##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418645 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.614912395944057 33.0 31.0 34.0 26.0 34.0 2 30.57734596137539 34.0 31.0 34.0 26.0 34.0 3 30.81139390175447 34.0 31.0 34.0 26.0 34.0 4 33.740617945992426 37.0 35.0 37.0 26.0 37.0 5 34.10452292515138 37.0 35.0 37.0 30.0 37.0 6 33.966969628205284 37.0 36.0 37.0 29.0 37.0 7 33.981631214991225 37.0 35.0 37.0 30.0 37.0 8 33.82134744234375 37.0 35.0 37.0 28.0 37.0 9 35.24945001134613 39.0 38.0 39.0 25.0 39.0 10-11 35.37230828028521 39.0 38.0 39.0 27.0 39.0 12-13 35.30837583155179 39.0 38.0 39.0 27.0 39.0 14-15 36.726125953970545 41.0 39.0 41.0 27.0 41.0 16-17 36.8210751352578 41.0 39.0 41.0 27.0 41.0 18-19 36.83392014714137 41.0 39.0 41.0 27.0 41.0 20-21 36.89508294617158 41.0 39.0 41.0 27.5 41.0 22-23 36.96762173201638 41.0 39.0 41.0 28.0 41.0 24-25 37.087901443944155 41.0 39.0 41.0 29.0 41.0 26-27 37.19300003582988 41.0 39.0 41.0 30.0 41.0 28-29 37.168173512164245 41.0 38.0 41.0 30.0 41.0 30-31 37.119850947700314 41.0 38.0 41.0 30.0 41.0 32-33 37.08690776194628 41.0 38.0 41.0 30.0 41.0 34-35 37.003985476955414 41.0 38.0 41.0 30.0 41.0 36-37 36.84901169248408 41.0 38.0 41.0 29.0 41.0 38-39 36.74096191283785 41.0 38.0 41.0 29.0 41.0 40-41 36.653384132140594 41.0 38.0 41.0 28.5 41.0 42-43 36.471026764920154 40.0 37.0 41.0 28.0 41.0 44-45 36.313021772623586 40.0 37.0 41.0 28.0 41.0 46-47 36.161445855080075 40.0 36.5 41.0 27.5 41.0 48-49 36.02218586152946 40.0 36.0 41.0 27.0 41.0 50-51 35.86194866772564 40.0 35.5 41.0 27.0 41.0 52-53 35.98992463781963 40.0 35.5 41.0 28.0 41.0 54-55 35.79550335009375 40.0 35.0 41.0 28.0 41.0 56-57 35.67780816682392 40.0 35.0 41.0 28.0 41.0 58-59 35.47608952692616 39.0 35.0 41.0 28.0 41.0 60-61 35.236278947557 39.0 35.0 41.0 28.0 41.0 62-63 34.937203358454056 39.0 35.0 41.0 27.5 41.0 64-65 34.61330721733211 37.5 35.0 41.0 27.0 41.0 66-67 34.276867035316315 37.0 35.0 40.0 26.0 41.0 68-69 33.91812275316796 37.0 34.0 40.0 26.0 41.0 70-71 33.544584313678655 36.0 34.0 39.0 25.0 41.0 72-73 33.18122156003297 36.0 34.0 39.0 25.0 41.0 74-75 32.818463137025404 35.0 34.0 38.0 24.5 40.0 76-77 32.46986587681687 35.0 34.0 37.0 24.0 39.0 78-79 32.13577613491144 35.0 33.0 37.0 23.0 39.0 80-81 31.83794384263517 35.0 33.0 36.0 21.5 39.0 82-83 31.556107203000153 35.0 33.0 36.0 20.0 37.0 84-85 31.326087735432168 35.0 33.0 35.5 20.0 37.0 86-87 31.13133800714209 35.0 33.0 35.0 18.5 36.5 88-89 30.95883505117701 35.0 33.0 35.0 18.0 36.0 90-91 30.81661073224331 35.0 33.0 35.0 16.5 36.0 92-93 30.6684410419329 35.0 32.5 35.0 13.5 36.0 94-95 30.55896642740269 35.0 32.0 35.0 10.0 35.5 96-97 30.45581578664501 35.0 32.0 35.0 7.0 35.0 98-99 30.37776158798027 35.0 32.0 35.0 7.0 35.0 100-101 30.285939160864217 35.0 32.0 35.0 6.0 35.0 102-103 29.601108337613013 34.0 30.0 34.5 5.0 35.0 104-105 30.469784662422818 35.0 32.5 35.0 2.0 35.0 106-107 30.564791171517633 35.0 33.0 35.0 2.0 35.0 108-109 30.540376691468907 35.0 33.0 35.0 2.0 35.0 110-111 30.494067766245863 35.0 33.0 35.0 2.0 35.0 112-113 30.445673542022476 35.0 33.0 35.0 2.0 35.0 114-115 30.379673709228584 35.0 33.0 35.0 2.0 35.0 116-117 30.29928340240538 35.0 33.0 35.0 2.0 35.0 118-119 30.209817387046307 35.0 33.0 35.0 2.0 35.0 120-121 30.124321322361425 35.0 33.0 35.0 2.0 35.0 122-123 29.997770187151406 35.0 33.0 35.0 2.0 35.0 124-125 28.806982049230253 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07716639357191113 1101 2 -0.11975866422983117 1101 3 0.20178463797768842 1101 4 -0.134150547050929 1101 5 -0.05164058763454449 1101 6 0.37404221157107287 1101 7 -0.066549825182868 1101 8 