FastQCFastQC Report
Fri 27 May 2016
SRR940018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814575
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.34643832673480035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.25964459994475647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.165116778688273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.066.531791
GTATCAA29250.057.4902951
TCAACGC38000.043.9331474
ATCAACG38300.043.5890243
GTGGTAT6200.043.2804831
CAACGCA39000.042.88045
TATCAAC39600.042.3086362
AACGCAG40200.041.7381826
ACGCAGA46250.036.147517
CGCAGAG47100.035.242538
GTATAAC1901.0495569E-931.384681
GCAGAGT53200.031.0897319
CGCCTAT1004.3807633E-429.8154451
TAACGCA850.007136515328.0581444
GTACATG35200.027.7825741
GAGTACT32800.027.57106612-13
TGGTATC8900.027.4670312
GTACTTT33450.026.7685114-15
CAGAGTA51000.026.59796710-11
TACATGG36700.025.993922
Produced by FastQC (version 0.11.5)