##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940020_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167546 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.719894548052068 34.0 31.0 34.0 30.0 34.0 2 31.731338208515982 34.0 31.0 34.0 30.0 34.0 3 31.919460132620042 34.0 31.0 34.0 30.0 34.0 4 35.12952980011066 37.0 37.0 37.0 33.0 37.0 5 35.15012085176944 37.0 37.0 37.0 33.0 37.0 6 35.15345262627768 37.0 37.0 37.0 33.0 37.0 7 35.06331056763502 37.0 37.0 37.0 33.0 37.0 8 35.05142581106012 37.0 37.0 37.0 33.0 37.0 9 36.71650453172723 39.0 39.0 39.0 34.0 39.0 10-11 36.753804561019436 39.0 39.0 39.0 34.0 39.0 12-13 36.73468026099186 39.0 39.0 39.0 34.0 39.0 14-15 38.249333645098346 41.0 40.0 41.0 34.0 41.0 16-17 38.280941821564205 41.0 40.0 41.0 34.0 41.0 18-19 38.290989391424404 41.0 40.0 41.0 34.0 41.0 20-21 38.303238159353036 41.0 40.0 41.0 34.0 41.0 22-23 38.310064014608415 41.0 40.0 41.0 34.0 41.0 24-25 38.36157761664208 41.0 39.5 41.0 34.0 41.0 26-27 38.39857830012693 41.0 39.5 41.0 34.0 41.0 28-29 38.35372610586649 41.0 39.0 41.0 33.5 41.0 30-31 38.29787477324234 41.0 39.0 41.0 33.0 41.0 32-33 38.25234209187475 41.0 39.0 41.0 33.0 41.0 34-35 38.1694913947716 41.0 39.0 41.0 33.0 41.0 36-37 38.03384577566965 41.0 39.0 41.0 33.0 41.0 38-39 37.931129908371915 41.0 39.0 41.0 33.0 41.0 40-41 37.833001440628465 41.0 38.0 41.0 32.0 41.0 42-43 37.6598459503951 40.5 38.0 41.0 32.0 41.0 44-45 37.49021623130909 40.0 38.0 41.0 31.0 41.0 46-47 37.311805701873844 40.0 38.0 41.0 31.0 41.0 48-49 37.146412646696575 40.0 37.0 41.0 30.5 41.0 50-51 36.96007266523118 40.0 37.0 41.0 30.0 41.0 52-53 37.08135439631501 40.0 37.0 41.0 31.0 41.0 54-55 36.870731431566725 40.0 36.5 41.0 30.0 41.0 56-57 36.735665232890184 40.0 36.0 41.0 30.0 41.0 58-59 36.515708160535 39.5 35.5 41.0 30.5 41.0 60-61 36.25181663077943 39.0 35.0 41.0 30.0 41.0 62-63 35.94253202871664 39.0 35.0 41.0 30.0 41.0 64-65 35.60214929433187 38.0 35.0 41.0 30.0 41.0 66-67 35.261872765612665 37.0 35.0 40.0 30.0 41.0 68-69 34.896523563097304 37.0 35.0 39.5 29.0 41.0 70-71 34.52417806236328 36.0 35.0 39.0 29.0 41.0 72-73 34.15876976153402 36.0 34.5 39.0 29.0 41.0 74-75 33.797048253345054 35.0 34.0 37.5 28.5 40.0 76-77 33.44161857434311 35.0 34.0 37.0 28.0 39.0 78-79 33.12491370789673 35.0 34.0 37.0 28.0 39.0 80-81 32.82799392914711 35.0 34.0 36.0 27.0 38.5 82-83 32.55140097263833 35.0 34.0 36.0 27.0 37.0 84-85 32.33208498851437 35.0 34.0 35.5 27.0 37.0 86-87 32.13960649087916 35.0 34.0 35.0 26.0 36.5 88-89 31.986673758464335 35.0 34.0 35.0 26.0 36.0 90-91 31.848051811234846 35.0 33.0 35.0 26.0 36.0 92-93 31.710023844884912 35.0 33.0 35.0 25.0 36.0 94-95 31.60553631291615 35.0 33.0 35.0 25.0 35.5 96-97 31.50788362942445 35.0 33.0 35.0 25.0 35.0 98-99 31.44276456773438 35.0 33.0 35.0 25.0 35.0 100-101 31.342358673662538 35.0 33.0 35.0 24.0 35.0 102-103 30.63949643097574 34.0 31.5 35.0 24.0 35.0 104-105 31.573843771465963 35.0 33.5 35.0 25.0 35.0 106-107 31.68922166664097 35.0 34.0 35.0 25.0 35.0 108-109 31.67247671612082 35.0 34.0 35.0 25.0 35.0 110-111 31.62446190556946 35.0 34.0 35.0 25.0 35.0 112-113 31.57076423541342 35.0 34.0 35.0 25.0 35.0 114-115 31.50949555734849 35.0 34.0 35.0 25.0 35.0 116-117 31.43332725220248 35.0 34.0 35.0 24.5 35.0 118-119 31.34481853391644 35.0 34.0 35.0 24.0 35.0 120-121 31.248282294659056 35.0 34.0 35.0 24.0 35.0 122-123 31.11029287068775 35.0 33.5 35.0 23.0 35.0 124-125 29.834000972980938 34.5 31.