FastQCFastQC Report
Fri 27 May 2016
SRR940026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418645
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31540.7533829378112721No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.14929116554598765No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.1488134338162405No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA350.00770815951.1410752
GTATCAA13800.047.9968381
GGTATCA8250.044.844221
ATCAACG15750.039.7430573
TCAACGC15900.039.3728374
CAACGCA16350.038.6399575
TATCAAC17400.037.3760952
AACGCAG16550.037.092646
GTACATG33050.036.6516271
TACATGG32750.035.8898432
ACATGGG34400.032.2334673
GGCCCGC1301.7147449E-632.0809569
GTGTAGG2450.031.6625561
ACGCAGA19550.031.0958147
CGCAGAG19750.030.780928
CATGGGG11300.030.0746754
AGGCCCG1403.0479277E-629.8001568
ACGAATC703.1014897E-629.73963572-73
AAGCGTT850.007146192728.0472057
ATGGGAT7350.027.5702135
Produced by FastQC (version 0.11.5)