##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940022_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 898799 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.467769768324175 34.0 31.0 34.0 28.0 34.0 2 31.418042298667444 34.0 31.0 34.0 28.0 34.0 3 31.634619086136055 34.0 31.0 34.0 28.0 34.0 4 34.732427383653075 37.0 35.0 37.0 32.0 37.0 5 34.89552280320739 37.0 37.0 37.0 32.0 37.0 6 34.85337656138914 37.0 37.0 37.0 32.0 37.0 7 34.8387993311074 37.0 37.0 37.0 32.0 37.0 8 34.76156181749201 37.0 37.0 37.0 32.0 37.0 9 36.33824581469272 39.0 39.0 39.0 32.0 39.0 10-11 36.38228791976849 39.0 39.0 39.0 32.0 39.0 12-13 36.32440957321937 39.0 39.0 39.0 32.0 39.0 14-15 37.833603508682145 41.0 40.0 41.0 32.0 41.0 16-17 37.89059567266986 41.0 40.0 41.0 32.0 41.0 18-19 37.92656589515565 41.0 40.0 41.0 32.0 41.0 20-21 37.970903394418556 41.0 40.0 41.0 32.0 41.0 22-23 38.01910382632825 41.0 39.5 41.0 32.0 41.0 24-25 38.11406610376736 41.0 39.0 41.0 32.0 41.0 26-27 38.17672916859053 41.0 39.0 41.0 32.0 41.0 28-29 38.14605378955695 41.0 39.0 41.0 32.0 41.0 30-31 38.093663878130705 41.0 39.0 41.0 32.0 41.0 32-33 38.049339173719595 41.0 39.0 41.0 32.0 41.0 34-35 37.96660988719391 41.0 39.0 41.0 31.0 41.0 36-37 37.82741525079578 41.0 39.0 41.0 31.0 41.0 38-39 37.72049757509744 41.0 38.0 41.0 31.0 41.0 40-41 37.61915456069711 41.0 38.0 41.0 30.0 41.0 42-43 37.4447585055168 40.5 38.0 41.0 30.0 41.0 44-45 37.28316063992061 40.0 38.0 41.0 30.0 41.0 46-47 37.11455453332725 40.0 37.5 41.0 30.0 41.0 48-49 36.95287656083285 40.0 37.0 41.0 30.0 41.0 50-51 36.773298034376985 40.0 36.5 41.0 30.0 41.0 52-53 36.88142287652745 40.0 36.5 41.0 30.0 41.0 54-55 36.67057540117423 40.0 36.0 41.0 30.0 41.0 56-57 36.535592496208835 40.0 35.5 41.0 30.0 41.0 58-59 36.32206088346783 39.5 35.0 41.0 30.0 41.0 60-61 36.05609485546824 39.0 35.0 41.0 30.0 41.0 62-63 35.745472569506646 39.0 35.0 41.0 30.0 41.0 64-65 35.410616834242134 37.5 35.0 41.0 30.0 41.0 66-67 35.07047404369609 37.0 35.0 40.0 29.0 41.0 68-69 34.70082076192786 36.5 35.0 39.5 29.0 41.0 70-71 34.315405335341936 36.0 35.0 39.0 28.5 41.0 72-73 33.94792384059173 35.5 34.0 39.0 28.0 41.0 74-75 33.58393033370086 35.0 34.0 37.5 28.0 40.0 76-77 33.23467816497349 35.0 34.0 37.0 27.0 39.0 78-79 32.91562351537996 35.0 34.0 36.5 27.0 39.0 80-81 32.62163620564776 35.0 34.0 36.0 27.0 38.0 82-83 32.34266782673323 35.0 34.0 36.0 26.0 37.0 84-85 32.129758155049124 35.0 34.0 35.0 26.0 37.0 86-87 31.938862304030156 35.0 33.5 35.0 26.0 36.5 88-89 31.781451692758893 35.0 33.0 35.0 25.0 36.0 90-91 31.64663567716475 35.0 33.0 35.0 25.0 36.0 92-93 31.506588792377386 35.0 33.0 35.0 25.0 36.0 94-95 31.400867157173074 35.0 33.0 35.0 25.0 35.5 96-97 31.300795839781753 35.0 33.0 35.0 24.0 35.0 98-99 31.232939734022846 35.0 33.0 35.0 24.0 35.0 100-101 31.12937486579313 35.0 33.0 35.0 23.5 35.0 102-103 30.439444191637953 34.0 31.0 35.0 23.0 35.0 104-105 31.348882230621086 35.0 33.5 35.0 25.0 35.0 106-107 31.459441432400347 35.0 34.0 35.0 25.0 35.0 108-109 31.44470621351381 35.0 34.0 35.0 25.0 35.0 110-111 31.39263005410553 35.0 34.0 35.0 24.0 35.0 112-113 31.343344841282644 35.0 34.0 35.0 24.0 35.0 114-115 31.274859562594084 35.0 34.0 35.0 24.0 35.0 116-117 31.193299614263033 35.0 34.0 35.0 23.5 35.0 118-119 31.101410326446736 35.0 33.0 35.0 23.0 35.0 120-121 31.011500902871497 35.0 33.0 35.0 20.5 35.0 122-123 30.87504547735367 35.0 33.0 35.0 18.5 35.0 124-125 29.621442613977095 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20748153566529126 1101 2 0.3624236277931452 1101 3 0.5079062953334557 