##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940018_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 814575 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.12131111315717 34.0 33.0 34.0 31.0 34.0 2 33.172401559095235 34.0 33.0 34.0 31.0 34.0 3 33.23289813706534 34.0 34.0 34.0 31.0 34.0 4 36.47210754074211 37.0 37.0 37.0 35.0 37.0 5 36.46830555811313 37.0 37.0 37.0 35.0 37.0 6 36.54324279532271 37.0 37.0 37.0 35.0 37.0 7 36.5322554706442 37.0 37.0 37.0 35.0 37.0 8 36.53251695669521 37.0 37.0 37.0 35.0 37.0 9 38.402698339624955 39.0 39.0 39.0 37.0 39.0 10-11 38.38946935518522 39.0 39.0 39.0 37.0 39.0 12-13 38.36770340361538 39.0 39.0 39.0 37.0 39.0 14-15 40.00133014148482 41.0 40.0 41.0 38.0 41.0 16-17 39.99675965994537 41.0 40.0 41.0 38.0 41.0 18-19 40.01642574348587 41.0 40.0 41.0 38.0 41.0 20-21 40.005691311420065 41.0 40.0 41.0 38.0 41.0 22-23 39.96635239235184 41.0 40.0 41.0 38.0 41.0 24-25 39.933399011754595 41.0 40.0 41.0 38.0 41.0 26-27 39.844169659024644 41.0 40.0 41.0 37.5 41.0 28-29 39.76887395267471 41.0 40.0 41.0 37.0 41.0 30-31 39.64004173955744 41.0 40.0 41.0 37.0 41.0 32-33 39.492717674861126 41.0 40.0 41.0 35.5 41.0 34-35 39.39364944909922 41.0 40.0 41.0 35.0 41.0 36-37 39.29752324831968 41.0 40.0 41.0 35.0 41.0 38-39 39.17985698063407 41.0 39.0 41.0 35.0 41.0 40-41 39.05446705337139 41.0 39.0 41.0 35.0 41.0 42-43 38.8988853082896 41.0 39.0 41.0 35.0 41.0 44-45 38.699071294847 41.0 39.0 41.0 35.0 41.0 46-47 38.43125310744867 41.0 38.5 41.0 35.0 41.0 48-49 38.173109290120614 41.0 38.0 41.0 34.0 41.0 50-51 38.177614707055824 41.0 38.0 41.0 35.0 41.0 52-53 38.047858085504714 41.0 38.0 41.0 34.0 41.0 54-55 37.85918546481294 40.5 37.0 41.0 34.0 41.0 56-57 37.63599791302213 40.0 37.0 41.0 34.0 41.0 58-59 37.379289813706535 40.0 36.0 41.0 34.0 41.0 60-61 37.10598471595617 39.0 35.5 41.0 33.0 41.0 62-63 36.81039437743608 39.0 35.0 41.0 33.0 41.0 64-65 36.47527545038824 38.5 35.0 41.0 33.0 41.0 66-67 36.128186477611024 37.0 35.0 40.5 33.0 41.0 68-69 35.76019396617868 37.0 35.0 39.5 32.0 41.0 70-71 35.3840579443268 36.0 35.0 39.0 32.0 41.0 72-73 35.02582757879876 36.0 35.0 39.0 32.0 41.0 74-75 34.68223122487187 35.0 35.0 37.5 31.5 39.5 76-77 34.34382162477365 35.0 35.0 37.0 31.0 39.0 78-79 34.05073381824878 35.0 35.0 36.5 31.0 39.0 80-81 33.78619771046251 35.0 35.0 36.0 31.0 38.0 82-83 33.5547487953841 35.0 35.0 36.0 31.0 37.0 84-85 33.35504404137127 35.0 35.0 35.5 31.0 37.0 86-87 33.18698892060277 35.0 35.0 35.0 30.0 36.0 88-89 33.12148789245926 35.0 35.0 35.0 30.5 36.0 90-91 33.155099898720195 35.0 35.0 35.0 31.0 36.0 92-93 33.094604548384126 35.0 35.0 35.0 31.0 36.0 94-95 33.03670380259645 35.0 35.0 35.0 31.0 36.0 96-97 32.98691526256023 35.0 35.0 35.0 31.0 35.0 98-99 32.94329374213547 35.0 35.0 35.0 31.0 35.0 100-101 32.89006230242765 35.0 35.0 35.0 31.0 35.0 102-103 32.408020747015314 35.0 33.5 35.0 29.5 35.0 104-105 32.81202283399318 35.0 34.5 35.0 30.5 35.0 106-107 32.86240800417396 35.0 35.0 35.0 31.0 35.0 108-109 32.84525181843293 35.0 35.0 35.0 31.0 35.0 110-111 32.810094220912745 35.0 35.0 35.0 31.0 35.0 112-113 32.78615351563698 35.0 35.0 35.0 31.0 35.0 114-115 32.73352545806095 35.0 35.0 35.0 31.0 35.0 116-117 32.67863609857901 35.0 35.0 35.0 30.5 35.0 118-119 32.61875026854494 35.0 35.0 35.0 30.0 35.0 120-121 32.54888991191726 35.0 35.0 35.0 30.0 35.0 122-123 32.44921646257251 35.0 35.0 35.0 30.0 35.0 124-125 31.364502347850106 34.5 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16506623913183205 1101 2 0.14246693363412533 1101 3 0.12641617801399008 1101 4 0.06324364756591905 1101 5 0.06054785785254069 1101 6 0.11186133672791243 1101 7 0.11622201910125085 1101 8 0.1601619118596176 1101 9 0.15287234449563414 1101 10-11 0.1498181553005864 1101 12-13 0.18395016767864547 1101 14-15 0.2387866945717363 1101 16-17 0.27156835655048894 1101 18-19 0.24035173188900671 1101 20-21 0.24378513037952132 1101 22-23 0.23878440819331814 1101 24-25 0.19294703761858045 1101 26-27 0.2639533569226913 1101 28-29 0.34742250305280464 1101 30-31 