##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418645 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.11305999116196 34.0 33.0 34.0 31.0 34.0 2 33.14221118131113 34.0 33.0 34.0 31.0 34.0 3 33.20760787779622 34.0 33.0 34.0 31.0 34.0 4 36.4457284811714 37.0 37.0 37.0 35.0 37.0 5 36.46648592482891 37.0 37.0 37.0 35.0 37.0 6 36.56245745201782 37.0 37.0 37.0 35.0 37.0 7 36.549326995425716 37.0 37.0 37.0 35.0 37.0 8 36.55666017747734 37.0 37.0 37.0 35.0 37.0 9 38.42018894289912 39.0 39.0 39.0 37.0 39.0 10-11 38.40841405009017 39.0 39.0 39.0 37.0 39.0 12-13 38.3888939316127 39.0 39.0 39.0 37.0 39.0 14-15 39.94364318217105 41.0 40.0 41.0 38.0 41.0 16-17 39.95948834931744 41.0 40.0 41.0 38.0 41.0 18-19 40.040732601607566 41.0 40.0 41.0 38.0 41.0 20-21 40.033033954782695 41.0 40.0 41.0 38.0 41.0 22-23 39.9565753800953 41.0 40.0 41.0 38.0 41.0 24-25 39.90708117856418 41.0 40.0 41.0 37.5 41.0 26-27 39.79113210476657 41.0 40.0 41.0 37.0 41.0 28-29 39.68310382304817 41.0 40.0 41.0 36.0 41.0 30-31 39.423162822916794 41.0 39.0 41.0 35.5 41.0 32-33 39.1376512319507 41.0 39.0 41.0 35.0 41.0 34-35 38.96925796319077 41.0 39.0 41.0 35.0 41.0 36-37 38.82131400112267 41.0 38.5 41.0 35.0 41.0 38-39 38.67855820563962 41.0 38.0 41.0 35.0 41.0 40-41 38.50646848762078 41.0 38.0 41.0 35.0 41.0 42-43 38.2826105650372 41.0 38.0 41.0 35.0 41.0 44-45 37.96694335296014 41.0 38.0 41.0 34.5 41.0 46-47 37.488672980687696 41.0 38.0 41.0 33.0 41.0 48-49 37.05861529458133 40.5 37.0 41.0 32.5 41.0 50-51 37.02929092668012 41.0 37.0 41.0 33.0 41.0 52-53 36.91411100096741 41.0 37.0 41.0 32.0 41.0 54-55 36.75009136619332 40.5 36.5 41.0 32.0 41.0 56-57 36.55561872230649 40.0 36.0 41.0 31.5 41.0 58-59 36.336485566530115 40.0 35.0 41.0 31.0 41.0 60-61 36.10491705382842 39.0 35.0 41.0 31.0 41.0 62-63 35.82802135460832 39.0 35.0 41.0 31.0 41.0 64-65 35.52623224928041 38.5 35.0 41.0 30.5 41.0 66-67 35.20125762877856 37.0 35.0 41.0 30.0 41.0 68-69 34.859339058151896 37.0 35.0 40.0 29.5 41.0 70-71 34.49221416713445 36.5 35.0 39.0 29.0 41.0 72-73 34.13648795518876 36.0 35.0 39.0 29.0 41.0 74-75 33.792509166477565 35.5 35.0 38.5 29.0 40.5 76-77 33.438796593772764 35.0 35.0 37.0 28.5 39.5 78-79 33.12104408269536 35.0 35.0 37.0 27.5 39.0 80-81 32.8254571295489 35.0 35.0 36.5 27.0 39.0 82-83 32.56855569754804 35.0 35.0 36.0 27.0 37.0 84-85 32.33632552640065 35.0 34.5 36.0 26.0 37.0 86-87 32.14833928507447 35.0 34.0 35.0 26.0 37.0 88-89 32.043454478137804 35.0 34.5 35.0 25.5 36.0 90-91 32.02895293148133 35.0 35.0 35.0 26.0 36.0 92-93 31.936468845919574 35.0 35.0 35.0 26.0 36.0 94-95 31.85403862461035 35.0 35.0 35.0 25.5 36.0 96-97 31.78159538511149 35.0 35.0 35.0 25.0 36.0 98-99 31.719131961447047 35.0 35.0 35.0 25.0 35.0 100-101 31.66020375258274 35.0 34.0 35.0 25.0 35.0 102-103 31.1875264245363 35.0 33.5 35.0 23.5 35.0 104-105 31.55053087938468 35.0 34.0 35.0 24.0 35.0 106-107 31.574653943078264 35.0 34.0 35.0 24.0 35.0 108-109 31.550023289421826 35.0 34.5 35.0 24.0 35.0 110-111 31.500268724097985 35.0 34.0 35.0 24.0 35.0 112-113 31.46321226815082 35.0 34.0 35.0 23.0 35.0 114-115 31.394097624478974 35.0 34.0 35.0 21.0 35.0 116-117 31.326140285922442 35.0 34.0 35.0 19.0 35.0 118-119 31.256227830261917 35.0 34.0 35.0 18.5 35.0 120-121 31.162175590297267 35.0 34.0 35.0 8.5 35.0 122-123 31.055906555673662 35.0 34.0 35.0 2.0 35.0 124-125 30.060630128151537 