FastQCFastQC Report
Fri 27 May 2016
SRR940020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167546
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18050.048.2753141
GGTATCA11350.046.2739141
TCAACGC20950.040.98784
ATCAACG21050.040.7895773
CAACGCA21400.040.376815
AACGCAG23000.037.049816
TATCAAC23650.036.3380932
GTACATG50450.035.845191
TACATGG52550.033.162012
ACATGGG56100.030.3977893
ACGCAGA28000.029.3696547
CGCAGAG29900.026.7061548
CATGGGA23700.026.6706564
CATGGGG35800.024.3190164
ATGGGAT10050.023.7176515
GTCCTAC2302.2947825E-723.354151
GTGTACG2052.203602E-623.2908631
GCAGAGT35850.021.7629419
GTGTAGC8000.021.6350271
ATTCGCG2750.021.62706680-81
Produced by FastQC (version 0.11.5)