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.06925774639709559 1101 2 0.07569115169974694 1101 3 0.2050217631706026 1101 4 0.10711902626770353 1101 5 0.09499904596705733 1101 6 0.29871488973429194 1101 7 0.24240982988715842 1101 8 0.08825216461124796 1101 9 0.29459602140368446 1101 10-11 0.05614166653072061 1101 12-13 0.0630909477594912 1101 14-15 0.23153137484952424 1101 16-17 0.11773062327101513 1101 18-19 0.21521790001962415 1101 20-21 0.17098740057683415 1101 22-23 0.18462711666596476 1101 24-25 0.10850722178053473 1101 26-27 0.19757694707937645 1101 28-29 0.16309673291944193 1101 30-31 0.26522600417726494 1101 32-33 0.2677242768008341 1101 34-35 0.2826436035515485 1101 36-37 0.23869329609789958 1101 38-39 0.24848530125675694 1101 40-41 0.2670974259558818 1101 42-43 0.29819719255844745 1101 44-45 0.33122053997573886 1101 46-47 0.3118183749048171 1101 48-49 0.2829346497550489 1101 50-51 0.3020979003720612 1101 52-53 0.3260610968341524 1101 54-55 0.29919630282476106 1101 56-57 0.2132136258577475 1101 58-59 0.1426896537456912 1101 60-61 0.1346177600224152 1101 62-63 0.1326332780573054 1101 64-65 0.23024039622743686 1101 66-67 0.15064762768297157 1101 68-69 0.14042427653062362 1101 70-71 0.18422322324295237 1101 72-73 0.16082440334389503 1101 74-75 0.11501311184122898 1101 76-77 0.1767678403868942 1101 78-79 0.14178827751694456 1101 80-81 0.12419228035281549 1101 82-83 0.21513877832607164 1101 84-85 0.18129765060049152 1101 86-87 0.15318384375351712 1101 88-89 0.15137336158507253 1101 90-91 0.18388112129925105 1101 92-93 0.21934664494796507 1101 94-95 0.09256981793956598 1101 96-97 0.0859575204249019 1101 98-99 0.07689995615350398 1101 100-101 0.13572851664620345 1101 102-103 0.04610960668926012 1101 104-105 0.09852309135147763 1101 106-107 0.030594738279685174 1101 108-109 0.112495739591985 1101 110-111 0.028207413905523282 1101 112-113 0.013023661571331502 1101 114-115 0.03984259783983646 1101 116-117 0.11405456738859954 1101 118-119 0.10243204156161667 1101 120-121 -0.0017926999983721714 1101 122-123 0.13077299908357887 1101 124-125 0.008758856595250109 1102 1 0.30342308395523787 1102 2 0.47323606637635507 1102 3 0.48439585235258065 1102 4 0.5025896761818203 1102 5 0.557976937270638 1102 6 0.5242973617217359 1102 7 0.6437810120564649 1102 8 0.6218696082082573 1102 9 0.7638855904756596 1102 10-11 0.7644391533621757 1102 12-13 0.7519797521483369 1102 14-15 0.757672862141078 1102 16-17 0.7343350556573043 1102 18-19 0.7722865608450178 1102 20-21 0.7167759396461193 1102 22-23 0.7600851517801672 1102 24-25 0.8322219523875418 1102 26-27 0.7693189872547137 1102 28-29 0.7356993267960874 1102 30-31 0.7879504682383924 1102 32-33 0.7782455617802952 1102 34-35 0.7329439536669113 1102 36-37 0.7686215999254031 1102 38-39 0.7588737363276223 1102 40-41 0.6203614935491544 1102 42-43 0.5942136440827284 1102 44-45 0.48154033038183286 1102 46-47 0.6121086008589884 1102 48-49 0.5778671505939741 1102 50-51 0.43183284698018554 1102 52-53 0.5493416292604607 1102 54-55 0.6130241483557199 1102 56-57 0.6204589604256299 1102 58-59 0.5748986506552001 1102 60-61 0.4644869349460592 1102 62-63 0.5995298851799689 1102 64-65 0.4299454171459516 1102 66-67 0.5026266873052521 1102 68-69 0.43319339146979985 1102 70-71 0.5304493352133264 1102 72-73 0.6087132543133933 1102 74-75 0.5476593964642049 1102 76-77 0.5323823294399759 1102 78-79 0.42758930780482984 