0.07311767836645089 1101 9 -0.10384660047775185 1101 10-11 -0.10383922616312447 1101 12-13 -0.07496700144996993 1101 14-15 -0.13897542342203195 1101 16-17 -0.05095108230707268 1101 18-19 -0.21513346882715467 1101 20-21 -0.09342434309518666 1101 22-23 -0.15836751731284693 1101 24-25 -0.22092297771966685 1101 26-27 -0.2932790086440491 1101 28-29 -0.1810767239037716 1101 30-31 -0.2028628928405638 1101 32-33 -0.06477671948894681 1101 34-35 -0.04687354508422459 1101 36-37 -0.05217385005568076 1101 38-39 -0.027441372583119517 1101 40-41 0.0015442978364532678 1101 42-43 0.019434438529472686 1101 44-45 0.09365302371810458 1101 46-47 0.01082510843258433 1101 48-49 0.1048580225356659 1101 50-51 0.12841545335009386 1101 52-53 0.17970312695643287 1101 54-55 0.23134468059542002 1101 56-57 0.12310372613488596 1101 58-59 0.18203614713184635 1101 60-61 0.24904531492387605 1101 62-63 0.048381201494855475 1101 64-65 0.19552821031363266 1101 66-67 0.23114976396159648 1101 68-69 0.042050125102989 1101 70-71 0.1511090074971122 1101 72-73 0.05361862380739524 1101 74-75 0.05866013309794482 1101 76-77 -0.15934654835588447 1101 78-79 -0.05165717396027247 1101 80-81 -0.11981062589238434 1101 82-83 0.007255598934804652 1101 84-85 -0.1252123245839485 1101 86-87 -0.18680027276379363 1101 88-89 -0.17903864533344205 1101 90-91 -0.42270673501052514 1101 92-93 -0.3935109948640765 1101 94-95 -0.31234030214749353 1101 96-97 -0.218932447744713 1101 98-99 -0.15852083630318248 1101 100-101 -0.1522888351029117 1101 102-103 -0.22432850274578442 1101 104-105 -0.09733494693818301 1101 106-107 -0.11352301417539934 1101 108-109 -0.2137594691832092 1101 110-111 -0.2961213842149455 1101 112-113 -0.20834896202188702 1101 114-115 -0.18913584972621322 1101 116-117 -0.1648842843296272 1101 118-119 -0.017310349283103932 1101 120-121 -0.0783797661313379 1101 122-123 -0.05518275699776254 1101 124-125 -0.023206445532586883 1102 1 0.6378590424746022 1102 2 0.7392983851887749 1102 3 0.7779867939854412 1102 4 0.8081035711661286 1102 5 0.7319107689546058 1102 6 0.9587381902532428 1102 7 0.6752288812124831 1102 8 0.7969397471648989 1102 9 1.2978612483594603 1102 10-11 1.0144383901159486 1102 12-13 0.9270164045577829 1102 14-15 1.0640133120430804 1102 16-17 1.0762442907549428 1102 18-19 0.9126826648937723 1102 20-21 0.9880391380094622 1102 22-23 0.8958354865631222 1102 24-25 0.9265606705361478 1102 26-27 1.1074068561815338 1102 28-29 1.0826405726078576 1102 30-31 1.2614282287679615 1102 32-33 1.2127941380691922 1102 34-35 1.1647831288498836 1102 36-37 1.2295682163202883 1102 38-39 1.2946274258664943 1102 40-41 1.1932987794255965 1102 42-43 1.2929400707969165 1102 44-45 1.0910564273033714 1102 46-47 1.11200152558375 1102 48-49 1.0133412468573724 1102 50-51 1.113850288621414 1102 52-53 1.125507693332402 1102 54-55 1.0627854509442543 1102 56-57 0.997763064797681 1102 58-59 0.868022096744248 1102 60-61 1.065527267443258 1102 62-63 1.0904718022700521 1102 64-65 1.094692454499679 1102 66-67 1.2411424965197355 1102 68-69 1.0889326953549272 1102 70-71 1.0270250079816066 1102 72-73 0.9122138878577886 1102 74-75 0.9359236384274041 1102 76-77 0.9487945005782237 1102 78-79 0.9034133807877893 1102 80-81 0.9418415058905616 1102 82-83 1.0178539516479823 1102 84-85 1.0104087388850438 1102 86-87 0.9194482131858202 1102 88-89 0.8741120935231486 1102 90-91 1.0796778943499383 1102 92-93 0.8795025710273983 1102 94-95 0.8646976096742129 1102 96-97 0.8437492383017968 1102 98-99 0.6335016322045703 1102 100-101 0.6538370127653046 1102 102-103 0.7642934085914277 1102 104-105 0.9167230109106548 1102 106-107 0.8259982430920445 1102 