FastQCFastQC Report
Fri 27 May 2016
SRR940026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418645
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60211.4382113724038268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.964540362359517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30250.7225692412425803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.44572370385410076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.2625135854960647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.2457929749549141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.2371938038194652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.13710900643743507No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10127912670639802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.079.8839341
GTATCAA29900.060.7918621
TAACGCA902.3646862E-1159.5959554
ATAACGC1051.10958354E-1051.082253
TATAACG1152.6921043E-1046.6403162
ATCAACG39100.046.1830563
TATCAAC39700.045.4850772
TCAACGC39750.045.2779354
CAACGCA40500.044.5386475
AACGCAG42000.043.069136
TGGTATC5800.038.0181122
GTATAAC1601.6734703E-1037.2474751
ACGCAGA48750.037.2233247
CGCAGAG49300.036.8080528
GTGGTAT6300.035.946771
GTACATG29950.035.8172721
GCAGAGT51850.034.883079
TACATGG30750.034.3040122
ACATGGG31000.032.8738983
CTATAGC750.003876959231.7845121
Produced by FastQC (version 0.11.5)