FastQCFastQC Report
Fri 27 May 2016
SRR940020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167546
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.3399437795170383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.26020388061797994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.1533986669475978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.064.94651
GTATCAA40000.060.7966771
TCAACGC49100.048.7900284
ATCAACG49300.048.59213
TATCAAC50200.047.7209242
CAACGCA50400.047.4806335
AACGCAG52350.045.8041346
ACGCAGA60750.039.0739367
CGCAGAG61900.038.155798
TATAACG1156.5777385E-736.2732732
GTGGTAT9450.034.69051
GCAGAGT71400.032.912399
ATAACGC1301.7153361E-632.0878943
ATACCGT953.2863748E-431.3157446
TAACGCA1352.3027296E-630.8994524
TGGTATC10850.030.2078272
GTACATG49900.029.6231231
GTACCGT1055.905757E-428.3332926
GGACCGA3150.028.333296
TACATGG51450.028.1454012
Produced by FastQC (version 0.11.5)