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06268427503321305 1101 2 0.02889648057389138 1101 3 0.0461494557080826 1101 4 -0.01582679411676935 1101 5 -0.023926806402286616 1101 6 0.029048898047996374 1101 7 0.061185487982832854 1101 8 0.09202106970914059 1101 9 0.07453764655313933 1101 10-11 0.06736447998471107 1101 12-13 0.07524696870104464 1101 14-15 0.026448402005208038 1101 16-17 0.07139100654129038 1101 18-19 0.03755761203942143 1101 20-21 -0.016855297015005988 1101 22-23 0.08585703036789738 1101 24-25 0.10853347937358393 1101 26-27 -0.018433800571052927 1101 28-29 0.0423003266099613 1101 30-31 -0.005931516638327139 1101 32-33 -0.059865141443602 1101 34-35 -0.04655511074567187 1101 36-37 -0.08330263667902216 1101 38-39 -0.015267793174459143 1101 40-41 -0.2668770995618246 1101 42-43 -0.21001444116361512 1101 44-45 -0.36753977938118965 1101 46-47 -0.1607771376931737 1101 48-49 -0.26325974650098516 1101 50-51 -0.34667597669969297 1101 52-53 -0.40513235578909956 1101 54-55 -0.534697347611413 1101 56-57 -0.4338372529586394 1101 58-59 -0.3874587830141607 1101 60-61 -0.35652761960619017 1101 62-63 -0.5358172232532681 1101 64-65 -0.5054867757841137 1101 66-67 -0.579028782170063 1101 68-69 -0.6596740441805906 1101 70-71 -0.6301334238530103 1101 72-73 -0.5681911310595069 1101 74-75 -0.6055519408675778 1101 76-77 -0.600284205133633 1101 78-79 -0.496911005000797 1101 80-81 -0.44448765249898514 1101 82-83 -0.4961951873107182 1101 84-85 -0.5824698353679629 1101 86-87 -0.5383771630295797 1101 88-89 -0.6597561154957532 1101 90-91 -0.806060149452307 1101 92-93 -0.8905391663176232 1101 94-95 -0.8125239623739233 1101 96-97 -0.8037221042417215 1101 98-99 -0.7707244893676517 1101 100-101 -0.8050890737273235 1101 102-103 -0.7063344183270814 1101 104-105 -0.7352751389973449 1101 106-107 -0.7553439472884591 1101 108-109 -0.7280565602553501 1101 110-111 -0.9132309020589773 1101 112-113 -0.8435771196503694 1101 114-115 -0.7698920924072432 1101 116-117 -0.610950392053482 1101 118-119 -0.6452617942454566 1101 120-121 -0.6903429422960485 1101 122-123 -0.6881747710836912 1101 124-125 -0.5246397878424567 1102 1 0.13055915390918216 1102 2 0.1454783604188492 1102 3 0.16538092373134106 1102 4 0.08129953824757052 1102 5 0.08885480696980608 1102 6 0.0824797362262899 1102 7 0.10304898119989048 1102 8 0.06590370052309424 1102 9 0.049131881049262915 1102 10-11 0.10246773822114363 1102 12-13 0.024367305425847974 1102 14-15 0.19723328572613497 1102 16-17 0.18012253512655718 1102 18-19 0.2023847074851588 1102 20-21 0.2802846618028241 1102 22-23 0.17320259722836084 1102 24-25 0.22495638392784656 1102 26-27 0.2816131349909625 1102 28-29 0.231594476319259 1102 30-31 0.24535542619112505 1102 32-33 0.19575198549438255 1102 34-35 0.41002192413805005 1102 36-37 0.40707557310780373 1102 38-39 0.5525465236860043 1102 40-41 0.6023316577056193 1102 42-43 0.5319459512782458 1102 44-45 0.5220253853474901 1102 46-47 0.5343915275200004 1102 48-49 0.36124818712304574 1102 50-51 0.4945834104127087 1102 52-53 0.5343813312264061 1102 54-55 0.5733301475436292 1102 56-57 0.622780661285546 1102 58-59 0.4198253134005725 1102 60-61 0.5449152704131919 1102 62-63 0.5543974690916897 1102 64-65 0.5250667610375856 1102 66-67 0.5832471383725775 1102 68-69 0.7307389887651397 1102 70-71 0.5689793426198548 1102 72-73 0.520221841324215 1102 74-75 0.39774868897738 1102 76-77 0.44036047752140917 1102 78-79 