FastQCFastQC Report
Fri 27 May 2016
SRR940025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202689
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.45198717207856726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.3216957999948449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27370.2275733793191756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.057.2247051
GTATCAA47700.057.1184231
ATCAACG62750.042.93693
TCAACGC63000.042.5772824
TATCAAC64500.042.0509452
CAACGCA65100.040.966095
AACGCAG66750.040.024316
GTACATG62650.036.7320021
TACATGG63550.035.5506672
ACGCAGA76450.034.7081957
CGCAGAG78000.034.018488
GTGTACG1451.0789154E-732.8927651
ACATGGG66150.032.890393
TATTCCG1858.039933E-1032.1777535
GCAGAGT84650.030.9946169
CATGGGG37200.029.9643364
GTAAGGT3550.028.5447714
AGTACTT51050.027.96984912-13
CATGGGA30150.026.6902374
GAGTACT51250.026.46766512-13
Produced by FastQC (version 0.11.5)