.43709917147064914 1101 98-99 0.4850745811598074 1101 100-101 0.513684498114479 1101 102-103 0.4857812652935962 1101 104-105 0.4506337013406352 1101 106-107 0.4933925377372148 1101 108-109 0.504591499942947 1101 110-111 0.48394619278795403 1101 112-113 0.5409334009496334 1101 114-115 0.613915528724668 1101 116-117 0.5822082096600738 1101 118-119 0.6175054305950809 1101 120-121 0.6104983479042119 1101 122-123 0.57147096501512 1101 124-125 0.419502048220064 1102 1 0.1741723473762491 1102 2 0.18312253902224285 1102 3 0.15970338961138708 1102 4 0.07825440196643996 1102 5 0.06642936678423439 1102 6 0.040056223604935326 1102 7 0.06272736566184989 1102 8 0.08346263646316743 1102 9 0.03179913202622231 1102 10-11 0.13089701135583454 1102 12-13 0.09609785675428384 1102 14-15 0.22317732283102742 1102 16-17 0.26986875925755527 1102 18-19 0.20547694178666376 1102 20-21 0.2085973865035058 1102 22-23 0.16217847885371128 1102 24-25 0.17917834623681728 1102 26-27 0.18985190459121526 1102 28-29 0.2133610090503808 1102 30-31 0.14022174090984407 1102 32-33 0.27889237829884905 1102 34-35 0.2724886114960938 1102 36-37 0.2684787648764768 1102 38-39 0.26143142827363164 1102 40-41 0.19367754029053685 1102 42-43 0.23762904991121303 1102 44-45 0.36287897930296253 1102 46-47 0.45449921157696593 1102 48-49 0.49890747517511613 1102 50-51 0.5105712610959259 1102 52-53 0.5227418642947654 1102 54-55 0.4833350374755341 1102 56-57 0.3634479755109581 1102 58-59 0.37656996939749376 1102 60-61 0.3706392462345178 1102 62-63 0.30457125487474457 1102 64-65 0.1823451524293489 1102 66-67 0.19080920515272481 1102 68-69 0.250685163483098 1102 70-71 0.11489157832910735 1102 72-73 0.10983235442719064 1102 74-75 0.09913547573592041 1102 76-77 0.18858806219629543 1102 78-79 0.3283355807206618 1102 80-81 0.2640082801939698 1102 82-83 0.2602270367397068 1102 84-85 0.3107658287637065 1102 86-87 0.4551350197610162 1102 88-89 0.4313952780551489 1102 90-91 0.34639244360796795 1102 92-93 0.3385258152897066 1102 94-95 0.4123657550273876 1102 96-97 0.45797854740398236 1102 98-99 0.40494483507903567 1102 100-101 0.35504970132254954 1102 102-103 0.40697110597398023 1102 104-105 0.36708080883451544 1102 106-107 0.3184282134459764 1102 108-109 0.32237088314532514 1102 110-111 0.4038193160671355 1102 112-113 0.44531708156053895 1102 114-115 0.403529912252246 1102 116-117 0.5036081512526565 1102 118-119 0.46517334235142016 1102 120-121 0.5315243162494312 1102 122-123 0.5076860496867504 1102 124-125 0.4498927147660048 1103 1 0.16951049102749494 1103 2 0.14453252420463514 1103 3 0.19178760647458404 1103 4 0.08362654902659727 1103 5 0.14819600233107622 1103 6 0.12070205589312621 1103 7 0.0699422307433224 1103 8 0.139687560631522 1103 9 0.20844310936054455 1103 10-11 0.15104791601292078 1103 12-13 0.21164398527224648 1103 14-15 0.26694004046079556 1103 16-17 0.2703419805024083 1103 18-19 0.22861400403407117 1103 20-21 0.20874972275426984 1103 22-23 0.24875563624704 1103 24-25 0.23626130892606056 1103 26-27 0.25224736460386765 1103 28-29 0.29718081912561445 1103 30-31 0.2620136557092181 1103 32-33 0.36642393148010655 1103 34-35 