FastQCFastQC Report
Fri 27 May 2016
SRR940024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706472
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62260.881280503685921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44700.6327214666681765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.43271353995628986No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.17099049926961013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.14522868563793043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.11380493494434316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.073.357161
GTATCAA44700.062.838621
TAACGCA1100.059.619734
TTCGCGA150.00423868959.49313448-49
ATAACGC1300.055.0336043
TATAACG1450.049.340472
ATCAACG57400.049.0252883
TCAACGC57750.048.83144
TATCAAC58450.048.042592
CAACGCA58850.047.9485475
AACGCAG61150.046.0346956
GTGGTAT9000.042.4082871
CGCAGAG71150.039.8822448
ACGCAGA71150.039.7150237
TGGTATC9100.039.3097152
GCAGAGT75000.037.3590479
TATCACG650.001919355236.6890682
GTACATG44600.032.7600481
ACATGGG44300.032.1650473
TACATGG44850.032.036472
Produced by FastQC (version 0.11.5)