1102 80-81 0.469888893939725 1102 82-83 0.4357782355569526 1102 84-85 0.4834294757348445 1102 86-87 0.4959613241967773 1102 88-89 0.49102970097357 1102 90-91 0.49354356370322705 1102 92-93 0.5432316325755799 1102 94-95 0.4328656906089847 1102 96-97 0.4228211598564684 1102 98-99 0.3982068475226832 1102 100-101 0.4622897526114045 1102 102-103 0.36403773750292956 1102 104-105 0.5146574869770788 1102 106-107 0.6043860892990907 1102 108-109 0.5986311048555564 1102 110-111 0.6254896707664237 1102 112-113 0.608610002604415 1102 114-115 0.6275879423892832 1102 116-117 0.6346386982174863 1102 118-119 0.6763550542077859 1102 120-121 0.5716027155152652 1102 122-123 0.6167111095633686 1102 124-125 0.5310971290616173 1103 1 0.3275307714738176 1103 2 0.39578854980319633 1103 3 0.3683862725596718 1103 4 0.4069769402873291 1103 5 0.453971413115724 1103 6 0.4980627442736534 1103 7 0.40069493025598035 1103 8 0.406329851462786 1103 9 0.5413312923415958 1103 10-11 0.5023733278821041 1103 12-13 0.5849052416656164 1103 14-15 0.7687644457616329 1103 16-17 0.7375901285782902 1103 18-19 0.6430226386671052 1103 20-21 0.5359875936028544 1103 22-23 0.5571718021889254 1103 24-25 0.5951448780492186 1103 26-27 0.6417413468673274 1103 28-29 0.6526036503127841 1103 30-31 0.6424395653377957 1103 32-33 0.6780460370474657 1103 34-35 0.7466480177270043 1103 36-37 0.7140987213530465 1103 38-39 0.7196269701126212 1103 40-41 0.7167112797626842 1103 42-43 0.8221590186034007 1103 44-45 0.758053086610353 1103 46-47 0.7162681424732469 1103 48-49 0.7602319653751834 1103 50-51 0.7246961153051217 1103 52-53 0.7292500749544146 1103 54-55 0.8544504710097343 1103 56-57 0.7789378076863755 1103 58-59 0.6863333070031672 1103 60-61 0.7169887880413199 1103 62-63 0.6307470372342792 1103 64-65 0.5970571523922601 1103 66-67 0.5765755414107048 1103 68-69 0.6411292922881415 1103 70-71 0.6951237790637208 1103 72-73 0.6570931162746589 1103 74-75 0.600643031062198 1103 76-77 0.5604421235777224 1103 78-79 0.5551301887381612 1103 80-81 0.6256703417689877 1103 82-83 0.6073476362429204 1103 84-85 0.6804438635712771 1103 86-87 0.6682384607004508 1103 88-89 0.6327964906215513 1103 90-91 0.653200342076758 1103 92-93 0.5455514333114131 1103 94-95 0.5054623551771087 1103 96-97 0.5488386173399711 1103 98-99 0.5356232255811051 1103 100-101 0.5080394523704221 1103 102-103 0.5572142522134591 1103 104-105 0.6541056391630775 1103 106-107 0.6715239749418274 1103 108-109 0.6002524759645809 1103 110-111 0.6079158598766021 1103 112-113 0.6978714958665009 1103 114-115 0.7345486474635265 1103 116-117 0.6736767731016045 1103 118-119 0.7023267717630297 1103 120-121 0.6934112155108458 1103 122-123 0.6520578838282134 1103 124-125 0.4727114833027031 1104 1 -0.051802635252663265 1104 2 -0.008939803804995705 1104 3 0.0831639830603983 1104 4 -0.04352947243904737 1104 5 0.0462876091488198 1104 6 0.08209101898420812 1104 7 0.27209270277391084 1104 8 0.06833238352641757 1104 9 0.2623763190383528 1104 10-11 0.056476692957971864 1104 12-13 0.05356682585672701 1104 14-15 0.0742315472827002 1104 16-17 0.12280943648089959 1104 18-19 0.07795640135036308 1104 20-21 0.10886928384707062 1104 22-23 0.19212093156269106 1104 24-25 0.15936938769124254 1104 26-27 0.15723601668990028 1104 28-29 0.07769263950564209 1104 30-31 0.10804753583374804 1104 32-33 0.273082825363268 1104 34-35 0.2532339060593287 1104 36-37 0.17320382951619706 1104 38-39 0.11903202690057668 