0.38894111011650523 1103 36-37 0.3947398214905036 1103 38-39 0.4887926707501222 1103 40-41 0.492231508313381 1103 42-43 0.4844611900458773 1103 44-45 0.6092935712804035 1103 46-47 0.6731210028799737 1103 48-49 0.7354373410335597 1103 50-51 0.626279514539398 1103 52-53 0.6650838781432924 1103 54-55 0.6535484672925733 1103 56-57 0.608116206734131 1103 58-59 0.6664924675651775 1103 60-61 0.6317032017645658 1103 62-63 0.6641212083173613 1103 64-65 0.6247235067328774 1103 66-67 0.5784150299036313 1103 68-69 0.6672596534625583 1103 70-71 0.7301859543683946 1103 72-73 0.7047519098745241 1103 74-75 0.6274944900653097 1103 76-77 0.6858340797351516 1103 78-79 0.6454075800941865 1103 80-81 0.7513625360038532 1103 82-83 0.8030448413602045 1103 84-85 0.731084180938204 1103 86-87 0.7014076759546128 1103 88-89 0.7862634919034264 1103 90-91 0.6837068105682533 1103 92-93 0.7041759484768662 1103 94-95 0.6752228734128494 1103 96-97 0.6119476714766634 1103 98-99 0.717117296845089 1103 100-101 0.7128318812848917 1103 102-103 0.6393545503883757 1103 104-105 0.6739799279761414 1103 106-107 0.6483974035922131 1103 108-109 0.627799253906069 1103 110-111 0.634515437671844 1103 112-113 0.7356280677572826 1103 114-115 0.7346694608230351 1103 116-117 0.6984492749435773 1103 118-119 0.649403848511561 1103 120-121 0.5996703167804682 1103 122-123 0.7130790588599574 1103 124-125 0.7803898815564558 1104 1 0.07291646040606992 1104 2 0.13524184828440866 1104 3 0.13458565020793145 1104 4 0.021739488308675448 1104 5 0.05622528515158365 1104 6 0.10991493632079141 1104 7 0.12709157288320228 1104 8 0.08974207131402778 1104 9 0.16837684003250075 1104 10-11 0.17760030805912663 1104 12-13 0.1887195208045256 1104 14-15 0.22945675768188778 1104 16-17 0.23101475611783684 1104 18-19 0.23020221651194106 1104 20-21 0.24077949011417132 1104 22-23 0.21123656362608756 1104 24-25 0.2576712818726108 1104 26-27 0.23655520129451446 1104 28-29 0.24436571862651846 1104 30-31 0.25913853839806933 1104 32-33 0.25024245679178136 1104 34-35 0.0907774654992366 1104 36-37 0.11463261103033062 1104 38-39 0.13270301383093397 1104 40-41 0.10851270512569755 1104 42-43 0.04571382228169796 1104 44-45 0.001811475378310945 1104 46-47 -0.02470016047954715 1104 48-49 0.0149985269804489 1104 50-51 0.00468429092324385 1104 52-53 0.03569319867466447 1104 54-55 -0.09672775687297275 1104 56-57 -0.09220351585482689 1104 58-59 -0.10184447330266266 1104 60-61 -0.10581287307474696 1104 62-63 -0.08867835266057256 1104 64-65 -0.08296097001963432 1104 66-67 -0.14200084194303741 1104 68-69 -0.1916225288245883 1104 70-71 -0.15944123171798452 1104 72-73 -0.11897455295114412 1104 74-75 -0.05824286021384495 1104 76-77 -0.078287918965394 1104 78-79 -0.12182140515061235 1104 80-81 -0.08371542467259019 1104 82-83 -0.05531456451618766 1104 84-85 -0.019296965584807424 1104 86-87 -0.0436875862044559 1104 88-89 -0.054083528852231666 1104 90-91 -0.10180221887240748 1104 92-93 -0.08926068392536024 1104 94-95 -0.09509107385800775 1104 96-97 -0.1570136033024454 1104 98-99 -0.14646803776240347 1104 100-101 -0.1343537523666143 1104 102-103 -0.08282481239336903 1104 104-105 -0.11015623983111311 1104 106-107 -0.12113222611445451 1104 108-109 -0.12700117336958527 1104 110-111 -0.15505038565971319 1104 112-113 -0.12375670179896048 1104 114-115 -0.0674277015625151 1104 116-117 -0.017584941369001683 1104 118-119 0.045628601378105316 1104 120-121 0.02014284058224547 1104 122-123 -0.04058710572926216 1104 124-125 -0.07012298382112547 1105 1 0.15027160853448152 1105 2 0.14775271007443536 1105 3 0.13409836990413737 1105 4 0.07420613481201599 1105 5 0.1327582977920656 1105 6 0.10053299017607031 1105 7 0.10883056346884956 1105 8 0.07570215753623444 1105 9 0.09029077144839448 1105 10-11 0.18025334527965953 1105 12-13 0.21921654671439228 1105 14-15 0.30774385939624693 1105 16-17 0.32114660984733945 1105 18-19 0.2971168440425558 1105 20-21 0.2742740100110481 1105 22-23 0.32864711288460313 1105 24-25 0.2952650773300931 1105 26-27 0.34308115677197293 1105 28-29 0.35423662535194467 1105 30-31 0.39300860018425254 1105 32-33 0.3551157399088396 1105 34-35 0.3522873623302374 1105 36-37 0.3655531627372426 1105 38-39 0.3711793147253175 1105 40-41 0.3083582396697935 1105 42-43 0.2546874611373795 1105 44-45 0.2959487492010382 1105 46-47 0.18829294078354053 1105 48-49 0.2742562526536432 1105 50-51 0.17425843674246977 1105 52-53 0.01673127374944272 1105 54-55 -0.018741904804549847 1105 56-57 0.0032218102389194314 1105 58-59 0.020852947232242514 1105 60-61 0.1402732819235979 1105 62-63 0.03888046903323783 1105 64-65 0.041071670559539086 1105 66-67 0.15450622242116907 1105 68-69 0.19073888433629094 1105 70-71 0.15115315423682318 1105 72-73 0.08775291573569888 1105 74-75 0.12874615557596059 1105 76-77 0.20386718019494765 1105 78-79 0.16199296745799074 1105 80-81 0.13177648839467793 1105 82-83 0.14599821182935813 1105 84-85 0.13684696693527343 1105 86-87 0.15632477583281457 1105 88-89 0.1169254534280526 1105 90-91 0.11234787306274541 1105 92-93 0.07331090787296546 1105 94-95 0.12913887923826195 1105 96-97 0.08807038653907995 1105 98-99 0.07760768991751377 1105 100-101 0.09754666482213992 1105 102-103 0.104476254822508 1105 104-105 0.16210742922154964 1105 106-107 0.06519308029604076 1105 108-109 0.10243343745428035 1105 110-111 0.12352932427648966 1105 112-113 0.1818058549103867 1105 114-115 0.16970977869423365 1105 116-117 0.20258534269380846 1105 118-119 0.11778149217450817 1105 120-121 0.1877564633072737 1105 122-123 0.235485391018198 1105 124-125 0.4100479483484989 1106 1 0.10450453224815703 1106 2 0.10013976132969304 1106 3 0.1287843244213036 1106 4 -0.006516238330597446 1106 5 0.03613086293687218 1106 6 -0.01539577689797511 1106 7 0.0718979473631407 1106 8 0.10427105025992489 1106 9 0.07720507488151895 1106 10-11 0.16120676143570734 1106 12-13 0.10714973129938699 1106 14-15 0.10139908008080312 1106 16-17 0.1868678145740219 1106 18-19 0.17960397461528288 1106 20-21 0.12512150477003559 1106 22-23 0.14742861236668148 1106 24-25 0.094653681230362 1106 26-27 0.08026284769450598 1106 28-29 0.17115764717660653 1106 30-31 0.1652062557127678 1106 32-33 0.154544227237146 1106 34-35 0.17325964166253272 1106 36-37 0.16776731090586594 1106 38-39 0.22920751174721232 1106 