108-109 0.7647485855648526 1102 110-111 0.8513092722773017 1102 112-113 0.9198305437920666 1102 114-115 0.8362169240140958 1102 116-117 0.7409569220657239 1102 118-119 0.9602287132168961 1102 120-121 1.0084131723376544 1102 122-123 0.8812574077309172 1102 124-125 0.6482294798550114 1103 1 0.047866765858632476 1103 2 0.16618615847774976 1103 3 0.16368813666604964 1103 4 0.16416642763000766 1103 5 0.3296412822625925 1103 6 0.48352728245856724 1103 7 0.15307613644061746 1103 8 -0.010517774222932985 1103 9 0.33971604873968886 1103 10-11 0.19151182167488656 1103 12-13 0.20252585085055586 1103 14-15 0.30004438381114085 1103 16-17 0.35530352316939684 1103 18-19 0.1439831639431972 1103 20-21 0.2021588207566154 1103 22-23 0.21686091431977417 1103 24-25 0.16637917101142818 1103 26-27 0.06484284121005146 1103 28-29 0.17622719333029124 1103 30-31 0.134033237085454 1103 32-33 0.1590052240958073 1103 34-35 0.1505208605514028 1103 36-37 0.13484165382028834 1103 38-39 0.333303756664975 1103 40-41 0.346229209558679 1103 42-43 0.39189221552125275 1103 44-45 0.3765877070086958 1103 46-47 0.2237418119430714 1103 48-49 0.2557923816007204 1103 50-51 0.113954257252594 1103 52-53 0.031642585205027274 1103 54-55 0.24466371852980018 1103 56-57 0.04075780695547593 1103 58-59 0.11110075644006656 1103 60-61 0.1528534913025723 1103 62-63 0.062400271851807076 1103 64-65 0.12626921305481176 1103 66-67 0.29048303834483136 1103 68-69 0.39754501118192564 1103 70-71 0.24060999491126722 1103 72-73 0.4000978291600745 1103 74-75 0.33404551936846616 1103 76-77 0.33795280024552454 1103 78-79 0.29294299699501636 1103 80-81 0.20972411045330475 1103 82-83 0.23621778244646308 1103 84-85 0.3009379392177429 1103 86-87 0.25474333557174944 1103 88-89 0.36842723729690974 1103 90-91 0.3495321897396728 1103 92-93 0.3551442060083865 1103 94-95 0.1575104067274431 1103 96-97 0.2777737154881095 1103 98-99 0.3138389361494376 1103 100-101 0.37272811305047426 1103 102-103 0.31904403240321244 1103 104-105 0.4221627982205405 1103 106-107 0.3093149909532613 1103 108-109 0.24205743181009964 1103 110-111 0.3712526836366159 1103 112-113 0.3613853204080364 1103 114-115 0.2415646248220824 1103 116-117 0.5423842628451929 1103 118-119 0.16995200813134304 1103 120-121 0.1676374178224478 1103 122-123 0.10851798282597613 1103 124-125 -0.027366111875025467 1104 1 0.015256568765234846 1104 2 0.09971530081974933 1104 3 0.060241364970146094 1104 4 0.09671046648161763 1104 5 0.1580878722050869 1104 6 0.19548084082685335 1104 7 0.22095740057194746 1104 8 0.3211204056256989 1104 9 0.1902638535219836 1104 10-11 0.14023009661691077 1104 12-13 0.2390346586208807 1104 14-15 0.045053435131030994 1104 16-17 0.11641592674921242 1104 18-19 0.26809166345973523 1104 20-21 0.23809328774655825 1104 22-23 0.20360018541589398 1104 24-25 0.33807253119580594 1104 26-27 0.17060333383449944 1104 28-29 0.31502136849695717 1104 30-31 0.4347951473865095 1104 32-33 0.3899011230118319 1104 34-35 0.37899762156020955 1104 36-37 0.35185886223328566 1104 38-39 0.3357065367651586 1104 40-41 0.35013509037205637 1104 42-43 0.35583779127971127 1104 44-45 0.418847192719241 1104 46-47 0.400109869871514 1104 48-49 0.39046802446253537 1104 50-51 0.2544678196921595 1104 52-53 0.3043381337004689 1104 54-55 0.4161516973591688 1104 56-57 0.25251143657110475 1104 58-59 0.1585783825951026 1104 60-61 0.2250150482749973 1104 62-63 0.23081298295839048 1104 64-65 0.2530785682664103 1104 66-67 0.3779882183170571 1104 68-69 0.3154597179409677 1104 70-71 0.335247178751203 1104 72-73 0.2162833339619965 1104 74-75 0.340872177504842 1104 76-77 0.2376049242158942 1104 78-79 