0.465941768739512 1102 80-81 0.5125587380048913 1102 82-83 0.40279494108075653 1102 84-85 0.40274546259715294 1102 86-87 0.6829997143928992 1102 88-89 0.6136965250081232 1102 90-91 0.7230398989973068 1102 92-93 0.5795734790312146 1102 94-95 0.7815742692152234 1102 96-97 0.7303364592272708 1102 98-99 0.7044148032680084 1102 100-101 0.7973061539858577 1102 102-103 0.7165777061884242 1102 104-105 0.7603871066421917 1102 106-107 0.7237167358510774 1102 108-109 0.7551829019539511 1102 110-111 0.7654270436774553 1102 112-113 0.7826568314155224 1102 114-115 0.8837234599183397 1102 116-117 0.9105188424426416 1102 118-119 0.7737213695142344 1102 120-121 0.9240057132272064 1102 122-123 0.9255682642845251 1102 124-125 1.0375801128358368 1103 1 0.07812183214112167 1103 2 0.17200521403101732 1103 3 0.15714512287582494 1103 4 -0.1229022797182111 1103 5 0.011509571703648191 1103 6 0.048266630237698394 1103 7 0.13326672121890226 1103 8 0.15177436354591123 1103 9 0.14301555500363605 1103 10-11 0.18061130404813497 1103 12-13 0.19906059796387154 1103 14-15 0.25105933135350966 1103 16-17 0.2606945111246546 1103 18-19 0.1535677219813536 1103 20-21 0.11177750281993326 1103 22-23 0.2283431034070702 1103 24-25 0.19071728578146008 1103 26-27 0.15017821935408193 1103 28-29 0.2917366162907413 1103 30-31 0.23048048560177392 1103 32-33 0.23112359908753888 1103 34-35 0.37375172451827865 1103 36-37 0.34505457144430807 1103 38-39 0.39575440153771524 1103 40-41 0.3090027306618879 1103 42-43 0.33949258863566456 1103 44-45 0.4553814927619442 1103 46-47 0.7022154640694254 1103 48-49 0.8848007952504204 1103 50-51 0.8000692409735422 1103 52-53 0.8466214096484563 1103 54-55 0.9652547554333637 1103 56-57 0.8594689633292774 1103 58-59 1.0040361914899236 1103 60-61 0.9256075529702272 1103 62-63 0.9300101413825601 1103 64-65 0.8141738190223791 1103 66-67 0.5769347571938752 1103 68-69 0.6459748490312975 1103 70-71 0.7441739124867723 1103 72-73 0.7489788220751663 1103 74-75 0.7637925340344154 1103 76-77 0.5246678979111437 1103 78-79 0.568295172969556 1103 80-81 0.5901713211797954 1103 82-83 0.5328782645123127 1103 84-85 0.530979629659889 1103 86-87 0.5274737816457531 1103 88-89 0.4880207289054681 1103 90-91 0.46057851591745447 1103 92-93 0.5220194153429993 1103 94-95 0.4641951698806217 1103 96-97 0.42851180975008774 1103 98-99 0.41382247912352454 1103 100-101 0.48946246339650656 1103 102-103 0.5634801836123771 1103 104-105 0.518151335492 1103 106-107 0.5722270138929062 1103 108-109 0.6397257370299982 1103 110-111 0.6425064163010248 1103 112-113 0.6467661172995491 1103 114-115 0.6243514304506554 1103 116-117 0.624827896625149 1103 118-119 0.6307867806359084 1103 120-121 0.6497401773431761 1103 122-123 0.6685912264993554 1103 124-125 0.6314600736248117 1104 1 0.21121985539101473 1104 2 0.1852877533442765 1104 3 0.18031504777531637 1104 4 0.1848898525401168 1104 5 0.06010316380537262 1104 6 0.1511826819719886 1104 7 0.15437919750008433 1104 8 0.11190857026496559 1104 9 0.21701100389821448 1104 10-11 0.2328472345165551 1104 12-13 0.20639071305013346 1104 14-15 0.28859322836476053 1104 16-17 0.20561959236842853 1104 18-19 0.2905498156352522 1104 20-21 0.24655311005808755 1104 22-23 0.18167609746736701 1104 24-25 0.13493898132268356 1104 26-27 0.10591217598044977 1104 28-29 0.11570843724182112 1104 30-31 0.13670117786416824 1104 32-33 0.17501450341980984 1104 34-35 0.29074312394684654 1104 