40-41 0.27849201205787466 1106 42-43 0.28903327180456984 1106 44-45 0.3190013706095698 1106 46-47 0.40594691890270695 1106 48-49 0.3698520548947499 1106 50-51 0.28332584984715226 1106 52-53 0.3627172033254169 1106 54-55 0.3828283226096332 1106 56-57 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-0.5009039988829542 2216 116-117 -0.5480423873995584 2216 118-119 -0.6581905908110066 2216 120-121 -0.6191679388180944 2216 122-123 -0.6718876417502422 2216 124-125 -0.8497006733745565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 8.0 4 13.0 5 53.0 6 125.0 7 299.0 8 571.0 9 916.0 10 1890.0 11 2890.0 12 3589.0 13 5665.0 14 10338.0 15 10912.0 16 4536.0 17 3249.0 18 2848.0 19 2517.0 20 2166.0 21 2053.0 22 1933.0 23 2091.0 24 2540.0 25 3092.0 26 3757.0 27 4595.0 28 5693.0 29 6709.0 30 8433.0 31 10396.0 32 13540.0 33 17617.0 34 25828.0 35 48205.0 36 110765.0 37 392285.0 38 310391.0 39 3105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79320967488705 18.615176228401136 13.865108210079583 22.726505886632232 2 15.366637025249808 20.07152941910623 41.55258621362101 23.00924734202295 3 19.089721339287454 25.897603400869716 29.300813166669915 25.71186209317291 4 12.074882903924097 16.774739078600103 38.308700113947154 32.84167790352865 5 12.88530270160598 38.1239952866262 34.06241708676042 14.928284925007413 6 30.515295629694712 37.489821928855406 17.677810293430742 14.317072148019141 7 27.26569827870307 33.657526847123016 21.150579843256093 17.926195030917825 8 25.71619953550138 35.396276600930946 19.83830300052066 19.049220863047008 9 26.873643506087035 16.095891078779513 20.141436123640094 36.88902929149336 10-11 24.58556856229683 27.117885536776708 27.32561314012375 20.970932760802715 12-13 26.01289753924935 24.428688152165222 28.12795552357496 21.43045878501046 14-15 22.726404074795585 25.160244222530327 27.354327422773693 24.759024279900395 16-17 21.176564075263595 28.52283784864209 27.770318889415396 22.530279186678914 18-19 21.040500301280588 26.386280715507272 31.445503314086476 21.127715669125664 20-21 22.1740563287449 25.515102055143124 31.535253728956675 20.775587887155307 22-23 21.553652320250542 25.705425585644615 31.413912728081105 21.327009366023738 24-25 21.183876710912987 25.73813744244956 31.73103736074304 21.346948485894412 26-27 21.25973311804934 25.864256031949395 31.512682060252217 21.36332878974905 28-29 21.07321477409507 25.984149092329073 31.6194748637287 21.32316126984715 30-31 21.29054368958544 25.775090627931146 31.569428112891494 21.364937569591916 32-33 21.197624465933842 25.776504404156697 31.52189598117045 21.50397514873901 34-35 21.512555881105484 25.987529433559374 31.251054245501482 21.24886043983366 36-37 21.66052398085042 25.69597605327561 31.156579986544592 21.486919979329375 38-39 21.439478000066302 25.90269638862942 31.182807231711575 21.475018379592704 40-41 21.686411605060933 25.779052018702806 30.95693684268514 21.57759953355112 42-43 21.65928407554131 25.86492344563387 30.737531091222696 21.738261387602122 44-45 21.79490617079436 26.05854934631808 30.46961647833224 21.67692800455532 46-47 21.91207965121636 26.38273476131302 