FastQCFastQC Report
Fri 27 May 2016
SRR940021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396740
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.3356387015478901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.2404169709466329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.1496341480876899No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG15640.11197502756418516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.068.1504441
GTATCAA51300.058.0042571
ATCAACG66350.044.3867843
TCAACGC68200.043.1827434
TATCAAC69650.042.5435832
CAACGCA70100.042.038545
AACGCAG71300.041.3907936
GTGGTAT11050.037.2360421
ACGCAGA81750.036.168767
CGCAGAG83900.035.029188
GTACATG69550.033.4382971
GCAGAGT90800.032.3672689
TACATGG74850.030.905712
GTATAGG4250.030.8681411
ACATGGG74600.029.8882453
TGGTATC13850.029.7028522
GAGTACT52150.027.60743512-13
AGTACTT52150.027.26519412-13
AGAGTAC86000.026.5296710-11
CATGGGG41550.026.1136744
Produced by FastQC (version 0.11.5)