FastQCFastQC Report
Fri 27 May 2016
SRR940019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058562
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49110.46393125768731547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.33432146629106274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.21491419491725566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.10164732911251301No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.065.729581
GTATCAA43200.055.745471
CGTACGT350.00779941150.9944959
ATCAACG50800.046.4602743
TCAACGC51450.045.757474
TATCAAC53150.044.7445832
CAACGCA52500.044.5583465
AACGCAG55000.042.7312436
ATAACGC1700.042.070983
TAACGCA2050.040.7028164
ACGCAGA62450.037.439517
CGCAGAG63900.036.403748
TACATGG44550.033.848832
GTACATG46500.033.7143441
GTGGTAT10950.033.206851
GCAGAGT72200.032.0540169
TGGTATC11250.031.7884672
ACATGGG46700.031.1403313
TATAACG2351.0913936E-1130.4357662
GAGTACT47850.027.41553312-13
Produced by FastQC (version 0.11.5)