1101 4 0.360184051907801 1101 5 0.3941604032257473 1101 6 0.4893949304402554 1101 7 0.44854642151107527 1101 8 0.40071484757890374 1101 9 0.48096234596705756 1101 10-11 0.22142412559631452 1101 12-13 0.3664168447703915 1101 14-15 0.3969165633685918 1101 16-17 0.4177644382863761 1101 18-19 0.5109955038554332 1101 20-21 0.18013950109142485 1101 22-23 0.2911005363247199 1101 24-25 0.2750842059794749 1101 26-27 0.3142437639556306 1101 28-29 0.3883212699449672 1101 30-31 0.4842379403324344 1101 32-33 0.4874311229433914 1101 34-35 0.44802630553775913 1101 36-37 0.4859377262236535 1101 38-39 0.5008798783450175 1101 40-41 0.5237297343826981 1101 42-43 0.4322318904543039 1101 44-45 0.5624872449459204 1101 46-47 0.46260968413209014 1101 48-49 0.3702974437836346 1101 50-51 0.3221687903170647 1101 52-53 0.5153922780314772 1101 54-55 0.48927136789735926 1101 56-57 0.3709942301300089 1101 58-59 0.3024018298103428 1101 60-61 0.2810922328640757 1101 62-63 0.2875229168266955 1101 64-65 0.2679731940338925 1101 66-67 0.2598578945817138 1101 68-69 0.22594901187735417 1101 70-71 0.32509486093153583 1101 72-73 0.3599986044488759 1101 74-75 0.3585543314290689 1101 76-77 0.3667393923446767 1101 78-79 0.3248282326107983 1101 80-81 0.33969549783673614 1101 82-83 0.3470075133898405 1101 84-85 0.28630208039954397 1101 86-87 0.28579731492486005 1101 88-89 0.23219076366758173 1101 90-91 0.23176323313526837 1101 92-93 0.2556712698482073 1101 94-95 0.2674791722346157 1101 96-97 0.20177374481069776 1101 98-99 0.2983472795047817 1101 100-101 0.21907039217001056 1101 102-103 0.08433871702059648 1101 104-105 0.2588359592419778 1101 106-107 0.3846349642512834 1101 108-109 0.33816425502212155 1101 110-111 0.2903355275401971 1101 112-113 0.30663898099079745 1101 114-115 0.16038666465739126 1101 116-117 0.2254072465438952 1101 118-119 0.2749730177803009 1101 120-121 0.3030760694764787 1101 122-123 0.22417915682158807 1101 124-125 0.1998896994110524 1102 1 -0.0015328930526834483 1102 2 0.11570646284849317 1102 3 0.2307669629969027 1102 4 0.16193500901722757 1102 5 0.1032888134519041 1102 6 0.31170278778904503 1102 7 0.2033420258477321 1102 8 0.26584203753243685 1102 9 0.34426918418706975 1102 10-11 0.26088812755411084 1102 12-13 0.26598656457702674 1102 14-15 0.30100824808233284 1102 16-17 0.42437536576061774 1102 18-19 0.41033265109942363 1102 20-21 0.18395201492005953 1102 22-23 0.20712130777838667 1102 24-25 0.2806827094693176 1102 26-27 0.32690744475376476 1102 28-29 0.29961222785897235 1102 30-31 0.29333860947093626 1102 32-33 0.3113120927248829 1102 34-35 0.28121688261943234 1102 36-37 0.2754541093892584 1102 38-39 0.31035894932868047 1102 40-41 0.11901534187060747 1102 42-43 0.29679043001594607 1102 44-45 0.2324328355377574 1102 46-47 0.28964693597087887 1102 48-49 0.0659865122865213 1102 50-51 0.07018417111587638 1102 52-53 0.14713562519904144 1102 54-55 0.2781151763547882 1102 56-57 0.17642947187137992 1102 58-59 0.24990245000383737 1102 60-61 0.014690210027545447 1102 62-63 -0.016351364922186917 1102 64-65 0.006383956570175542 1102 66-67 0.028274289145464593 1102 68-69 0.06803433709341533 1102 70-71 0.022939101848216126 1102 72-73 -0.05032141539732038 1102 74-75 -0.1420105475158664 1102 76-77 -0.09627402730171042 1102 78-79 -0.12481624565561589 1102 80-81 -0.12297681538444749 1102 82-83 -0.043203570306104666 1102 84-85 -0.09208384087275334 1102 86-87 -0.053775388515369116 1102 88-89 -0.030063924660048258 1102 90-91 -0.12270249530714494 1102 92-93 -0.027801998135728923 1102 94-95 -0.0060873426801997255 1102 96-97 -0.004406651040373077 1102 