0.1828204224942951 1104 80-81 0.299184957133388 1104 82-83 0.1578366237321127 1104 84-85 0.21008122633724113 1104 86-87 0.2829441949018836 1104 88-89 0.28917729727553976 1104 90-91 0.32105688275816036 1104 92-93 0.25802790563542644 1104 94-95 0.2956884378290887 1104 96-97 0.1177543769251237 1104 98-99 0.20423799393974917 1104 100-101 0.17316286721080942 1104 102-103 0.1744664636822506 1104 104-105 0.15128660137432703 1104 106-107 -0.0036970992836700134 1104 108-109 0.07186535480481382 1104 110-111 0.07534540695703384 1104 112-113 0.04695918862954329 1104 114-115 0.13645480941084287 1104 116-117 0.08390401217088694 1104 118-119 0.14760360865188815 1104 120-121 0.07598644253364029 1104 122-123 0.1256482418609366 1104 124-125 0.09298554462556297 1105 1 -0.13646656981745053 1105 2 -0.06150091587407047 1105 3 0.08145765234958091 1105 4 0.013329714881471943 1105 5 -0.4067502754076884 1105 6 -0.45687670115156465 1105 7 -0.14361426227180374 1105 8 -0.2937596232140294 1105 9 -0.14860651966272087 1105 10-11 -0.08926135572509253 1105 12-13 -0.02200378375090395 1105 14-15 -0.044130605470783735 1105 16-17 0.0441964337771239 1105 18-19 0.0764346866128065 1105 20-21 -0.27190519251271894 1105 22-23 -0.37615792840914963 1105 24-25 -0.48884843875220696 1105 26-27 -0.25228042720571153 1105 28-29 -0.17302746112596878 1105 30-31 -0.1487623182514497 1105 32-33 -0.08922541484208324 1105 34-35 -0.15810525781832752 1105 36-37 -0.02227479719634573 1105 38-39 -0.03320875712796578 1105 40-41 -0.08762269123059951 1105 42-43 0.029197796651814656 1105 44-45 -0.02453716384727045 1105 46-47 0.08103853748301049 1105 48-49 0.009624533017266401 1105 50-51 -0.08094956119374075 1105 52-53 -0.07944587933395297 1105 54-55 -0.05559702941618383 1105 56-57 -0.13897554137145107 1105 58-59 -0.07587519992858205 1105 60-61 -0.11092187188206992 1105 62-63 -0.03538020789630281 1105 64-65 -0.05507360372583037 1105 66-67 0.020933970641728195 1105 68-69 0.05309592028561383 1105 70-71 -5.660415793897755E-4 1105 72-73 -0.14209273471615091 1105 74-75 -0.01410921450698055 1105 76-77 -0.07903482301234988 1105 78-79 -0.14241646267338837 1105 80-81 -0.15522988967320828 1105 82-83 -0.11412097903593477 1105 84-85 -0.16192656321754484 1105 86-87 -0.05070074418479109 1105 88-89 0.025984710782854137 1105 90-91 0.1627723519515989 1105 92-93 0.19425534367065111 1105 94-95 0.18276816766866588 1105 96-97 0.1445954721280458 1105 98-99 0.13104127233858165 1105 100-101 -0.0360276764675973 1105 102-103 -0.09302557535034595 1105 104-105 0.1261207552498398 1105 106-107 0.12636973396542572 1105 108-109 0.10721876520902995 1105 110-111 0.040933882374343256 1105 112-113 0.13536745460537247 1105 114-115 0.06663332593183924 1105 116-117 0.07551893107681451 1105 118-119 -0.08801275224059069 1105 120-121 0.05981357379329211 1105 122-123 0.019246952555317165 1105 124-125 0.0396956593836606 1106 1 0.03661482951163819 1106 2 0.35056863981840536 1106 3 0.3479086689854398 1106 4 0.23510009894390294 1106 5 0.2056376208064563 1106 6 0.479253236549539 1106 7 0.42651649695322646 1106 8 0.47389287216489606 1106 9 0.616437637248346 1106 10-11 0.5112555197455819 1106 12-13 0.4868977702985191 1106 14-15 0.589577548154189 1106 16-17 0.36433746205123896 1106 18-19 0.5744014148937708 1106 20-21 0.6005463718057555 1106 22-23 0.4712405791557117 1106 24-25 0.37894782331392207 1106 26-27 0.3393006640518976 1106 28-29 0.3769982114967476 1106 30-31 0.36602168941610813 1106 32-33 0.3089312908469708 1106 34-35 0.4286647839424731 1106 36-37 0.3292860982647312 1106 38-39 0.4403450184590838 1106 40-41 0.39585954331447937 1106 42-43 0.29219498977839464 1106 