FastQCFastQC Report
Fri 27 May 2016
SRR940018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814575
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.053.383091
GTATCAA12950.046.993611
TCAACGC15000.039.753594
CAACGCA15400.038.690065
ATCAACG15900.037.8737563
AACGCAG15800.037.3334586
GTGGTAT4350.037.0324861
TATCAAC17400.035.6609152
GTACATG37350.034.823651
TACATGG38350.033.448872
ACATGGG38900.032.0339743
TATAGCG1004.380976E-429.8151934
ACGCAGA20400.028.915137
AACCGGT850.00716043528.0389147
CATGGGG27300.027.5217174
CGCAGAG22000.026.8122128
TGGTATC5350.025.649712
GTGTAAG3550.025.209911
GAGTACA27550.024.2551981
AGTACAT27500.023.865382
Produced by FastQC (version 0.11.5)