98-99 -0.09547978372813404 1102 100-101 -0.14220573138594617 1102 102-103 -0.10366749497159944 1102 104-105 0.004088126566831107 1102 106-107 0.03318555527892997 1102 108-109 0.023657845238485464 1102 110-111 0.04092722566352336 1102 112-113 0.014514432726333126 1102 114-115 0.006281570354456534 1102 116-117 0.1014033275914521 1102 118-119 0.10187617372711699 1102 120-121 0.0977023442255529 1102 122-123 0.09099763121262683 1102 124-125 0.22051322854673572 1103 1 0.10098509255882604 1103 2 0.07973523982690978 1103 3 0.21457991263719123 1103 4 0.15294220326182995 1103 5 0.07810895733679502 1103 6 0.3467747302350901 1103 7 0.2186297956319052 1103 8 0.3638636202662511 1103 9 0.1527224215971401 1103 10-11 0.37509676064763653 1103 12-13 0.46742541349789235 1103 14-15 0.5118895430463652 1103 16-17 0.577702703890111 1103 18-19 0.4557463201641738 1103 20-21 0.35256712283372593 1103 22-23 0.38068245885752106 1103 24-25 0.36746328500889547 1103 26-27 0.4330225526674383 1103 28-29 0.46687841490933124 1103 30-31 0.5388422065932374 1103 32-33 0.5302869128687675 1103 34-35 0.46511976031727187 1103 36-37 0.5151123827705462 1103 38-39 0.5648553522063722 1103 40-41 0.4886196584173774 1103 42-43 0.37862496239004173 1103 44-45 0.36147959812768704 1103 46-47 0.3233699575536093 1103 48-49 0.24224550509227782 1103 50-51 0.25498632938925425 1103 52-53 0.2653910208824968 1103 54-55 0.4692122986569416 1103 56-57 0.34504457978505343 1103 58-59 0.27688086727003025 1103 60-61 0.25704632513546244 1103 62-63 0.38427813148069134 1103 64-65 0.2226609349874451 1103 66-67 0.2063318431022978 1103 68-69 0.16155951694953075 1103 70-71 0.11331679968994024 1103 72-73 0.17629728963865432 1103 74-75 0.18397866111722294 1103 76-77 0.2364597856479307 1103 78-79 0.3518024593803588 1103 80-81 0.3725267817378466 1103 82-83 0.293577005233459 1103 84-85 0.2352542886236435 1103 86-87 0.2546778488947048 1103 88-89 0.3458353559154901 1103 90-91 0.37280110181515624 1103 92-93 0.3247159874757841 1103 94-95 0.4165745278233999 1103 96-97 0.37419047126178384 1103 98-99 0.23410654720711932 1103 100-101 0.3802762830025479 1103 102-103 0.3023576848845124 1103 104-105 0.34221762296970937 1103 106-107 0.38885102290483076 1103 108-109 0.4125966941593475 1103 110-111 0.41457830480021585 1103 112-113 0.3324101161795703 1103 114-115 0.3758858869012194 1103 116-117 0.4431299462964873 1103 118-119 0.43056322408682846 1103 120-121 0.34185702048454303 1103 122-123 0.2811954729392454 1103 124-125 0.18364272494961398 1104 1 -0.30239340225011446 1104 2 -0.15571703137580073 1104 3 -0.053436469808605835 1104 4 -0.06282667009115528 1104 5 0.0415661641974836 1104 6 0.05444084712771513 1104 7 0.005672616465062674 1104 8 -0.03912946858174138 1104 9 0.029946390924678212 1104 10-11 0.09237148663245875 1104 12-13 -0.0011366914802337647 1104 14-15 2.065882980986089E-4 1104 16-17 -0.1174238723510328 1104 18-19 -0.12215491744283469 1104 20-21 -0.11638846547577941 1104 22-23 -0.1415056931016636 1104 24-25 -0.028020541787668662 1104 26-27 -0.05661071932916428 1104 28-29 -0.08710957519473794 1104 30-31 -0.020727631120848855 1104 32-33 0.03125427413799997 1104 34-35 0.09103173390350605 1104 36-37 0.024507903137831022 1104 38-39 -4.3595623532155514E-4 1104 40-41 0.05272513195129136 1104 42-43 -0.0035481271964528105 1104 44-45 -0.027113077774366445 1104 46-47 0.11535651373210953 1104 48-49 0.05851879099733992 1104 50-51 0.20404543112411488 1104 52-53 0.19022647330755404 1104 54-55 0.22624038021223214 1104 56-57 0.24031762567682335 1104 58-59 0.08790963194007162 1104 60-61 -0.09728176072776051 1104 