44-45 0.277869216655219 1106 46-47 0.29635482419486436 1106 48-49 0.40048679083885474 1106 50-51 0.35664542751030837 1106 52-53 0.3565334456472158 1106 54-55 0.3791482981664771 1106 56-57 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-0.360206353681356 2216 10-11 -0.12551696702691117 2216 12-13 -0.10690419493200665 2216 14-15 -0.20163560377324785 2216 16-17 -0.1901220995511821 2216 18-19 -0.28988429939193594 2216 20-21 -0.22738257755175795 2216 22-23 -0.4070662991511611 2216 24-25 -0.485564648341402 2216 26-27 -0.5137227675429514 2216 28-29 -0.4654621059635673 2216 30-31 -0.6780448260789811 2216 32-33 -0.5361934257063226 2216 34-35 -0.7118869923235849 2216 36-37 -0.8488318155674648 2216 38-39 -0.6838802271947344 2216 40-41 -0.6328252639417471 2216 42-43 -0.6397966957847174 2216 44-45 -0.541329573972142 2216 46-47 -0.6300624093652232 2216 48-49 -0.6748722416630386 2216 50-51 -0.5638761144398146 2216 52-53 -0.7679752467186205 2216 54-55 -0.6053188220149295 2216 56-57 -0.6338018267839516 2216 58-59 -0.6547776481537113 2216 60-61 -0.6575833830669495 2216 62-63 -0.4606442691585144 2216 64-65 -0.4775970863166492 2216 66-67 -0.4938383708272056 2216 68-69 -0.6783066222471135 2216 70-71 -0.7829757892122728 2216 72-73 -0.6532078277034401 2216 74-75 -0.5984154878481078 2216 76-77 -0.5197681588605505 2216 78-79 -0.6073208347734322 2216 80-81 -0.7699569634971937 2216 82-83 -0.8641294667193655 2216 84-85 -0.8020553873904674 2216 86-87 -0.7360962702325473 2216 88-89 -0.6920270965278696 2216 90-91 -0.6304903135582229 2216 92-93 -0.6341453881562735 2216 94-95 -0.6839073010400725 2216 96-97 -0.8008617310859591 2216 98-99 -0.7103559889688995 2216 100-101 -0.8440504234591835 2216 102-103 -0.8148719294187785 2216 104-105 -0.7635902862832218 2216 106-107 -0.8349990464487718 2216 108-109 -0.7969741504045373 2216 110-111 -0.7366331703247404 2216 112-113 -0.6285910123303822 2216 114-115 -0.7082704803226392 2216 116-117 -0.7268444172199899 2216 118-119 -0.6775144309157568 2216 120-121 -0.6434881350603021 2216 122-123 -0.7562585361466354 2216 124-125 -0.7149640788184577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12272.0 3 2487.0 4 2123.0 5 1213.0 6 1014.0 7 1071.0 8 1029.0 9 1107.0 10 1330.0 11 1813.0 12 1871.0 13 1900.0 14 1757.0 15 1672.0 16 1479.0 17 1332.0 18 1116.0 19 986.0 20 871.0 21 812.0 22 930.0 23 1498.0 24 3592.0 25 4968.0 26 5874.0 27 3759.0 28 3459.0 29 4103.0 30 4163.0 31 4893.0 32 6368.0 33 8767.0 34 13508.0 35 25290.0 36 62151.0 37 147774.0 38 77943.0 39 350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71123857322101 19.763398458505108 12.770030471410648 22.755332496863236 2 14.583986727030268 22.731157721856587 39.47702967025863 23.207825880854507 3 18.894838325842777 28.611296284349635 26.998019243680815 25.495846146126773 4 11.898748708529446 18.701211112386638 35.303543412543526 34.09649676654039 5 13.0895293800011 41.67867288839829 30.97222919100764 14.259568540592976 6 30.12527004033877 38.24081862848185 17.353556885311715 14.280354445867665 7 28.128816102968763 35.8380908910593 18.842318326614205 17.190774679357737 8 26.190231362467863 37.91642255036767 18.35045136605488 17.542894721109583 9 26.544832617717763 20.460704931169342 18.121060625994186 34.873401825118705 10-11 24.220917810085346 30.88043039227168 24.747436121115943 20.151215676527034 12-13 24.610916094560594 28.301508066038522 25.46089088445648 21.626684954944405 14-15 22.073598570409825 28.73144178612767 23.602930373177028 25.592029270285476 16-17 21.9302993265064 31.982522858996465 24.257717338188893 21.829460476308245 18-19 22.14436809082761 30.324589184344187 