FastQCFastQC Report
Fri 27 May 2016
SRR940022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898799
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60910.6776821069004304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.4821990233633994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.3239878994079878No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.14307982096108252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.10536282305610041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.074.8304141
GTATCAA45850.059.1512831
TGGTATC8350.046.3897672
TCAACGC60250.044.7071154
TATCAAC60300.044.6700482
ATCAACG60500.044.522383
CAACGCA61700.043.699485
GTGGTAT9250.043.1742861
AACGCAG64350.041.9760256
ACGCAGA75250.035.893787
GTACATG57400.034.8914871
CGCAGAG77750.034.739648
TATAACG1407.95244E-834.0531462
TACATGG57650.034.0088462
GCAGAGT83850.031.999519
ACATGGG59450.031.676013
TAACGCA1551.9432264E-730.7576834
CATGGGA37600.029.9549964
GAGTACT53750.027.1733312-13
AGTACTT53550.027.0526212-13
Produced by FastQC (version 0.11.5)