1104 40-41 0.23683774533212443 1104 42-43 0.17674425557152773 1104 44-45 0.22390852962974606 1104 46-47 0.24193326788379466 1104 48-49 0.19251921698524654 1104 50-51 0.09117838168555181 1104 52-53 0.05694819058531664 1104 54-55 0.15993729693953895 1104 56-57 -0.013555219964494825 1104 58-59 0.018287078493600006 1104 60-61 -0.08743681882828014 1104 62-63 -0.06771721112813367 1104 64-65 -0.06246446189004473 1104 66-67 -0.15589358759610406 1104 68-69 -0.08222257281525458 1104 70-71 -0.10985594607893745 1104 72-73 0.05340602796434979 1104 74-75 -0.09469375174912642 1104 76-77 -0.06474732248748438 1104 78-79 -0.03673395136935653 1104 80-81 -0.06544954593950081 1104 82-83 0.006442148270217274 1104 84-85 -0.03967640945123918 1104 86-87 -0.026574937218853734 1104 88-89 0.02598491835682637 1104 90-91 -0.024691806691151896 1104 92-93 -0.00516323247453343 1104 94-95 -0.017538140226644572 1104 96-97 0.030885735741467357 1104 98-99 0.04980920382003262 1104 100-101 -0.057248111160522086 1104 102-103 0.012567135646786909 1104 104-105 -0.056889342580372926 1104 106-107 0.07066389897991598 1104 108-109 0.02554490179163338 1104 110-111 0.09519957944137758 1104 112-113 0.049470319741288904 1104 114-115 0.007878265268708162 1104 116-117 -0.10674331093354539 1104 118-119 -0.10210135799605524 1104 120-121 -0.1152987164170085 1104 122-123 -0.10822220277569983 1104 124-125 -0.048296846819681605 1105 1 0.13592953031250588 1105 2 0.2876822655804965 1105 3 0.30678979575181486 1105 4 0.15430424597121117 1105 5 0.06531354363201558 1105 6 0.09656946363163854 1105 7 0.1083606804931847 1105 8 0.20378274442408895 1105 9 0.10757942472853443 1105 10-11 0.05213450285534549 1105 12-13 0.11622108050899982 1105 14-15 0.19712638546083383 1105 16-17 0.1647400599473059 1105 18-19 0.146361735113544 1105 20-21 -0.052847353267182484 1105 22-23 0.005632430138810207 1105 24-25 0.011161338687969646 1105 26-27 0.17260909354408938 1105 28-29 0.28587462865185387 1105 30-31 0.3319778279549013 1105 32-33 0.41217081991845816 1105 34-35 0.36809989033386614 1105 36-37 0.4514006396464083 1105 38-39 0.45216520978525665 1105 40-41 0.419384581432638 1105 42-43 0.45429964396190314 1105 44-45 0.3723935205849216 1105 46-47 0.32054134912606713 1105 48-49 0.25954700521084817 1105 50-51 0.2235293210941478 1105 52-53 0.42448396691831647 1105 54-55 0.44877387208327235 1105 56-57 0.28589335883299327 1105 58-59 0.26765461203110874 1105 60-61 0.24067558781295162 1105 62-63 0.31117161805890703 1105 64-65 0.27221462574629385 1105 66-67 0.25938769766530356 1105 68-69 0.25485764855920934 1105 70-71 0.2261926273453838 1105 72-73 0.27297420449077947 1105 74-75 0.2663588515500024 1105 76-77 0.17015587096441465 1105 78-79 0.13895851545134263 1105 80-81 0.0893769007855596 1105 82-83 0.04280684298481674 1105 84-85 0.04960904896940832 1105 86-87 0.08112704906166357 1105 88-89 0.02871426300962554 1105 90-91 -0.0041501915173327575 1105 92-93 0.03323699678056968 1105 94-95 -0.004711496485487743 1105 96-97 0.06478772918620379 1105 98-99 -0.021715700806261395 1105 100-101 7.478320988383302E-4 1105 102-103 0.008762262184855985 1105 104-105 0.03308768713323573 1105 106-107 -0.008237915359273984 1105 108-109 -0.030715956482147533 1105 110-111 -0.03281342362132733 1105 112-113 -0.03173322475748108 1105 114-115 -0.13864059454538236 1105 116-117 -0.12867881502610956 1105 118-119 -0.12726053805085868 1105 120-121 -0.1011135571026287 1105 122-123 -0.21036027524180412 1105 124-125 -0.14314801011020606 1106 1 0.10639414420396065 1106 2 0.20794676780598564 1106 3 0.15676474918084793 1106 4 0.11214904071978538 1106 5 0.1410755072831762 1106 6 0.2534483999872563 1106 7 0.24631771518789947 1106 8 0.2911209200944782 1106 9 0.2969067155766254 1106 10-11 0.25464385618419527 1106 12-13 0.31302451188921765 1106 14-15 0.4226505371387006 1106 16-17 0.3132231769930627 1106 18-19 0.2538963176446032 1106 20-21 0.19957120624190594 1106 22-23 0.2719224850847226 1106 24-25 0.29093284090824056 1106 26-27 0.36901334519951234 1106 28-29 0.37689282394205037 1106 30-31 0.3407513025926363 1106 32-33 0.2857397736501426 1106 34-35 0.295401565845097 1106 36-37 0.27266204757678025 1106 38-39 0.21893271499023825 1106 40-41 0.308119755688935 1106 42-43 0.22566423854662787 1106 44-45 0.288683836432611 1106 46-47 0.2817165551080194 1106 48-49 0.3393509897028508 1106 50-51 0.22948440424355 1106 52-53 0.21759407268789488 1106 54-55 0.33037338263152805 1106 56-57 0.3043058497666493 1106 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2216 114-115 -0.9195137484122675 2216 116-117 -0.9578264725781054 2216 118-119 -0.9916715186561511 2216 120-121 -0.9486912527761255 2216 122-123 -0.9023806402217858 2216 124-125 -0.8996865109862853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19171.0 3 3216.0 4 1849.0 5 1642.0 6 1668.0 7 1969.0 8 2109.0 9 2461.0 10 2711.0 11 3159.0 12 3673.0 13 3917.0 14 3826.0 15 3764.0 16 3329.0 17 3365.0 18 2653.0 19 2503.0 20 2338.0 21 2358.0 22 2355.0 23 3117.0 24 5410.0 25 9229.0 26 10643.0 27 8711.0 28 9176.0 29 11074.0 30 12445.0 31 15296.0 32 19959.0 33 26795.0 34 41342.0 35 75954.0 36 194849.0 37 439294.0 38 209232.0 39 984.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.433485464164704 17.994855087242435 13.318674388034735 24.252985060558125 2 15.734233924954513 18.934223625939786 40.271722338562945 25.05982011054276 3 19.7841436940248 23.384975729444562 27.57445226333852 29.256428313192114 4 13.139746352237875 15.599826111263068 35.60371888673481 35.65670864976425 5 14.883816161906916 37.42049948256129 32.611489195468465 15.084195160063327 6 34.063498210931826 34.72600180963218 16.8553699463965 14.355130033039496 7 30.85571614132614 31.389339191648475 19.398928641197617 18.356016025827767 8 26.72278244647196 36.078765567156324 18.647494251533352 18.550957734838367 9 26.62875185550752 17.52696756688617 18.900750606925683 36.94352997068062 10-11 25.883217401922455 26.728163513632207 25.867236219443484 21.521382865001854 12-13 26.020057806167273 23.453350885662967 26.723121995687883 23.803469312481873 14-15 23.21143216914402 25.081387604585377 24.13608786991689 27.571092356353716 16-17 23.803261385011268 28.185016580227867 24.688025695604924 23.323696339155937 18-19 23.89078629905612 27.027618779518942 25.953271864956385 23.128323056468556 20-21 24.42022968974935 26.31211288395154 25.95755910123641 23.3100983250627 22-23 24.34068539394246 25.981329887528304 25.78981846929772 23.88816624923151 24-25 24.106302553421276 26.224467930091627 25.740526455638697 23.928703060848395 26-27 23.80428418522914 26.605198878006554 25.77758094684151 23.81293598992279 28-29 23.758121089350183 26.64244080223729 25.90889818712874 23.690539921283786 30-31 23.957862223501355 26.293967395773375 25.769707809116575 23.978462571608695 32-33 23.78725953868602 26.342915168233517 25.910955275491137 23.958870017589323 34-35 