26.050313713773527 21.480729011054674 20-21 23.490377043726347 29.302805389142726 25.610754350734677 21.596063216396246 22-23 22.984591322066 29.46589786698745 25.49295572546103 22.05655508548552 24-25 22.430739917737778 29.542441784714907 25.791155316862564 22.235662980684754 26-27 22.06166232649258 29.717490503380557 25.701913658408394 22.51893351171847 28-29 22.09786902144117 29.65608841632018 25.886952190640226 22.35909037159842 30-31 22.36925873768375 29.38221968999304 25.864929461889353 22.38359211043386 32-33 22.03182930092115 29.384173259947254 25.82762393456408 22.75637350456752 34-35 22.464470940107645 29.551649422719965 25.73835227340594 22.245527363766453 36-37 22.622317986154574 29.165213486152187 25.527922564987026 22.684545962706213 38-39 22.255229031021223 29.27182361225082 25.698965959190208 22.77398139753775 40-41 22.5629216274581 29.009760735375618 25.976073537561874 22.451244099604413 42-43 22.30887483919363 29.13411221148919 25.674072817662 22.882940131655175 44-45 22.171801931472736 29.317534356205616 25.996643513679775 22.514020198641877 46-47 22.368531079250474 29.50599557051694 25.69757190742524 22.427901442807347 48-49 22.351077631003662 29.33304185570902 25.635457058417373 22.680423454869945 50-51 22.426598819847584 29.364533314221553 25.739864784156335 22.46900308177453 52-53 22.630348595667012 29.30805259215991 25.68842955758703 22.373169254586045 54-55 22.328322694086616 29.16794082795 26.02167826153434 22.482058216429035 56-57 22.24603350940944 29.42322532246774 25.78965475226307 22.541086415859752 58-59 22.274279133885546 28.985519365250777 26.04328183123526 22.696919669628414 60-61 22.13692497518523 29.20880598851175 26.16724975901902 22.487019277284006 62-63 22.191520955108057 29.390669442921325 26.038258318311215 22.3795512836594 64-65 21.896199476696257 29.454593244841636 25.927072008028766 22.722135270433338 66-67 21.972191424693673 29.88776690306425 25.508955708906004 22.631085963336073 68-69 22.239706362036397 29.372876961439705 25.92702781173046 22.460388864793433 70-71 22.094219321017817 29.254390733136848 26.01586290853441 22.635527037310926 72-73 22.112880644868433 29.44828294701433 25.828184802396752 22.610651605720484 74-75 21.994675037536325 29.76102170477634 25.760730253264196 22.483573004423135 76-77 22.330202618524613 29.491070714020374 25.768374883090917 22.410351784364106 78-79 22.317662195854773 29.749635330365003 25.714913763545937 22.217788710234284 80-81 22.15001535059688 29.919687110635394 25.671095841481495 22.25920169728623 82-83 22.288562919532236 29.615227937636558 25.84611267804175 22.250096464789458 84-85 22.106389815667598 29.81141496710782 25.688394500728084 22.3938007164965 86-87 22.254186787218742 29.68933310076723 25.804324295910103 22.252155816103926 88-89 22.13576481354709 29.860660604692118 25.60198609114945 22.401588490611346 90-91 22.1314256495539 29.96083800457806 25.78073105577299 22.12700529009505 92-93 22.132057324802716 29.553934658327208 25.933678620285903 22.380329396584166 94-95 21.87635896186066 29.556793256204035 26.03639508933905 22.530452692596256 96-97 22.340378506308635 29.486145608206186 25.817685689947872 22.3557901955373 98-99 21.912389154262858 29.539388320306248 25.757669891776807 22.790552633654094 100-101 21.703416820572123 29.847305081627628 26.004964794873693 22.444313302926552 102-103 21.885528321633206 29.65193518018513 26.00713476729572 22.455401730885942 104-105 21.720015960585954 29.81499578286913 25.83524278966194 22.629745466882977 106-107 21.737920092139802 29.991433579174874 25.733910481499755 22.536735847185565 108-109 21.997859240269218 29.87255789727698 