29.719638287613904 21.98554729985672 48-49 22.08057113717491 26.318955775285367 29.543254066144502 22.05721902139522 50-51 22.162484978341997 26.68304728196353 29.163150440838432 21.99131729885604 52-53 22.216615868224356 26.778579242644952 28.9428268894388 22.061977999691894 54-55 22.08381840483141 26.664648379227433 29.06870172441699 22.18283149152417 56-57 22.082619325619206 26.932281598954766 28.865477290288926 22.119621785137102 58-59 22.105212353330046 26.70451110664761 28.941343718128476 22.248932821893874 60-61 22.15885075279553 26.578608545836847 29.254770695112814 22.007770006254813 62-63 22.191200351397033 26.633730507531784 29.282614768328052 21.892454372743135 64-65 22.05678599460806 26.554862739553727 29.150550163074477 22.237801102763736 66-67 22.099574644419796 26.767809479701153 28.976392155906836 22.15622371997221 68-69 22.25596364311793 26.528979568754412 29.16696933192474 22.048087456202914 70-71 22.0700471280736 26.480595197294875 29.02961604782327 22.419741626808253 72-73 22.160016068298066 26.619651294531515 28.86341166628641 22.35692097088401 74-75 22.06389004130666 26.899445848200337 28.65433300686462 22.382331103628385 76-77 22.17947130352708 26.723977382532212 28.737376642852475 22.359174671088233 78-79 22.102889736620803 26.638862143353364 28.808346232129583 22.449901887896257 80-81 21.943952060310718 26.9758254015612 28.686348381762482 22.3938741563656 82-83 22.133251821821297 26.79686083176843 28.6449101130826 22.424977233327677 84-85 22.2549125235163 26.65888924585506 28.46950401148185 22.616694219146787 86-87 22.052232821236338 26.618984915945926 28.60968419574706 22.719098067070682 88-89 22.21217953593039 26.65723824176938 28.521375321512217 22.60920690078801 90-91 22.13770973878265 26.802054560115284 28.593067029633758 22.46716867146831 92-93 21.947953179311924 26.697396196428485 28.63652540183207 22.718125222427517 94-95 21.934511984360487 26.724746065137005 28.57462528306394 22.766116667438567 96-97 22.036382959534134 26.675246863474932 28.54953235489839 22.738837822092545 98-99 22.06500055576031 26.545988678089977 28.392867937700245 22.996142828449468 100-101 21.96129075033114 26.557727736526328 28.54901008706437 22.931971426078164 102-103 22.10946138050861 26.191033343668522 28.715306418934073 22.984198856888796 104-105 21.96345242204243 26.38242939406526 28.59060160565978 23.063516578232527 106-107 21.881051229600104 26.488078407360245 28.52606916025265 23.104801202787005 108-109 21.95902564017562 26.447543283701417 28.319733389754653 23.2736976863683 110-111 21.840112634089362 26.549986252244977 28.409735750598173 23.200165363067484 112-113 21.875656615343214 26.49554732529949 28.387915791382675 23.240880267974624 114-115 21.785480798810063 26.50695860620193 28.30814727566923 23.39941331931878 116-117 21.640193171525045 26.472406366119127 28.59893117996289 23.28846928239294 118-119 21.511155702068702 26.642642150740187 28.387637124507247 23.45856502268386 120-121 21.49160289639014 26.858592570456057 28.266275619222103 23.3835289139317 122-123 21.615851874963436 26.712768861761667 28.175152590628105 23.496226672646788 