24.156943900761405 26.512250213927974 25.635680873425738 23.695125011884887 36-37 24.34046066789693 26.286926643714498 25.494892721673352 23.87771996671522 38-39 23.87555538043399 26.380602736065057 25.78361427495818 23.960227608542773 40-41 24.05573531080766 26.167201649703532 25.798202542650532 23.978860496838283 42-43 23.868701391646013 26.111404490460032 25.681186160546606 24.338707957347353 44-45 23.842112746995962 26.31588414874408 25.821892228043186 24.020110876216776 46-47 24.01899853771738 26.50967616479334 25.63157845141958 23.839746846069698 48-49 24.14848839316041 26.2981855064096 25.660202312844344 23.893123787585647 50-51 23.963581277199367 26.441520907401483 25.70361261977786 23.89128519562129 52-53 24.17677680095735 26.285621037645416 25.625046578144634 23.912555583252598 54-55 24.02219825610829 26.19722303362461 25.691093542940703 24.0894851673264 56-57 23.90678545775702 26.51362484007876 25.733924461921504 23.845665240242717 58-59 24.132893074837806 26.111957524107066 25.678066757499607 24.07708264355552 60-61 24.040416413086167 26.27799218208286 25.808249028970494 23.873342375860478 62-63 23.91535270847004 26.417070291552232 25.864959420493467 23.802617579484263 64-65 23.695591828621758 26.33709334164956 25.77771856654752 24.189596263181162 66-67 23.882626284433616 26.443911566199414 25.545771842439386 24.127690306927587 68-69 23.75648739362602 26.1720789860924 26.055814611013417 24.015619009268164 70-71 23.694061417482708 26.108362778731802 26.02132233790967 24.17625346587582 72-73 23.74010497019391 26.2880349912068 25.702749492666904 24.269110545932385 74-75 23.67060335135714 26.6278066277638 25.627538947481128 24.074051073397936 76-77 24.020521948582914 26.330161987882818 25.62798036375535 24.021335699778916 78-79 23.87627587134479 26.33774540270824 25.77417532310937 24.011803402837597 80-81 23.83036683749628 26.60418857361914 25.7367053799549 23.828739208929683 82-83 23.973193308949856 26.476576056018892 25.653537310447298 23.89669332458395 84-85 23.97309676717473 26.367798014166898 25.565523840362385 24.09358137829598 86-87 23.886403473451363 26.348935193197025 25.879028900307905 23.885632433043707 88-89 23.811486101252907 26.520085106346524 25.76621627583914 23.902212516561423 90-91 23.96808915298496 26.551978493076046 25.75013793079815 23.729794423140845 92-93 23.78282119285301 26.238833629703816 25.869223720662642 24.10912145678053 94-95 23.73220990227092 26.340417602799725 25.973103621094744 23.954268873834607 96-97 23.95387569105503 26.347562662938202 25.781567924937264 23.9169937210695 98-99 23.71828840379736 26.402280026336992 25.69236351392395 24.187068055941698 100-101 23.572138988094984 26.369094072506066 25.920417913465254 24.138349025933696 102-103 23.63878584340704 26.022292093246403 26.017408736924168 24.321513326422384 104-105 23.476034577955247 26.26873433049867 26.015709230597235 24.23952186094885 106-107 23.60724320494384 26.317854711805825 25.858203952139775 24.216698131110558 108-109 23.700915700550755 26.31724582220726 25.74901289553783 24.23282558170415 110-111 23.506035140020387 26.443250837381033 25.947504133364173 24.103209889234407 112-113 23.513683581971915 26.468047083053296 26.069291539605903 23.948977795368883 114-115 23.487014975554704 26.541659796000094 25.755255792239605 24.216069436205593 116-117 23.333597449070275 26.457458449954707 26.07747442538766 24.131469675587354 118-119 23.463427255467746 