62-63 0.03500186387202575 1104 64-65 0.11150208618966673 1104 66-67 0.012824202679134089 1104 68-69 -0.0949193034159066 1104 70-71 -0.013880629706150671 1104 72-73 -0.04372104983860936 1104 74-75 0.0844804581398293 1104 76-77 -0.07468783175857396 1104 78-79 -0.03821375690238682 1104 80-81 -0.01640975936288669 1104 82-83 -0.019760348835376362 1104 84-85 -0.0657400741410612 1104 86-87 -0.05159526777022094 1104 88-89 0.014643166809634067 1104 90-91 0.013541973617286374 1104 92-93 -0.1625436915218188 1104 94-95 -0.1020734280633917 1104 96-97 -0.16022028071832395 1104 98-99 -0.05411980071369271 1104 100-101 -0.1473275535176306 1104 102-103 -0.2606973458556503 1104 104-105 -0.24352943379035707 1104 106-107 -0.0935499254553882 1104 108-109 -0.13758070028096014 1104 110-111 -0.11306284147865497 1104 112-113 -0.15068548603839815 1104 114-115 -0.24253336401113756 1104 116-117 -0.16924666225414242 1104 118-119 -0.2204193687952376 1104 120-121 -0.23107663430248948 1104 122-123 -0.21578474442663875 1104 124-125 -0.14071254703467773 1105 1 -0.01970964085305127 1105 2 0.07552084131087966 1105 3 0.1203779223130752 1105 4 0.14446953888462133 1105 5 -0.06491425475555701 1105 6 -0.1823312532707888 1105 7 -0.09364616756965916 1105 8 0.06672502452263984 1105 9 -0.08129106447810841 1105 10-11 0.047750797696743064 1105 12-13 0.11571584038982508 1105 14-15 0.1459951505464261 1105 16-17 0.0764540512442835 1105 18-19 0.13077389392397976 1105 20-21 -0.06637699016855692 1105 22-23 -0.00879376504140339 1105 24-25 0.050053275734086355 1105 26-27 0.13192336233328916 1105 28-29 0.15127950031278203 1105 30-31 0.1347001891967068 1105 32-33 0.16904402564544085 1105 34-35 0.21442258286759852 1105 36-37 0.2567133528655532 1105 38-39 0.1088950333534413 1105 40-41 0.1704357038388764 1105 42-43 0.16304986149514633 1105 44-45 -0.014101644195527285 1105 46-47 0.03806771154553701 1105 48-49 0.14664132641431138 1105 50-51 0.03248958810632274 1105 52-53 0.09445721043955757 1105 54-55 0.19175447397591938 1105 56-57 0.12321211544293931 1105 58-59 0.12309448870737327 1105 60-61 0.026369890673272778 1105 62-63 0.0016608803338797884 1105 64-65 0.14239152682060308 1105 66-67 0.12353222913493767 1105 68-69 0.14547273480729928 1105 70-71 0.13299287067983556 1105 72-73 0.07596640452096182 1105 74-75 0.09165513317999086 1105 76-77 0.006596463761887605 1105 78-79 0.03669391903493846 1105 80-81 0.06913865600151325 1105 82-83 -0.06044813702207108 1105 84-85 0.10182549159159038 1105 86-87 0.01836759396276122 1105 88-89 -0.014102477789670331 1105 90-91 0.011202575757955913 1105 92-93 0.016455453046184232 1105 94-95 -0.027726477365483504 1105 96-97 -0.004693355474739036 1105 98-99 -0.08123543008213119 1105 100-101 -0.06047773399291856 1105 102-103 -0.11514350580057098 1105 104-105 0.06659596002132417 1105 106-107 0.024390152576700075 1105 108-109 -0.004691426563585566 1105 110-111 0.0515556567165909 1105 112-113 0.09523034779915562 1105 114-115 0.08422600922844481 1105 116-117 0.09211128387450884 1105 118-119 0.03518527557455897 1105 120-121 0.015426005018174038 1105 122-123 0.09365473897589638 1105 124-125 0.14359340552014288 1106 1 -0.18752382633345732 1106 2 0.06368369749912972 1106 3 -0.011829370894886893 1106 4 0.051612992657709356 1106 5 0.039803300492408766 1106 6 0.11515454692967353 1106 7 0.14681669816467746 1106 8 0.16612660439949423 1106 9 0.001175166250732218 1106 10-11 0.1548623317523976 1106 12-13 0.10915141625732616 1106 14-15 0.13449659439159234 1106 16-17 0.15208737423602514 1106 18-19 0.1015837598015068 1106 20-21 0.12836908400451819 1106 22-23 