FastQCFastQC Report
Fri 27 May 2016
SRR940021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396740
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.050.7266541
GGTATCA16800.050.103651
ATCAACG33750.039.0433923
AACGCAG33750.038.8388376
TCAACGC34100.038.8230824
CAACGCA35150.037.630935
TATCAAC37450.036.8124582
GTACATG69200.036.5779461
TACATGG71500.035.2241942
ACATGGG73550.033.075513
ACGCAGA40150.032.351047
CATGGGA33700.029.5513954
CGCAGAG42250.029.473858
CATGGGG41450.027.3350874
ATGGGAG18700.024.8525125
GCAGAGT47250.024.7085329
ATGGGGA20900.023.9469555
CTATAGA3302.7284841E-1123.517381
GCTTATA8150.022.7072011
GTATAGG3557.4578566E-1121.8612271
Produced by FastQC (version 0.11.5)