26.670139341060032 25.769143117593433 24.09729028587878 120-121 23.460780618189293 26.73810054142965 25.91207765060654 23.889041189774517 122-123 23.34551052322511 26.722656897502155 25.893040326929494 24.038792252343242 124-125 23.472732163386006 26.799337985481596 25.74086860279643 23.98706124833597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 339.0 1 330.0 2 328.0 3 328.5 4 293.5 5 230.0 6 177.5 7 170.0 8 178.0 9 170.5 10 163.0 11 159.5 12 157.5 13 171.5 14 198.0 15 218.5 16 238.0 17 283.5 18 358.0 19 441.0 20 548.5 21 767.5 22 1076.0 23 1460.0 24 1944.5 25 2669.0 26 3585.0 27 4629.0 28 5780.5 29 7311.0 30 9180.5 31 10966.5 32 13138.0 33 15166.5 34 17036.5 35 18683.5 36 20976.0 37 23809.0 38 25450.5 39 26603.0 40 28011.0 41 30015.5 42 32270.5 43 34296.0 44 37172.5 45 39961.5 46 40799.0 47 41978.0 48 43658.5 49 44228.0 50 44517.5 51 44998.0 52 46078.0 53 48300.0 54 49876.0 55 48261.5 56 44065.5 57 40351.5 58 38411.0 59 35142.5 60 30284.5 61 25081.5 62 19982.0 63 15693.5 64 12128.0 65 9314.5 66 6815.0 67 5311.0 68 4032.5 69 2764.0 70 2064.5 71 1496.0 72 1151.5 73 929.5 74 695.5 75 526.0 76 386.5 77 268.0 78 176.5 79 111.5 80 75.0 81 60.0 82 46.0 83 33.0 84 24.5 85 23.5 86 23.0 87 14.0 88 11.5 89 14.0 90 10.5 91 9.5 92 11.0 93 8.0 94 5.0 95 4.0 96 4.0 97 4.5 98 3.5 99 2.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048649046804151615 2 0.20436025646955236 3 0.024667122323231806 4 0.10988860396078613 5 0.022097630414561826 6 0.039227576472361685 7 0.03682938402426971 8 0.09832589037177122 9 0.12187956620124604 10-11 0.046978877063516125 12-13 0.03164757534178525 14-15 0.011862487645026405 16-17 0.029335032623982266 18-19 0.051775261959700086 20-21 0.19515291046348493 22-23 0.1110877001848321 24-25 0.0750291637331634 26-27 0.013661131981095392 28-29 0.005224633547628959 30-31 0.007708475726009939 32-33 0.006552204367108448 34-35 0.008308023838032934 36-37 0.0038970627281494693 38-39 0.00882192221976693 40-41 0.005438757873351457 42-43 0.00989254384837942 44-45 0.01010666817410192 46-47 0.01653039794577687 48-49 0.016744522271499366 50-51 0.010663391420980415 52-53 0.013104408734216895 54-55 0.013447007655372894 56-57 0.014945877935430382 58-59 0.017258420653233363 60-61 0.008522148163755431 62-63 0.01781514390011186 64-65 0.029977405601149764 66-67 0.025694919086699797 68-69 0.03241842291438624 70-71 0.023339551503752314 72-73 0.014303504958262887 74-75 0.009292995736356426 76-77 0.009678419522656924 78-79 0.02188350608883933 80-81 0.017429720113811362 82-83 0.019314014180169346 84-85 0.015160002261152881 86-87 0.0250097212443878 88-89 0.025737743951844295 90-91 0.0251810207049658 92-93 0.03164757534178525 94-95 0.024709947188376304 96-97 0.02646576665930079 98-99 0.03139062615091825 100-101 0.01909988985444685 102-103 0.027065314771323783 104-105 0.02316825204317432 106-107 0.03044847911773926 108-109 0.020812884460226836 110-111 0.01850034174242385 112-113 0.024795596918665303 114-115 0.012290736296471403 116-117 0.011005990342136413 118-119 0.028949608837681773 120-121 0.02278282825687382 122-123 0.017087121192655364 124-125 0.017129946057799866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1167546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.573110750594225 #Duplication Level Percentage of deduplicated Percentage of total 1 78.32541169657094 45.87763013889138 2 12.158598380050826 14.24333858973425 3 3.8522790275245224 