0.10412596432712462 1106 24-25 0.1467237807219135 1106 26-27 0.33648847855686626 1106 28-29 0.377855797386907 1106 30-31 0.35349493646413066 1106 32-33 0.2856240814900346 1106 34-35 0.35651007272488044 1106 36-37 0.30392619473467875 1106 38-39 0.32297043548221893 1106 40-41 0.13594152694994222 1106 42-43 0.1291269580537886 1106 44-45 0.21413925122712385 1106 46-47 0.0971639360694283 1106 48-49 0.075241094921779 1106 50-51 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2216 26-27 -1.265163170598612 2216 28-29 -1.2088977602670354 2216 30-31 -1.3435118131036248 2216 32-33 -1.2746224606033323 2216 34-35 -1.4112116987872838 2216 36-37 -1.3214935751815347 2216 38-39 -1.3039073075687142 2216 40-41 -1.335043643950442 2216 42-43 -1.2932904179270608 2216 44-45 -1.2421149049106575 2216 46-47 -1.3046448150942922 2216 48-49 -1.3952195713902356 2216 50-51 -1.2868703794184597 2216 52-53 -1.4705845675787756 2216 54-55 -1.2708694223740054 2216 56-57 -1.4080994098775932 2216 58-59 -1.333587457099604 2216 60-61 -1.2896376868782298 2216 62-63 -1.3050946421450718 2216 64-65 -1.2519819720461527 2216 66-67 -1.2169307119022434 2216 68-69 -1.2198227558729897 2216 70-71 -1.384747564352807 2216 72-73 -1.206422553903991 2216 74-75 -1.2376241537182864 2216 76-77 -1.2004761995444966 2216 78-79 -1.3203373045366646 2216 80-81 -1.4104628459774489 2216 82-83 -1.5147155839961286 2216 84-85 -1.3897334881452323 2216 86-87 -1.2753301835143205 2216 88-89 -1.3016536431764933 2216 90-91 -1.3569065883433922 2216 92-93 -1.5292102889787813 2216 94-95 -1.4248569871587904 2216 96-97 -1.530701440451562 2216 98-99 -1.5809411233942647 2216 100-101 -1.557484770290742 2216 102-103 -1.6702989470914602 2216 104-105 -1.5032807356249691 2216 106-107 -1.5702404417874973 2216 108-109 -1.5039395913753317 2216 110-111 -1.4013197534522135 2216 112-113 -1.4933678051726638 2216 114-115 -1.5608807192313492 2216 116-117 -1.6123853502041747 2216 118-119 -1.6018990585142738 2216 120-121 -1.633334185773748 2216 122-123 -1.6781806184206758 2216 124-125 -1.7701036953898424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16622.0 3 2854.0 4 1831.0 5 1368.0 6 1375.0 7 1663.0 8 1782.0 9 1940.0 10 2306.0 11 2728.0 12 3248.0 13 3363.0 14 3455.0 15 3324.0 16 2799.0 17 2634.0 18 2166.0 19 1943.0 20 1908.0 21 1729.0 22 1824.0 23 2739.0 24 5275.0 25 9592.0 26 9926.0 27 6643.0 28 6897.0 29 8419.0 30 9177.0 31 11125.0 32 14322.0 33 20110.0 34 30629.0 35 57471.0 36 145736.0 37 334177.0 38 162950.0 39 749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8545061893312 18.260308130732923 13.32554100988512 23.559644670050762 2 15.538704602495532 19.95544569358545 39.98960845506047 24.51624124885855 3 19.593259874977605 25.240635283826663 27.238723333693173 27.92738150750257 4 12.874884441028724 16.244194205901024 35.293881778995555 35.587039574074694 5 14.27951408209324 39.023223101132 31.7941036381523 14.90315917862246 6 32.93389046078774 35.247512850365865 17.13434058534001 14.684256103506385 7 30.12844485997952 32.93397444459285 19.019745336360803 17.917835359066828 8 26.80270457195616 36.43242412463929 18.39318310656018 18.371688196844367 9 26.916195814915156 18.269307892485383 18.536668508484244 36.27782778411521 10-11 25.537190358624095 28.040090028918783 25.20737373355091 21.215345878906206 12-13 25.7225787352062 24.9988870562415 26.067034828461978 23.211499380090324 14-15 22.969425025801375 26.047563543703774 23.941170088767848 27.041841341727007 16-17 23.380701468343553 29.43665177381488 24.408436228304133 22.774210529537427 18-19 23.432580201499363 27.9996281929901 