36-37 0.23050448383915523 1104 38-39 0.05497766015827921 1104 40-41 0.14839814611384128 1104 42-43 0.15868247732986873 1104 44-45 0.049667892517661016 1104 46-47 0.03808304580394406 1104 48-49 -0.07527525037695426 1104 50-51 -0.2095218865519186 1104 52-53 -0.34787473354415255 1104 54-55 -0.3328190219114333 1104 56-57 -0.2280144062335765 1104 58-59 -0.2951099309827967 1104 60-61 -0.18409517114990592 1104 62-63 -0.3466821646320213 1104 64-65 -0.26218078915361787 1104 66-67 -0.21291831334826128 1104 68-69 -0.1588328922100004 1104 70-71 -0.13019085933999008 1104 72-73 -0.01268065927330042 1104 74-75 -0.05719126083141646 1104 76-77 -0.038702246546463925 1104 78-79 -0.024331818139074812 1104 80-81 -0.00976215707159156 1104 82-83 -0.06119548375193773 1104 84-85 -0.07697826170972633 1104 86-87 0.02261006035369917 1104 88-89 0.039787735572176075 1104 90-91 0.07429617289788126 1104 92-93 0.12983190398341193 1104 94-95 0.04347600798195472 1104 96-97 0.14486016560872272 1104 98-99 0.20805217181485247 1104 100-101 0.1308648000183652 1104 102-103 0.03830370104979863 1104 104-105 -0.01584564013123213 1104 106-107 0.13808172748587566 1104 108-109 0.06069571863655199 1104 110-111 0.18078913736770374 1104 112-113 0.1179800119843577 1104 114-115 0.09026176058755553 1104 116-117 0.0406051832648231 1104 118-119 -0.10875876313394883 1104 120-121 -0.21822323933015397 1104 122-123 -0.2723789005099313 1104 124-125 -0.4522191970351024 1105 1 0.09281438149698573 1105 2 0.113347468551936 1105 3 0.10984502377755234 1105 4 -0.06352413897054277 1105 5 -0.02374411285459388 1105 6 0.04618626926695413 1105 7 0.053397727734086686 1105 8 0.04687427007022649 1105 9 0.158730714228561 1105 10-11 0.0759958545566306 1105 12-13 0.14514516182602932 1105 14-15 0.08442136336014272 1105 16-17 0.1562969372753571 1105 18-19 0.2761034430443132 1105 20-21 0.18120779315680124 1105 22-23 0.15909027629490424 1105 24-25 0.07031112341491053 1105 26-27 0.04344199647432845 1105 28-29 0.12503363701704018 1105 30-31 0.05623444074750239 1105 32-33 0.12433115023560504 1105 34-35 0.058719359443038854 1105 36-37 0.24995009655974343 1105 38-39 0.3377713677248195 1105 40-41 0.3293003066196718 1105 42-43 0.32899063126900074 1105 44-45 0.37301747176154265 1105 46-47 0.48078528618451344 1105 48-49 0.5189946202330233 1105 50-51 0.48783411801436927 1105 52-53 0.508557480718089 1105 54-55 0.5788646034123914 1105 56-57 0.5697628845988518 1105 58-59 0.5322563583404971 1105 60-61 0.6896974301867616 1105 62-63 0.6851864449465879 1105 64-65 0.6758508992076386 1105 66-67 0.631400153622117 1105 68-69 0.7015612507799247 1105 70-71 0.5232198983037719 1105 72-73 0.5738208622345695 1105 74-75 0.6563482153174931 1105 76-77 0.6147102782376805 1105 78-79 0.6370929211193612 1105 80-81 0.6111067105095103 1105 82-83 0.7676339978044098 1105 84-85 0.6433823762986322 1105 86-87 0.6313899206082354 1105 88-89 0.6712541440007271 1105 90-91 0.6382648874446275 1105 92-93 0.6238130238556074 1105 94-95 0.5920706012392536 1105 96-97 0.6489351913206143 1105 98-99 0.6376475320110728 1105 100-101 0.7190981190967634 1105 102-103 0.7748230706754882 1105 104-105 0.6981921696042974 1105 106-107 0.6439274278566245 1105 108-109 0.623392092111068 1105 110-111 0.5822304170600816 1105 112-113 0.6352608777417714 1105 114-115 0.6948587995671396 1105 116-117 0.8369210490045589 1105 118-119 0.8977213406325291 1105 120-121 0.877511590653274 1105 