25.521869915396984 22.60771294705681 110-111 21.874458693457733 30.262037164240617 25.645715241400318 22.21778890090133 112-113 21.708255629055206 30.49707242536507 25.69073677394536 22.103935171634365 114-115 21.66342953932453 30.606143135976073 25.4775061457639 22.252921178935498 116-117 21.573335148958943 30.50561589723559 25.66697365073336 22.25407530307211 118-119 21.62653610794511 30.82239569519413 25.320721896064363 22.2303463007964 120-121 21.353975027357134 30.836148689007924 25.538899693695672 22.270976589939266 122-123 21.522439515623265 30.74647868816426 25.52546418242611 22.205617613786362 124-125 21.710517670809864 30.822646828691397 25.43736523655651 22.02947026394223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2755.0 1 2513.5 2 2140.0 3 1631.0 4 1037.0 5 681.0 6 491.5 7 460.5 8 529.0 9 498.0 10 376.5 11 315.5 12 279.0 13 271.5 14 306.0 15 331.0 16 327.5 17 339.0 18 389.5 19 441.0 20 532.0 21 664.5 22 831.0 23 1102.0 24 1425.5 25 1763.0 26 2160.0 27 2605.0 28 3096.0 29 3741.0 30 4563.5 31 5447.5 32 6588.5 33 7350.0 34 7902.5 35 8415.0 36 9248.0 37 10414.0 38 10915.0 39 11015.5 40 10989.5 41 11503.5 42 12022.0 43 12557.5 44 13370.5 45 14101.5 46 14329.0 47 14264.0 48 14574.5 49 14880.0 50 14957.5 51 14842.0 52 14551.5 53 14842.0 54 15067.0 55 14189.5 56 12886.0 57 11469.0 58 10623.0 59 9783.0 60 8559.0 61 7194.0 62 5759.5 63 4297.0 64 3069.0 65 2337.0 66 1741.5 67 1402.0 68 1065.0 69 692.5 70 499.0 71 388.5 72 299.0 73 260.0 74 212.0 75 154.5 76 101.0 77 66.5 78 55.5 79 37.0 80 22.5 81 13.5 82 11.0 83 11.5 84 9.5 85 8.0 86 5.5 87 5.5 88 5.0 89 4.5 90 5.0 91 3.0 92 3.5 93 4.0 94 3.5 95 4.5 96 5.0 97 3.0 98 3.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05255049027218765 2 0.22787803508939555 3 0.02818617205508247 4 0.12349365213964099 5 0.02675297686584099 6 0.04514564846110666 7 0.04490678259623308 8 0.11226695649058271 9 0.14093086027541235 10-11 0.05637234411016494 12-13 0.03869627010952 14-15 0.015167982419472346 16-17 0.03714364198784173 18-19 0.06449378351586667 20-21 0.2011250582235546 22-23 0.12038839589628444 24-25 0.08324475390844271 26-27 0.017914939865518516 28-29 0.008240872338138519 30-31 0.00991293339225358 32-33 0.00883803700032247 34-35 0.01051009805443753 36-37 0.005493914892092345 38-39 0.010629530986874322 40-41 0.006927110081333828 42-43 0.012301592040989384 44-45 0.012540457905862962 46-47 0.0205424643791279 48-49 0.02102019610887506 50-51 0.013137622568046913 52-53 0.015645714149219506 54-55 0.016481744676277037 56-57 0.0181538057303921 58-59 0.0205424643791279 60-61 0.010390665122000741 62-63 0.022811690095426915 64-65 0.03582987973103703 66-67 0.033680086947174816 68-69 0.040726629960945435 70-71 0.02902220258214 72-73 0.018870403325012837 74-75 0.012062726176115802 76-77 0.012062726176115802 78-79 0.027589007392898515 80-81 0.02257282423055333 82-83 0.023528287690047653 84-85 0.01922870212232321 86-87 0.03009709897407111 88-89 0.032127458825496544 90-91 0.03033596483894469 92-93 0.03642704439322099 94-95 0.03033596483894469 96-97 0.0314108612308758 98-99 0.037740806650025674 100-101 0.023408854757610863 102-103 0.03129142829843901 104-105 0.027230708595588148 106-107 0.03594931266347382 108-109 0.024364318217105184 110-111 0.023289421825174076 112-113 0.029738800176760737 114-115 0.015287415351909134 116-117 0.013495921365357284 118-119 0.032485757622806914 120-121 0.025558647541473082 122-123 0.020661897311564693 124-125 0.02090076317643827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 418645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.26121748003351 