25.964931029243065 22.60286057626747 20-21 24.477455378666143 26.959629418449822 25.757836706234084 22.80507849664995 22-23 24.19309476802954 26.80298174142833 25.696997374129467 23.306926116412665 24-25 23.61233200595234 27.012212536095653 25.828815171892405 23.546640286059606 26-27 23.27002104227706 27.35273466144857 25.758873976678792 23.618370319595577 28-29 23.301294408181136 27.443267069114285 25.875701877217317 23.379736645487263 30-31 23.492588745619535 26.98505509691683 25.780194262042905 23.742161895420725 32-33 23.270158237231787 27.171985840262902 25.77320275873426 23.784653163771054 34-35 23.85451102177428 27.238431059249756 25.603329620649788 23.303728298326178 36-37 23.90510745868771 26.96097084440409 25.48321917655803 23.65070252035017 38-39 23.523615969076175 26.964937530911865 25.637278164776863 23.8741683352351 40-41 23.699023530511333 26.79661039579504 25.93090610493709 23.573459968756534 42-43 23.451977331978803 26.84823040999832 25.721060496644636 23.978731761378242 44-45 23.439449835793052 27.05951340443868 25.859186492692054 23.641850267076215 46-47 23.711750328130538 27.163588999740167 25.49024844447486 23.634412227654437 48-49 23.655811075773997 27.008354791541944 25.67879899315192 23.65703513953214 50-51 23.652088282584387 27.051980349502898 25.722734378911877 23.57319698900084 52-53 23.735359850982185 26.940544762928482 25.747356274643664 23.576739111445676 54-55 23.542846524090653 26.973940068667495 25.771952928661364 23.711260478580492 56-57 23.62937138418774 27.216168126437562 25.591855402005436 23.562605087369263 58-59 23.6629154968603 26.815856620964933 25.726504000988143 23.79472388118662 60-61 23.490041915308154 26.96972461853954 25.980926142489796 23.55930732366251 62-63 23.36597370557342 27.113004607561606 26.000034496898895 23.520987189966075 64-65 23.2295993745357 27.050830103529922 25.903960068222354 23.815610453712026 66-67 23.28773068880285 27.318573404751383 25.64283480550526 23.750861100940508 68-69 23.490111034873063 26.829615754675977 26.052265911631757 23.628007298819202 70-71 23.312175771540623 26.84476707855934 26.025303382965593 23.817753766934445 72-73 23.42574370906728 27.061901987916382 25.792838070693463 23.719516232322878 74-75 23.302390920344383 27.375272959928786 25.710780700168296 23.611555419558535 76-77 23.542914876819772 27.111333159379036 25.692511490163326 23.653240473637858 78-79 23.514378525634964 27.099912419026552 25.838612859824817 23.547096195513674 80-81 23.565587908651654 27.336233316196058 25.68960835764823 23.408570417504055 82-83 23.622729402285984 27.28473069984988 25.725762207505614 23.36677769035852 84-85 23.55837682920519 27.250142853407088 25.637832812273096 23.553647505114625 86-87 23.506158990256125 27.230599472825823 25.77182828834213 23.491413248575924 88-89 23.447328356415017 27.34266839389771 25.70090857694859 23.509094672738684 90-91 23.462330541271893 27.36107069147897 25.747988864399073 23.428609902850063 92-93 23.476421575256133 27.063226939520966 25.863117646436873 23.59723383878602 94-95 23.31326440900542 27.03417652492293 26.102418286833522 23.550140779238124 96-97 23.450968695798302 27.053369665801757 25.93067635928189 23.56498527911805 98-99 23.2908965044429 27.085225350357035 25.748737913350073 23.875140231849993 100-101 23.026662081843543 27.202189586887403 26.048861750890534 23.722286580378515 102-103 23.163455749213455 26.87109996227251 26.15344293967274 23.8120013488413 104-105 22.979247870952303 27.145754563437414 26.00462394938695 23.87037361622333 106-107 23.072916492769785 27.182614541006835 25.928553701026463 