122-123 0.9294727091782455 1105 124-125 0.8141242007517313 1106 1 0.058033112242512175 1106 2 0.11197141597440208 1106 3 0.1585956228054144 1106 4 -0.09058992934502186 1106 5 0.01595260789573416 1106 6 0.038049759374438 1106 7 0.03599168953322618 1106 8 0.07763691811568663 1106 9 0.08203118660481579 1106 10-11 0.13062890720262743 1106 12-13 0.1267327568147394 1106 14-15 0.03357587831872877 1106 16-17 0.09079238466359385 1106 18-19 0.08320791350367784 1106 20-21 0.21404478911763647 1106 22-23 0.22599484259873037 1106 24-25 0.17954984457599465 1106 26-27 0.18296585489837014 1106 28-29 0.09320471937481045 1106 30-31 0.06730276415408554 1106 32-33 0.11280927716104827 1106 34-35 0.21436220191583288 1106 36-37 0.04273095505224944 1106 38-39 -0.01309294390658522 1106 40-41 0.09477234057598594 1106 42-43 0.26720347442639536 1106 44-45 0.37923024645860437 1106 46-47 0.4289300113162966 1106 48-49 0.5969469718452629 1106 50-51 0.7193446951349287 1106 52-53 0.6547082988189956 1106 54-55 0.6211166058769635 1106 56-57 0.5505512052670269 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0.12443513409680662 2216 110-111 0.08589898245296723 2216 112-113 0.07449755207878894 2216 114-115 0.009664148693850905 2216 116-117 -0.2365634279655211 2216 118-119 -0.1317394085469914 2216 120-121 -0.0922769717217733 2216 122-123 -0.13707921658282274 2216 124-125 -0.09695033997028446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 9.0 5 44.0 6 85.0 7 207.0 8 304.0 9 509.0 10 1118.0 11 1577.0 12 1887.0 13 2925.0 14 5536.0 15 6006.0 16 2438.0 17 1831.0 18 1719.0 19 1400.0 20 1187.0 21 1117.0 22 1133.0 23 1156.0 24 1303.0 25 1582.0 26 1802.0 27 2191.0 28 2545.0 29 3120.0 30 3670.0 31 4635.0 32 5843.0 33 7636.0 34 11487.0 35 26314.0 36 51022.0 37 158828.0 38 103511.0 39 962.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.85687254517828 18.02997868904158 13.687751454974627 21.425397310805515 2 15.435752641938505 20.60497047526228 41.358281258957746 22.600995623841463 3 19.035266240528944 27.63254698502785 29.264960204851853 24.067226569591345 4 11.99950243281647 16.753661185453815 37.07186496792127 34.17497141380845 5 12.50761697887319 38.62662585160811 32.778458825300554 16.087298344218144 6 29.773204545943223 37.51454353024935 17.272577412195304 15.439674511612129 7 26.990409535525327 34.67090255467043 20.513322743613323 17.825365166190927 8 27.020267768634522 33.86258046793823 19.426005326708786 19.69114643671846 9 28.226062654516355 15.351192537830382 19.88868850696891 36.534056300684355 10-11 24.97700916050592 27.32971849657825 26.05298044882896 21.640291894086875 12-13 26.899998805670677 24.93974608558564 27.554013543694538 20.606241565049146 14-15 23.149446428358157 24.704343775752726 27.931660476059665 24.21454931982945 16-17 20.880937309653763 28.374040057805537 28.051332274361336 22.693690358179364 18-19 20.74490319960826 24.917173261355085 33.41040738573254 20.92751615330411 20-21 21.958222360233613 23.892080402250116 33.81134373992285 20.338353497593424 22-23 21.552747554610708 24.08544231986528 33.78375473252995 20.578055392994063 24-25 20.97791685079244 24.313439787887113 33.93507625792736 20.77356710339309 26-27 20.579369155250866 24.567831933977473 33.87500149291166 20.97779741786 28-29 20.6482819572669 24.568190232774786 33.89590225608809 20.887625553870222 30-31 20.790407146866677 24.27928196921019 33.98272999796964 20.947580885953492 