6.769198983641559 4 1.8372593255546479 4.304559758130979 5 0.9741447450719528 2.852934402010443 6 0.5849471413884064 2.055730421747198 7 0.4104906338467559 1.6830599350871358 8 0.3014040720831834 1.4123339275846711 9 0.2282980738712611 1.2034915528507846 >10 1.2131295657127545 12.856145586547886 >50 0.08263092911084308 3.3031226012184924 >100 0.03082210540197715 3.2159949052304473 >500 5.843038120656245E-4 0.22245919732474242 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.2847459543349898E-4 0.0 22-23 0.0 0.0 0.0 2.141243257224983E-4 0.0 24-25 0.0 0.0 0.0 3.425989211559973E-4 0.0 26-27 0.0 0.0 0.0 3.854237863004969E-4 0.0 28-29 0.0 0.0 0.0 9.849718983234923E-4 0.0 30-31 0.0 0.0 0.0 0.002954915694970477 0.0 32-33 0.0 0.0 0.0 0.007237402209420443 0.0 34-35 0.0 0.0 0.0 0.010877515746702912 0.0 36-37 0.0 0.0 0.0 0.01892859039386885 0.0 38-39 0.0 0.0 0.0 0.03434554184588873 0.0 40-41 0.0 0.0 0.0 0.05293153331860158 0.0 42-43 0.0 0.0 0.0 0.06967605559010095 0.0 44-45 0.0 0.0 0.0 0.08753402435535731 0.0 46-47 0.0 0.0 0.0 0.1125009207346006 0.0 48-49 0.0 0.0 0.0 0.1403799079436699 0.0 50-51 0.0 0.0 0.0 0.17275550599291162 0.0 52-53 0.0 0.0 0.0 0.2025616121334834 0.0 54-55 0.0 0.0 0.0 0.2363076058673491 0.0 56-57 0.0 0.0 0.0 0.2760490807214448 0.0 58-59 0.0 0.0 0.0 0.32062976533686893 0.0 60-61 0.0 0.0 0.0 0.37377542298119304 0.0 62-63 0.0 0.0 0.0 0.42542221034545963 0.0 64-65 0.0 0.0 0.0 0.47561295229481326 0.0 66-67 0.0 0.0 0.0 0.5374520575634707 0.0 68-69 0.0 0.0 0.0 0.6089695823547852 0.0 70-71 0.0 0.0 0.0 0.6886238315235544 0.0 72-73 4.282486514449966E-5 0.0 0.0 0.7771428277772354 0.0 74-75 8.564973028899932E-5 0.0 0.0 0.8633492813131132 0.0 76-77 8.564973028899932E-5 0.0 0.0 0.9583776570687579 0.0 78-79 8.564973028899932E-5 0.0 0.0 1.0727628718697164 0.0 80-81 8.564973028899932E-5 0.0 0.0 1.2047491062450644 0.0 82-83 8.564973028899932E-5 0.0 0.0 1.3442725168858445 0.0 84-85 8.564973028899932E-5 0.0 0.0 1.4861084702444272 0.0 86-87 8.564973028899932E-5 0.0 0.0 1.6343253285095405 0.0 88-89 8.564973028899932E-5 0.0 0.0 1.8063528117949956 0.0 90-91 8.564973028899932E-5 0.0 0.0 2.0003066260344347 0.0 92-93 8.564973028899932E-5 0.0 0.0 2.205951628458322 0.0 94-95 8.564973028899932E-5 0.0 0.0 2.4178918860584506 0.0 96-97 8.564973028899932E-5 0.0 0.0 2.6297464939282906 0.0 98-99 8.564973028899932E-5 0.0 0.0 2.8607009916525774 0.0 100-101 8.564973028899932E-5 0.0 0.0 3.1374780950814785 0.0 102-103 8.564973028899932E-5 0.0 0.0 3.4299719240184112 0.0 104-105 8.564973028899932E-5 0.0 0.0 3.730302703276787 0.0 106-107 8.564973028899932E-5 0.0 0.0 4.024338227358922 0.0 108-109 8.564973028899932E-5 0.0 0.0 4.336146070476024 0.0 110-111 8.564973028899932E-5 0.0 0.0 4.697587932295601 0.0 112-113 8.564973028899932E-5 0.0 0.0 5.082754769405231 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1805 0.0 48.275314 1 GGTATCA 1135 0.0 46.273914 1 TCAACGC 2095 0.0 40.9878 4 ATCAACG 2105 0.0 40.789577 3 CAACGCA 2140 0.0 40.37681 5 AACGCAG 2300 0.0 37.04981 6 TATCAAC 2365 0.0 36.338093 2 GTACATG 5045 0.0 35.84519 1 TACATGG 5255 0.0 33.16201 2 ACATGGG 5610 0.0 30.397789 3 ACGCAGA 2800 0.0 29.369654 7 CGCAGAG 2990 0.0 26.706154 8 CATGGGA 2370 0.0 26.670656 4 CATGGGG 3580 0.0 24.319016 4 ATGGGAT 1005 0.0 23.717651 5 GTCCTAC 230 2.2947825E-7 23.35415 1 GTGTACG 205 2.203602E-6 23.290863 1 GCAGAGT 3585 0.0 21.762941 9 GTGTAGC 800 0.0 21.635027 1 ATTCGCG 275 0.0 21.627066 80-81 >>END_MODULE