FastQCFastQC Report
Fri 27 May 2016
SRR940019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058562
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10670.10079711911064254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22150.043.3627851
GGTATCA14550.041.8217471
TCAACGC22400.040.453124
ATCAACG22550.040.182133
CAACGCA22900.039.798155
AACGCAG24150.037.7382056
TATCAAC26900.034.3783952
GTACATG50900.031.9970461
TACATGG50800.030.7711522
ACGCAGA30000.030.1806967
TAGATCG800.00533251929.7835855
GTCGCGC400.00537330829.73855660-61
ACATGGG51900.028.8298383
CGCAGAG31400.028.0762448
GTACCGT1159.981666E-425.8987676
GTGTAGC9950.025.1820581
CATGGGA31600.024.3365524
GCAGAGT38350.022.9750659
CATGGGG21200.021.652664
GTATAGG4203.274181E-1119.8858321
Produced by FastQC (version 0.11.5)