23.815915265196917 108-109 23.260018128027944 27.10156870316717 25.72304846882708 23.915364699977797 110-111 23.03182355880353 27.363174944442033 25.897357143413558 23.70764435334088 112-113 23.048133519853366 27.518326483507554 25.957167165796946 23.47637283084213 114-115 22.870341624410102 27.684418317148427 25.799091330923122 23.64614872751834 116-117 22.833707301305058 27.575469110505168 26.0274475614933 23.563376026696474 118-119 22.814485882496967 27.668803490145017 25.791459383659976 23.72525124369804 120-121 22.78833323874038 27.800758301644695 25.95085395181546 23.46005450779947 122-123 22.809146493115424 27.840919523346123 25.81363271923836 23.53630126430009 124-125 22.866116760095146 27.911977855364373 25.68811116829645 23.533794216244036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 764.0 2 757.0 3 673.5 4 520.5 5 422.0 6 363.0 7 329.5 8 330.5 9 330.0 10 283.5 11 270.0 12 286.0 13 275.5 14 285.5 15 320.5 16 366.0 17 409.5 18 458.5 19 546.0 20 690.0 21 917.0 22 1214.5 23 1649.5 24 2136.5 25 2753.0 26 3590.5 27 4511.5 28 5565.0 29 6801.0 30 8337.0 31 10084.5 32 12258.5 33 13693.0 34 14470.0 35 15538.0 36 17312.5 37 19279.5 38 20240.0 39 20985.0 40 22040.5 41 23473.5 42 24352.0 43 25509.5 44 27452.0 45 29507.0 46 30913.0 47 31842.5 48 32722.5 49 33248.5 50 33516.5 51 33328.0 52 33908.5 53 35253.5 54 36324.0 55 35691.0 56 32826.5 57 29880.5 58 28298.0 59 25640.5 60 21644.0 61 18277.0 62 15147.5 63 11778.0 64 8971.5 65 7018.5 66 5207.5 67 4026.0 68 2990.0 69 2022.0 70 1486.5 71 1007.5 72 766.0 73 639.5 74 481.5 75 384.5 76 271.5 77 151.5 78 98.0 79 61.0 80 42.5 81 31.5 82 25.5 83 26.0 84 17.0 85 14.0 86 14.0 87 11.0 88 9.0 89 5.0 90 6.0 91 8.0 92 6.0 93 2.5 94 1.5 95 2.5 96 2.0 97 1.0 98 4.0 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05329333922267381 2 0.21317335689069525 3 0.026702299401757237 4 0.11003572545140793 5 0.023475771557378233 6 0.04238990030028961 7 0.0399421895217952 8 0.10146873772667749 9 0.12627962425414357 10-11 0.04684028353391581 12-13 0.0312639422162241 14-15 0.011849145359529772 16-17 0.029984457036556562 18-19 0.053794007336456756 20-21 0.19164462799802848 22-23 0.11142647021191612 24-25 0.07393199146861534 26-27 0.01468626467096648 28-29 0.005396089670771774 30-31 0.007176242964222256 32-33 0.00595238757497505 34-35 0.008678247305571101 36-37 0.0038940853294229297 38-39 0.008288838772628808 40-41 0.005674238622873412 42-43 0.009790843113977652 44-45 0.009512694161876015 46-47 0.016410788173996632 48-49 0.017022715868620236 50-51 0.010458400599021584 52-53 0.012739222006255015 54-55 0.013406779491298943 56-57 0.015798860479373032 58-59 0.016243898802735652 60-61 0.009123285628933721 62-63 0.018635979790809734 64-65 0.029650678294034595 66-67 0.026702299401757237 68-69 0.033099725300094904 70-71 0.024866516317886427 72-73 0.016077009431474668 74-75 0.009623953742716669 76-77 0.010625289970282566 78-79 0.022474435329812338 80-81 0.019247907485433337 82-83 0.021361839521405787 84-85 0.0167445669165186 86-87 0.026090371707133634 88-89 0.027926154791004444 90-91 0.026646669611336906 92-93 0.033099725300094904 94-95 0.02547844401251003 96-97 0.02731422709638084 98-99 0.031931499701268025 100-101 0.020249243712999236 102-103 0.027814895210163785 104-105 0.024087699252001837 106-107 0.03093016347370213 108-109 0.021194950150144803 110-111 0.020916801198043167 112-113 0.026257261078394614 114-115 0.013573668862559927 116-117 0.011515366617007807 118-119 0.02992882724613623 120-121 0.02358703113821889 122-123 0.017745903144084495 124-125 