FastQCFastQC Report
Fri 27 May 2016
SRR940023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751951
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.2430433271250166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29040.1657580605850278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.10639566974190487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.066.449971
GTATCAA49550.058.723471
ATCAACG58650.048.7861983
TCAACGC59400.048.1702084
CAACGCA60350.047.3415455
TATCAAC61800.046.4937632
AACGCAG63500.044.975136
ACGCAGA76350.036.935967
CGCAGAG77050.036.4459658
GCAGAGT88000.031.9785359
GTACATG65900.030.8535021
TACATGG65800.030.5309412
ACATGGG68650.028.2206213
GAGTACT53900.026.98796712-13
CATGGGG44800.026.611894
GTGGTAT12250.025.7973611
CAGAGTA87700.025.47360610-11
GTACTTT57100.025.37131514-15
AGAGTAC84950.024.58237510-11
AGTACTT57800.023.6744612-13
Produced by FastQC (version 0.11.5)