FastQCFastQC Report
Fri 27 May 2016
SRR940023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751951
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.048.330031
ATCAACG28550.042.3798833
GGTATCA16500.041.948491
TCAACGC28900.041.8702284
CAACGCA29450.041.263525
AACGCAG31700.038.14686
TATCAAC32700.037.9464232
GTACATG66650.033.1240841
TACATGG68250.031.9040242
ACGCAGA38950.030.740437
GTACGGT800.005332597529.7845736
CGCAGAG40650.029.4548558
ACATGGG73200.029.068793
CATGGGG44750.024.5093564
GCAGAGT49100.023.527749
GAGTACA51750.023.5213411
AGTACAT49600.023.4535262
TATCCCG1300.002038772522.9112115
GGACCGA4102.3646862E-1120.3406856
AGAGTAC45950.020.00549310-11
Produced by FastQC (version 0.11.5)