0.017968422305765806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 898799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.59792033033948 #Duplication Level Percentage of deduplicated Percentage of total 1 79.45483633691171 49.737075148781614 2 11.931780215527002 14.938092546613602 3 3.761261249479972 7.063413961096211 4 1.6374610877457623 4.1000663485896105 5 0.8632720690295226 2.701951810025869 6 0.5392047355467925 2.0251857046499935 7 0.35650019367902064 1.562131950516895 8 0.2451099859172556 1.2274700296495198 9 0.18950887954207787 1.0676575569120204 >10 0.9510052815350778 10.499557194578902 >50 0.04694668648559954 1.9604524661128746 >100 0.02151312863826167 2.467980510394013 >500 0.0014223555216714722 0.5317704820773128 >1k 1.7779444020893403E-4 0.11719429000155095 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1053 0.11715633862520987 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.562979042032757E-5 0.0 18-19 0.0 0.0 0.0 2.2251916168131028E-4 0.0 20-21 0.0 0.0 0.0 2.2251916168131028E-4 0.0 22-23 0.0 0.0 0.0 2.2251916168131028E-4 0.0 24-25 0.0 0.0 0.0 2.2251916168131028E-4 0.0 26-27 0.0 0.0 0.0 2.7814895210163785E-4 0.0 28-29 0.0 0.0 0.0 0.0010013362275658963 0.0 30-31 0.0 0.0 0.0 0.0035046767964806366 0.0 32-33 0.0 0.0 0.0 0.00895639625767274 0.0 34-35 0.0 0.0 0.0 0.015353822156010409 0.0 36-37 0.0 0.0 0.0 0.027369856886801165 0.0 38-39 0.0 0.0 0.0 0.04789724955190204 0.0 40-41 0.0 0.0 0.0 0.06948160823498914 0.0 42-43 0.0 0.0 0.0 0.09023152006177132 0.0 44-45 0.0 0.0 0.0 0.11476425763713577 0.0 46-47 0.0 0.0 0.0 0.14758583398512903 0.0 48-49 0.0 0.0 0.0 0.18224319341699313 0.0 50-51 0.0 0.0 0.0 0.22485561287896405 0.0 52-53 0.0 0.0 0.0 0.26418587470613564 0.0 54-55 0.0 0.0 0.0 0.3064645154255846 0.0 56-57 0.0 0.0 0.0 0.353916726654124 0.0 58-59 0.0 0.0 0.0 0.4105478533020175 0.0 60-61 0.0 0.0 0.0 0.48125331692625384 0.0 62-63 0.0 0.0 0.0 0.5519031507600698 0.0 64-65 0.0 0.0 0.0 0.6218297973184216 0.0 66-67 0.0 0.0 0.0 0.7048850744159707 0.0 68-69 0.0 0.0 0.0 0.8008464628910357 0.0 70-71 0.0 0.0 0.0 0.9071549923842817 0.0 72-73 0.0 0.0 0.0 1.011794628164918 0.0 74-75 0.0 0.0 0.0 1.121329685502543 0.0 76-77 0.0 0.0 0.0 1.2458847862536562 0.0 78-79 0.0 0.0 0.0 1.3885751986817965 0.0 80-81 0.0 0.0 0.0 1.5534618974876473 0.0 82-83 0.0 0.0 0.0 1.7299195927009263 0.0 84-85 0.0 0.0 0.0 1.8963639256385465 0.0 86-87 0.0 0.0 0.0 2.0762150380674655 0.0 88-89 0.0 0.0 0.0 2.2808214072334305 0.0 90-91 0.0 0.0 0.0 2.515579122807213 0.0 92-93 0.0 0.0 0.0 2.7770391377827526 0.0 94-95 0.0 0.0 0.0 3.038610412339133 0.0 96-97 0.0 0.0 0.0 3.2925604056079276 0.0 98-99 0.0 0.0 0.0 3.575771668637816 0.0 100-101 0.0 0.0 0.0 3.9155584285251765 0.0 102-103 0.0 0.0 0.0 4.280489853682525 0.0 104-105 0.0 0.0 0.0 4.642472899947597 0.0 106-107 0.0 0.0 0.0 4.995888958487938 0.0 108-109 0.0 0.0 0.0 5.387300163885362 0.0 110-111 0.0 0.0 0.0 5.827721214643097 0.0 112-113 0.0 0.0 0.0 6.312089799832888 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1190 0.0 53.16541 1 GTATCAA 1990 0.0 52.18749 1 TCAACGC 2365 0.0 41.849144 4 ATCAACG 2375 0.0 41.670616 3 CAACGCA 2415 0.0 41.202007 5 TATCAAC 2600 0.0 39.25263 2 AACGCAG 2585 0.0 38.2619 6 GTACATG 5790 0.0 36.49177 1 TACATGG 5645 0.0 36.264038 2 ACCGTAT 105 1.469312E-5 34.047165 8 ACATGGG 6040 0.0 32.968178 3 ACGCAGA 3150 0.0 30.8316 7 CATGGGG 2600 0.0 30.499166 4 CGCAGAG 3290 0.0 29.519617 8 CATGGGA 3850 0.0 27.1011 4 GTGGTAT 705 0.0 27.091377 1 GGACCGT 140 1.0457697E-4 25.535374 6 ACCGTGC 215 1.214994E-7 24.941528 8 TATAGGG 170 1.3655521E-5 24.57509 2 GCAGAGT 3910 0.0 24.521683 9 >>END_MODULE