FastQCFastQC Report
Fri 27 May 2016
SRR940024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706472
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18900.267526526175135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18300.053.4688031
GGTATCA11000.050.442661
TCAACGC20600.043.7058834
ATCAACG21000.042.8733943
CAACGCA21400.042.051135
TATCAAC24100.040.3589632
AACGCAG22150.040.3582236
GTACATG47350.035.029391
CGCAGAG25850.034.5816088
ACGCAGA25950.034.218697
TACATGG47400.033.486732
ACATGGG48150.030.958073
GCAGAGT29650.028.5294449
CATGGGG14000.027.2572564
CATGGGA36450.027.1544344
GAGTACA35200.025.9332241
AGTACAT34300.025.5736452
ATGGGAT16500.023.4770685
GTAGCCC4450.022.7781853
GTGTAGC4750.022.6092741
Produced by FastQC (version 0.11.5)