FastQCFastQC Report
Fri 27 May 2016
SRR940027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729334
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26150.048.3640941
GGTATCA14750.045.298611
ATCAACG27800.043.741293
CAACGCA27900.043.5693555
TCAACGC27950.043.510334
AACGCAG30450.040.1163836
TATCAAC32550.037.938332
ACGCAGA35900.033.8603067
GTACATG67300.033.5070081
CGCGTAC452.3977466E-433.04670786-87
TACATGG67900.031.8051662
CGCAGAG38500.031.5736358
ACATGGG71150.029.9926173
TCTAACG1159.897153E-425.9376742
CATGGGG47600.025.924324
AGGACCG5300.025.8587275
GCAGAGT48250.024.3198269
GGACCGA4200.022.76
AGAGTAC43650.020.85672610-11
GAGTACA50200.020.7966421
Produced by FastQC (version 0.11.5)