32-33 20.628456090482388 24.34258142340169 33.75306046889369 21.27590201722223 34-35 21.039330505954336 24.580998914353348 33.48031563773082 20.899354941961498 36-37 21.212609804307974 24.326125512818216 33.23050096442669 21.230763718447125 38-39 20.97094939105156 24.478210550328868 33.247736971537016 21.303103087082555 40-41 21.147272748987806 24.299107835994697 33.241409786334486 21.312209628683014 42-43 21.183279727403995 24.414086993286617 32.80061819002655 21.602015089282837 44-45 21.130055918766107 24.72357115728676 32.53953846117098 21.60683446277616 46-47 21.639455863559817 25.0811546775908 31.6377838025057 21.64160565634368 48-49 21.79894634882757 25.3660966057909 30.903916137870414 21.931040907511115 50-51 21.845932246305843 25.67959124199181 30.54040044525767 21.934076066444675 52-53 21.997157496208004 25.77052156361595 30.3707198222838 21.86160111789225 54-55 21.66704487095272 25.809337266657906 30.524191140465074 21.999426721924305 56-57 21.757450604994023 25.91173094815241 30.385258951295924 21.945559495557646 58-59 21.79788217477194 25.419874497476336 30.597749362813133 22.184493964938596 60-61 21.761630802562102 25.4176315956053 30.953646386338633 21.86709121549396 62-63 21.659042864479453 25.48722664787589 31.04145517084881 21.812275316795855 64-65 21.509146869450042 25.519747850482695 30.946599767581016 22.02450551248625 66-67 21.63382643445609 26.053463213286737 30.436911640705805 21.87579871155137 68-69 21.797382966830845 25.61866702337558 30.683495683662894 21.90045432613068 70-71 21.812935411324485 25.528405568965333 30.49303521031718 22.165623809393 72-73 21.755305808023504 25.77267135639981 30.341817052634095 22.13020578294259 74-75 21.726548419960135 26.218306940614294 29.944260583860533 22.110884055565045 76-77 21.946931404492773 25.935795025655235 30.136780148485055 21.980493421366944 78-79 21.758447551846206 26.122080441045092 30.05133264220981 22.068139364898887 80-81 21.67659950554766 26.311194448757302 29.97587454764777 22.03633149804727 82-83 21.912957278840068 25.915871442391524 29.922368593916087 22.248802684852322 84-85 21.709588923298885 26.060177549209413 29.762005711289653 22.468227816202052 86-87 21.78065160297102 26.052533832404347 29.841667572367488 22.325146992257153 88-89 21.723536647995317 26.12631226934515 29.595361224904153 22.554789857755377 90-91 21.79376321226815 26.177907296157844 29.736530951044443 22.291798540529566 92-93 21.732291552209286 25.72448535980623 29.91876113720076 22.624461950783726 94-95 21.642409606321404 25.846608430612388 29.883718985956943 22.627262977109268 96-97 22.00237194653685 25.63147403459029 29.73842899746443 22.62772502140843 98-99 21.683168316831683 25.60128509835302 29.691385302583335 23.02416128223196 100-101 21.456655296624337 25.780615511030852 29.895089861553014 22.867639330791796 102-103 21.789463626700424 25.466803616429196 29.75970093993718 22.9840318169332 104-105 21.73918236214454 25.41234219923802 29.705000656881133 23.143474781736316 106-107 21.685463442132882 25.60477923393237 29.56912129503672 23.140636028898026 108-109 21.868819330982888 25.321155922454402 29.484836758678018 23.325187987884693 110-111 21.699769494440396 25.585758817136238 29.592733700390543 23.12173798803282 112-113 21.604368384154096 25.716329726056358 29.466647716618755 23.212654173170794 114-115 21.432145949658118 25.77528291182706 29.3997790451166 23.392792093398228 