FastQCFastQC Report
Fri 27 May 2016
SRR940025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202689
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20530.1707008212430645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.043.6202161
GGTATCA17750.042.6894841
TATTCCG1652.3283064E-1036.109055
GTACATG62800.035.532641
ATCAACG29200.035.1125833
TCAACGC29300.034.585854
TACATGG64250.034.2692832
CAACGCA30650.032.8515785
TATCAAC31950.032.4960172
AACGCAG31350.031.9280036
ACATGGG67750.031.6745433
CATGGGG39700.030.180244
GTAAGGT4450.026.790894
GTACTAG1358.0931066E-526.5175271
ACGCAGA38200.026.3586647
CGCAGAG39250.025.3499368
GCGTAGA1200.001277858424.8249726
ATGGGGA24050.024.030165
TAGACCG1250.001620853123.8319765
TAAGGTG5000.023.8319765
Produced by FastQC (version 0.11.5)