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27099158394006 54.513760656414576 2 10.607215574503734 14.056940360795808 3 3.123526259607646 6.209059583773734 4 1.3658679097733553 3.620162824739643 5 0.7035046537775506 2.3307537431084975 6 0.4370651129977325 1.73762799031669 7 0.2768716130593236 1.2842095116880068 8 0.2014643125509542 1.0679416502723376 9 0.1484043109624776 0.8850105291293323 >10 0.8076018593848501 9.311738983872166 >50 0.03723988287024635 1.6300269983134017 >100 0.019162269791352094 2.2981847401611013 >500 7.231045204283809E-4 0.29898202394673773 >1k 3.6155226021419043E-4 0.7556004034679573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3154 0.7533829378112721 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 625 0.14929116554598765 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 623 0.1488134338162405 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1943293243679012E-4 0.0 16-17 0.0 0.0 0.0 3.5829879731037035E-4 0.0 18-19 0.0 0.0 0.0 4.777317297471605E-4 0.0 20-21 0.0 0.0 0.0 4.777317297471605E-4 0.0 22-23 0.0 0.0 0.0 5.971646621839505E-4 0.0 24-25 0.0 0.0 0.0 0.0011943293243679013 0.0 26-27 0.0 0.0 0.0 0.0015526281216782715 0.0 28-29 0.0 0.0 0.0 0.002269225716299012 0.0 30-31 0.0 0.0 0.0 0.005255049027218765 0.0 32-33 0.0 0.0 0.0 0.011346128581495061 0.0 34-35 0.0 0.0 0.0 0.018512104527702467 0.0 36-37 0.0 0.0 0.0 0.031052562433565432 0.0 38-39 0.0 0.0 0.0 0.05577517944798099 0.0 40-41 0.0 0.0 0.0 0.08384191857062667 0.0 42-43 0.0 0.0 0.0 0.11190865769327235 0.0 44-45 0.0 0.0 0.0 0.1452304458431368 0.0 46-47 0.0 0.0 0.0 0.18440444768240394 0.0 48-49 0.0 0.0 0.0 0.22751973629208516 0.0 50-51 0.0 0.0 0.0 0.2803090924291464 0.0 52-53 0.0 0.0 0.0 0.3336956132283916 0.0 54-55 0.0 0.0 0.0 0.3863655364330161 0.0 56-57 0.0 0.0 0.0 0.44321561227292816 0.0 58-59 0.0 0.0 0.0 0.5102174873699674 0.0 60-61 0.0 0.0 0.0 0.5892820886431225 0.0 62-63 0.0 0.0 0.0 0.665838598335105 0.0 64-65 0.0 0.0 0.0 0.7434700044190184 0.0 66-67 0.0 0.0 0.0 0.83196980735468 0.0 68-69 0.0 0.0 0.0 0.9375485196288025 0.0 70-71 0.0 0.0 0.0 1.0694024770390187 0.0 72-73 0.0 0.0 0.0 1.198987208732936 0.0 74-75 0.0 0.0 0.0 1.3234363243320713 0.0 76-77 0.0 0.0 0.0 1.45779837332346 0.0 78-79 0.0 0.0 0.0 1.6156887100048967 0.0 80-81 0.0 0.0 0.0 1.7999737247548637 0.0 82-83 0.0 0.0 0.0 2.004801203883959 0.0 84-85 0.0 0.0 0.0 2.2025821399992833 0.0 86-87 0.0 0.0 0.0 2.4078873508581258 0.0 88-89 0.0 0.0 0.0 2.6300326051905554 0.0 90-91 0.0 0.0 0.0 2.894934849335356 0.0 92-93 0.0 0.0 0.0 3.1727358501833294 0.0 94-95 0.0 0.0 0.0 3.4544781378017175 0.0 96-97 0.0 0.0 0.0 3.7290544494738977 0.0 98-99 0.0 0.0 0.0 4.026203585376631 0.0 100-101 0.0 0.0 0.0 4.371961924781139 0.0 102-103 0.0 0.0 0.0 4.760118955200707 0.0 104-105 0.0 0.0 0.0 5.15627799209354 0.0 106-107 0.0 0.0 0.0 5.521623332417681 0.0 108-109 0.0 0.0 0.0 5.905600210201961 0.0 110-111 0.0 0.0 0.0 6.335200468177096 0.0 112-113 0.0 0.0 0.0 6.812454466194509 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 35 0.007708159 51.141075 2 GTATCAA 1380 0.0 47.996838 1 GGTATCA 825 0.0 44.84422 1 ATCAACG 1575 0.0 39.743057 3 TCAACGC 1590 0.0 39.372837 4 CAACGCA 1635 0.0 38.639957 5 TATCAAC 1740 0.0 37.376095 2 AACGCAG 1655 0.0 37.09264 6 GTACATG 3305 0.0 36.651627 1 TACATGG 3275 0.0 35.889843 2 ACATGGG 3440 0.0 32.233467 3 GGCCCGC 130 1.7147449E-6 32.080956 9 GTGTAGG 245 0.0 31.662556 1 ACGCAGA 1955 0.0 31.095814 7 CGCAGAG 1975 0.0 30.78092 8 CATGGGG 1130 0.0 30.074675 4 AGGCCCG 140 3.0479277E-6 29.800156 8 ACGAATC 70 3.1014897E-6 29.739635 72-73 AAGCGTT 85 0.0071461927 28.047205 7 ATGGGAT 735 0.0 27.570213 5 >>END_MODULE