116-117 21.350001433198614 25.54246567489323 29.548494663723833 23.559038228184328 118-119 21.479253828137786 25.809429741534263 29.1796003043162 23.53171612601175 120-121 21.15709151798244 25.899420630152793 29.367518264303005 23.57596958756176 122-123 21.37938200475822 25.879745081740097 29.15794804177296 23.582924871728725 124-125 21.359274321169018 25.767928483132984 29.120908651176126 23.751888544521876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 525.0 1 367.0 2 990.0 3 1440.0 4 654.0 5 280.0 6 727.0 7 1974.5 8 3480.5 9 3568.5 10 2138.5 11 1006.5 12 738.0 13 740.5 14 793.0 15 859.5 16 917.0 17 971.5 18 1015.0 19 1059.5 20 1158.5 21 1262.5 22 1379.0 23 1615.5 24 1945.0 25 2216.5 26 2557.5 27 2935.0 28 3286.5 29 3885.0 30 4686.0 31 5447.5 32 6430.5 33 7091.0 34 7666.0 35 8113.0 36 8779.5 37 9948.0 38 10482.5 39 10512.5 40 10490.0 41 10957.5 42 11399.5 43 11833.5 44 12661.5 45 13556.5 46 13868.5 47 14027.5 48 14563.5 49 14642.0 50 14617.5 51 14559.5 52 14171.5 53 14457.0 54 14624.5 55 13781.5 56 12475.0 57 11049.0 58 10181.5 59 9271.0 60 8050.0 61 6835.0 62 5567.5 63 4131.5 64 2971.5 65 2307.5 66 1673.5 67 1320.0 68 985.0 69 614.5 70 420.0 71 297.5 72 231.0 73 196.0 74 154.5 75 117.5 76 81.0 77 51.5 78 36.0 79 16.0 80 8.5 81 4.5 82 3.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01934813505476 2 0.003105256243356543 3 2.3886586487358022E-4 4 0.14594704343775752 5 0.0410849287582558 6 0.01767607400064494 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.1943293243679011E-4 36-37 5.971646621839506E-4 38-39 0.0032246891757933337 40-41 0.0 42-43 8.36030527057531E-4 44-45 4.7773172974716044E-4 46-47 0.0 48-49 0.0013137622568046912 50-51 0.0026275245136093825 52-53 0.0 54-55 0.0 56-57 0.0010748963919311111 58-59 0.0019109269189886418 60-61 0.0010748963919311111 62-63 0.0 64-65 0.0010748963919311111 66-67 4.7773172974716044E-4 68-69 7.165975946207407E-4 70-71 8.36030527057531E-4 72-73 0.0 74-75 1.1943293243679011E-4 76-77 0.004060719702850864 78-79 7.165975946207407E-4 80-81 0.0 82-83 0.0 84-85 1.1943293243679011E-4 86-87 1.1943293243679011E-4 88-89 0.0 90-91 0.0 92-93 7.165975946207407E-4 94-95 9.554634594943209E-4 96-97 3.5829879731037035E-4 98-99 0.0 100-101 2.3886586487358022E-4 102-103 0.0 104-105 0.0 106-107 1.1943293243679011E-4 108-109 2.3886586487358022E-4 110-111 0.0 112-113 1.1943293243679011E-4 114-115 0.0017914939865518516 116-117 2.3886586487358022E-4 118-119 3.5829879731037035E-4 120-121 1.1943293243679011E-4 122-123 2.3886586487358022E-4 124-125 5.971646621839506E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 418645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.091009666471386 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43595207009278 49.139135254458665 2 11.62596261502812 14.204835889934357 3 3.4589662063429234 6.339352138430801 4 1.4783191804630136 3.6124804537518407 5 0.7595393487305061 2.320051284768036 6 0.4908612122801216 1.7992324238580462 7 0.3373892626882726 1.4428015495777038 8 0.23593029405231222 1.1530575899650592 9 0.176668751329644 0.9713585155718438 >10 0.9268791943978061 9.976076784205242 >50 0.04654410578508974 1.9153702649428357 >100 0.023858743295309806 2.6711482269694597 >500 7.822538785347477E-4 0.37442430196449983 >1k 0.001955634696336869 2.6419971326237874 >5k 3.9112693926737383E-4 1.4386781889778262 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6021 