FastQCFastQC Report
Fri 27 May 2016
SRR940022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898799
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.11715633862520987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.053.165411
GTATCAA19900.052.187491
TCAACGC23650.041.8491444
ATCAACG23750.041.6706163
CAACGCA24150.041.2020075
TATCAAC26000.039.252632
AACGCAG25850.038.26196
GTACATG57900.036.491771
TACATGG56450.036.2640382
ACCGTAT1051.469312E-534.0471658
ACATGGG60400.032.9681783
ACGCAGA31500.030.83167
CATGGGG26000.030.4991664
CGCAGAG32900.029.5196178
CATGGGA38500.027.10114
GTGGTAT7050.027.0913771
GGACCGT1401.0457697E-425.5353746
ACCGTGC2151.214994E-724.9415288
TATAGGG1701.3655521E-524.575092
GCAGAGT39100.024.5216839
Produced by FastQC (version 0.11.5)