FastQCFastQC Report
Fri 27 May 2016
SRR940027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729334
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.23037770609957356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.1705280761264163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.10287197267849935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.064.3835141
GTATCAA48300.058.7463381
ATCAACG59650.046.560863
TCAACGC59950.046.2284434
TATCAAC61350.045.3691252
CAACGCA61550.045.0648275
AACGCAG65600.042.267956
ACGCAGA74650.037.059717
CGCAGAG77600.035.497538
GTACATG67300.034.632411
TACATGG71550.032.0707552
ACATGGG68700.032.0121733
GCAGAGT89000.031.0175049
CATGGGG43750.028.7441794
ATTCCGT2552.910383E-1128.0007826
TAACGCA2153.965397E-927.7208824
GAGTACT56000.027.14449512-13
GCGTATC900.00945131326.489612
TATAACG2057.646668E-826.1665672
GTGTACG1859.115083E-725.7774071
Produced by FastQC (version 0.11.5)