124-125 21.483973943337194 26.75780583476581 28.192219077213487 23.56600114468351 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 270.0 2 496.5 3 812.0 4 478.0 5 261.5 6 741.0 7 2031.5 8 3555.5 9 3658.5 10 2272.5 11 1198.5 12 1023.5 13 1125.5 14 1215.5 15 1260.5 16 1390.0 17 1562.0 18 1663.0 19 1779.5 20 1942.5 21 2269.0 22 2751.0 23 3210.5 24 3793.5 25 4586.5 26 5761.5 27 7095.5 28 8200.0 29 9820.5 30 11720.5 31 13653.0 32 15925.5 33 18107.0 34 20332.5 35 22472.5 36 24941.5 37 27589.5 38 29233.5 39 30078.5 40 31001.5 41 32523.0 42 34298.5 43 35611.5 44 36824.5 45 38371.5 46 39421.5 47 39729.5 48 39760.0 49 39144.5 50 38239.5 51 36977.0 52 35778.5 53 35403.5 54 33892.0 55 30648.5 56 27021.5 57 23748.0 58 21440.0 59 19093.5 60 15708.0 61 12395.0 62 9877.0 63 7485.5 64 5397.5 65 3977.0 66 2858.5 67 2151.5 68 1595.5 69 1120.0 70 890.0 71 707.5 72 551.5 73 497.0 74 386.5 75 269.0 76 181.0 77 88.5 78 43.0 79 21.0 80 9.5 81 3.5 82 1.0 83 0.5 84 0.0 85 1.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01589279481134375 2 0.0029250542597565187 3 1.9500361731710123E-4 4 0.14264514606745954 5 0.04426582113098198 6 0.012967740551587233 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.4375452164637654E-4 30-31 0.0 32-33 0.0 34-35 6.825126606098543E-4 36-37 0.0011700217039026075 38-39 0.002730050642439417 40-41 4.875090432927531E-5 42-43 0.0010725198952440567 44-45 8.287653735976802E-4 46-47 4.875090432927531E-5 48-49 0.0018525343645124617 50-51 0.0019012852688417368 52-53 0.0 54-55 0.0 56-57 0.001413776225548984 58-59 0.0019500361731710123 60-61 0.0011212707995733322 62-63 1.4625271298782593E-4 64-65 0.0016575307471953604 66-67 9.262671822562309E-4 68-69 0.0011212707995733322 70-71 0.0012187726082318827 72-73 0.0 74-75 4.875090432927531E-5 76-77 0.004192577772317676 78-79 9.262671822562309E-4 80-81 0.0 82-83 0.0 84-85 3.4125633030492714E-4 86-87 2.4375452164637654E-4 88-89 0.0 90-91 9.750180865855062E-5 92-93 6.825126606098543E-4 94-95 0.001413776225548984 96-97 4.387581389634778E-4 98-99 0.0 100-101 1.4625271298782593E-4 102-103 0.0 104-105 0.0 106-107 3.9000723463420247E-4 108-109 4.875090432927531E-4 110-111 0.0 112-113 2.4375452164637654E-4 114-115 0.002096288886158838 116-117 4.875090432927531E-4 118-119 2.9250542597565187E-4 120-121 4.875090432927531E-5 122-123 1.9500361731710123E-4 124-125 0.0010725198952440567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1025622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.08316317533315 #Duplication Level Percentage of deduplicated Percentage of total 1 68.2900161887392 34.88470040215507 2 15.98851368781089 16.334877072909823 3 6.424377955462635 9.845326421967327 4 3.2608805564621104 6.663043742441005 5 1.848801469308178 4.722131356773266 6 1.0997179476914778 3.3706242821259744 7 0.7287726992358758 2.605961029895596 8 0.4876280462842815 1.9927666445767083 9 0.3584014440458757 1.6477451503623453 >10 1.4438296705560199 12.164487111639003 >50 0.045982250083378924 1.6206501080877795 >100 0.021552260952687346 2.002670987922686 >500 3.8145584184777427E-4 0.13620693738292453 >1k 7.629116836955485E-4 0.7148428466226876 >5k 3.8145584184777427E-4 1.293965905137783 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8030 0.7829395235281614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5251 0.5119819972660493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3874 0.3777220067432251 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.1194397156067245 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1223 0.11924471198940739 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.750180865855062E-5 0.0 20-21 0.0 0.0 0.0 2.4375452164637654E-4 0.0 22-23 0.0 0.0 0.0 5.362599476220285E-4 0.0 24-25 0.0 0.0 0.0 8.775162779269556E-4 0.0 26-27 0.0 0.0 0.0 0.0015112780342075344 0.0 28-29 0.0 0.0 0.0 0.0026325488337808666 0.0 30-31 0.0 0.0 0.0 0.006727624797439992 0.0 32-33 0.0 0.0 0.0 0.01350400049920926 0.0 34-35 0.0 0.0 0.0 0.021937906948173887 0.0 36-37 0.0 0.0 0.0 0.03319936584823649 0.0 38-39 0.0 0.0 0.0 0.058159828864825444 0.0 40-41 0.0 0.0 0.0 0.08721536784507353 0.0 42-43 0.0 0.0 0.0 0.11553964326038248 0.0 44-45 0.0 0.0 0.0 0.1467402220311187 0.0 46-47 0.0 0.0 0.0 0.18471717650362413 0.0 48-49 0.0 0.0 0.0 0.22903174853893538 0.0 50-51 0.0 0.0 0.0 0.28143897069290635 0.0 52-53 0.0 0.0 0.0 0.333261181994926 0.0 54-55 0.0 0.0 0.0 0.38610716228786046 0.0 56-57 0.0 0.0 0.0 0.4450957565262835 0.0 58-59 0.0 0.0 0.0 0.5132982716829397 0.0 60-61 0.0 0.0 0.0 0.5949072855301465 0.0 62-63 0.0 0.0 0.0 0.6768088048033292 0.0 64-65 0.0 0.0 0.0 0.7558340207210843 0.0 66-67 0.0 0.0 0.0 0.8452431792609753 0.0 68-69 0.0 0.0 0.0 0.9497163672386123 0.0 70-71 0.0 0.0 0.0 1.0676448048111293 0.0 72-73 0.0 0.0 0.0 1.2019535462382827 0.0 74-75 0.0 0.0 0.0 1.3270483667472033 0.0 76-77 0.0 0.0 0.0 1.4572620322106975 0.0 78-79 0.0 0.0 0.0 1.6161412294198056 0.0 80-81 0.0 0.0 0.0 1.8009559077320885 0.0 82-83 0.0 0.0 0.0 2.0002008537258367 0.0 84-85 0.0 0.0 0.0 2.1987632870589753 0.0 86-87 0.0 0.0 0.0 2.3968382113488205 0.0 88-89 0.0 0.0 0.0 2.6244561836622067 0.0 90-91 0.0 0.0 0.0 2.8763521063315727 0.0 92-93 0.0 0.0 0.0 3.143409560247343 0.0 94-95 0.0 0.0 0.0 3.4251410363662247 0.0 96-97 0.0 0.0 0.0 3.693076006559922 0.0 98-99 0.0 0.0 0.0 3.9941615916975257 0.0 100-101 0.0 0.0 0.0 4.346484377285199 0.0 102-103 0.0 0.0 0.0 4.720745069821045 0.0 104-105 0.0 0.0 0.0 5.101050874493722 0.0 106-107 0.0 0.0 0.0 5.481356679166399 0.0 108-109 0.0 0.0 0.0 5.8821866145617 0.0 110-111 0.0 0.0 0.0 6.336740046527863 0.0 112-113 0.0 0.0 0.0 6.838240599363118 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 65.01687 1 GTATCAA 5425 0.0 56.138226 1 ATCAACG 6690 0.0 44.892967 3 TCAACGC 6785 0.0 44.088745 4 CAACGCA 6860 0.0 43.640316 5 TATCAAC 6920 0.0 43.486973 2 AACGCAG 7075 0.0 42.21767 6 TAACGCA 145 5.820766E-11 41.096596 4 TATAACG 160 3.6379788E-12 40.96817 2 ATAACGC 170 7.2759576E-12 38.55828 3 ACGCAGA 8020 0.0 37.16533 7 CGCAGAG 8155 0.0 36.47713 8 TATCACG 100 1.0511221E-5 35.75404 2 ATCACGC 100 1.0511221E-5 35.75404 3 GTGGTAT 1265 0.0 34.392982 1 TGGTATC 1305 0.0 33.333904 2 GTACATG 5180 0.0 33.020954 1 TACATGG 5175 0.0 32.93287 2 GCAGAGT 9075 0.0 32.779175 9 ACATGGG 5170 0.0 31.120539 3 >>END_MODULE