1.4382113724038268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.964540362359517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3025 0.7225692412425803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 0.44572370385410076 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1099 0.2625135854960647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.2457929749549141 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 993 0.2371938038194652 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 574 0.13710900643743507 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.10127912670639802 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1943293243679012E-4 0.0 16-17 0.0 0.0 0.0 2.3886586487358025E-4 0.0 18-19 0.0 0.0 0.0 2.3886586487358025E-4 0.0 20-21 0.0 0.0 0.0 4.777317297471605E-4 0.0 22-23 0.0 0.0 0.0 5.971646621839505E-4 0.0 24-25 0.0 0.0 0.0 0.0011943293243679013 0.0 26-27 0.0 0.0 0.0 0.0015526281216782715 0.0 28-29 0.0 0.0 0.0 0.002269225716299012 0.0 30-31 0.0 0.0 0.0 0.005255049027218765 0.0 32-33 0.0 0.0 0.0 0.011346128581495061 0.0 34-35 0.0 0.0 0.0 0.018870403325012837 0.0 36-37 0.0 0.0 0.0 0.032008025893059754 0.0 38-39 0.0 0.0 0.0 0.05625291117772815 0.0 40-41 0.0 0.0 0.0 0.08420021736793704 0.0 42-43 0.0 0.0 0.0 0.11190865769327235 0.0 44-45 0.0 0.0 0.0 0.1448721470458264 0.0 46-47 0.0 0.0 0.0 0.18404614888509357 0.0 48-49 0.0 0.0 0.0 0.22811690095426912 0.0 50-51 0.0 0.0 0.0 0.28126455588864074 0.0 52-53 0.0 0.0 0.0 0.33453164375544914 0.0 54-55 0.0 0.0 0.0 0.3879181645546943 0.0 56-57 0.0 0.0 0.0 0.4446488074621696 0.0 58-59 0.0 0.0 0.0 0.5115312496267721 0.0 60-61 0.0 0.0 0.0 0.5903569850350536 0.0 62-63 0.0 0.0 0.0 0.666316330064852 0.0 64-65 0.0 0.0 0.0 0.74490319960826 0.0 66-67 0.0 0.0 0.0 0.834239033070979 0.0 68-69 0.0 0.0 0.0 0.9404149100072854 0.0 70-71 0.0 0.0 0.0 1.072627166214812 0.0 72-73 0.0 0.0 0.0 1.203047928435787 0.0 74-75 0.0 0.0 0.0 1.328213641629543 0.0 76-77 0.0 0.0 0.0 1.4620979588911847 0.0 78-79 0.0 0.0 0.0 1.6213020578294257 0.0 80-81 0.0 0.0 0.0 1.8065425360388874 0.0 82-83 0.0 0.0 0.0 2.0141169726140284 0.0 84-85 0.0 0.0 0.0 2.2123756404591 0.0 86-87 0.0 0.0 0.0 2.4193529123720574 0.0 88-89 0.0 0.0 0.0 2.6430507948261655 0.0 90-91 0.0 0.0 0.0 2.9133275209306215 0.0 92-93 0.0 0.0 0.0 3.194950375616573 0.0 94-95 0.0 0.0 0.0 3.478006425491765 0.0 96-97 0.0 0.0 0.0 3.754613097015371 0.0 98-99 0.0 0.0 0.0 4.05677841608045 0.0 100-101 0.0 0.0 0.0 4.406716908120245 0.0 102-103 0.0 0.0 0.0 4.799292957039974 0.0 104-105 0.0 0.0 0.0 5.20130420762221 0.0 106-107 0.0 0.0 0.0 5.573098926297938 0.0 108-109 0.0 0.0 0.0 5.961494822582379 0.0 110-111 0.0 0.0 0.0 6.396111263719858 0.0 112-113 0.0 0.0 0.0 6.878500877832053 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1410 0.0 79.883934 1 GTATCAA 2990 0.0 60.791862 1 TAACGCA 90 2.3646862E-11 59.595955 4 ATAACGC 105 1.10958354E-10 51.08225 3 TATAACG 115 2.6921043E-10 46.640316 2 ATCAACG 3910 0.0 46.183056 3 TATCAAC 3970 0.0 45.485077 2 TCAACGC 3975 0.0 45.277935 4 CAACGCA 4050 0.0 44.538647 5 AACGCAG 4200 0.0 43.06913 6 TGGTATC 580 0.0 38.018112 2 GTATAAC 160 1.6734703E-10 37.247475 1 ACGCAGA 4875 0.0 37.223324 7 CGCAGAG 4930 0.0 36.808052 8 GTGGTAT 630 0.0 35.94677 1 GTACATG 2995 0.0 35.817272 1 GCAGAGT 5185 0.0 34.88307 9 TACATGG 3075 0.0 34.304012 2 ACATGGG 3100 0.0 32.873898 3 CTATAGC 75 0.0038769592 31.784512 1 >>END_MODULE