0.3180058276369593 1101 32-33 0.2842437607966275 1101 34-35 0.27176434734651167 1101 36-37 0.27165159630025215 1101 38-39 0.2804161810759922 1101 40-41 0.2303608029773585 1101 42-43 0.41343880460979676 1101 44-45 0.4042080999793285 1101 46-47 0.34733469332258693 1101 48-49 0.3671525324494027 1101 50-51 0.27208775324542245 1101 52-53 0.2852263338255625 1101 54-55 0.12806199441118338 1101 56-57 0.18849848888773124 1101 58-59 0.3084328894211197 1101 60-61 0.2685303245503903 1101 62-63 0.25685987607159433 1101 64-65 0.23868448623325378 1101 66-67 0.24991418227184425 1101 68-69 0.21135058649310423 1101 70-71 0.2902032360283826 1101 72-73 0.23009264923635442 1101 74-75 0.29233160621171095 1101 76-77 0.32455906012119584 1101 78-79 0.3041278010180122 1101 80-81 0.2813923133802092 1101 82-83 0.36070336617271437 1101 84-85 0.32861911343066197 1101 86-87 0.2800938050933155 1101 88-89 0.36478645445236424 1101 90-91 0.3833939063162788 1101 92-93 0.3987518581916589 1101 94-95 0.3393012256057375 1101 96-97 0.3729107594348875 1101 98-99 0.3414169346576372 1101 100-101 0.2649416837108234 1101 102-103 0.23426410416823273 1101 104-105 0.22873894771120007 1101 106-107 0.29108795345452165 1101 108-109 0.26796550650693973 1101 110-111 0.3144501288859445 1101 112-113 0.2821935095755208 1101 114-115 0.32952088753623343 1101 116-117 0.33399039331997926 1101 118-119 0.3138816361783796 1101 120-121 0.31892925314080856 1101 122-123 0.29142486711661775 1101 124-125 0.3412772607501253 1102 1 0.1426444090176915 1102 2 0.16556399519272702 1102 3 0.17917900000758635 1102 4 0.07551035214031288 1102 5 0.08475253590336962 1102 6 0.13894759458339934 1102 7 0.1713066895705353 1102 8 0.15388048250126474 1102 9 0.19157969089528848 1102 10-11 0.1999259554083892 1102 12-13 0.19471092843940596 1102 14-15 0.28791126292707503 1102 16-17 0.26561121597796244 1102 18-19 0.3042351419262559 1102 20-21 0.26251984142192697 1102 22-23 0.2383120423363252 1102 24-25 0.1737799415284087 1102 26-27 0.17896156039243039 1102 28-29 0.17257013589274806 1102 30-31 0.0626321107247847 1102 32-33 0.10779364203881414 1102 34-35 0.19218772930835826 1102 36-37 0.18772447391159375 1102 38-39 0.23533411676316263 1102 40-41 0.14104219442798183 1102 42-43 0.08048306780790426 1102 44-45 0.05601967486782655 1102 46-47 0.04194794562814508 1102 48-49 -0.015465292476115167 1102 50-51 0.09864063749062524 1102 52-53 0.10549038485885376 1102 54-55 0.1436589523158318 1102 56-57 0.1926135506950999 1102 58-59 0.1118946351905521 1102 60-61 0.17527286590831892 1102 62-63 0.15697300695395455 1102 64-65 0.1602569462672534 1102 66-67 0.14667336749256776 1102 68-69 0.13370341269209263 1102 70-71 0.04235183433083023 1102 72-73 0.16610114293715128 1102 74-75 0.13698098932263747 1102 76-77 0.08363322304150955 1102 78-79 0.10652635341656236 1102 80-81 0.05852743666918059 1102 82-83 -0.015563433171003282 1102 84-85 -0.04118412175719044 1102 86-87 0.03126065241401221 1102 88-89 0.058132167028844606 1102 90-91 0.04363847040699653 1102 92-93 -0.04840239665490742 1102 94-95 -0.04848745064447968 1102 96-97 -0.038108247737966394 1102 98-99 0.016934167867177052 1102 100-101 0.0010224390223498858 1102 102-103 -0.039283111686671646 1102 104-105 -0.01345590986827716 1102 106-107 -0.021453878318084207 1102 108-109 0.03430729207948957 1102 110-111 -0.03562479811206032 1102 112-113 0.015896147124315974 1102 114-115 0.06163317734083762 1102 116-117 -0.013268107784675465 1102 118-119 -0.048230475933735306 1102 120-121 -0.025875610894829038 1102 122-123 -0.18900526715967203 1102 124-125 -0.12266153011174197 1103 1 0.07215993036994917 1103 2 0.06076240059521609 1103 3 0.04895256365870182 1103 4 0.03189822689553523 1103 5 0.040936124740021285 1103 6 0.040861169966362354 1103 7 0.09675692114321777 1103 8 0.1050357193707896 1103 9 0.12889893433698063 1103 10-11 0.16774070893742277 1103 12-13 0.14289088682344442 1103 14-15 0.15951026717914374 1103 16-17 0.18648690215977126 1103 18-19 0.1661830957109487 1103 20-21 0.18332256834710137 1103 22-23 0.15906807533725953 1103 24-25 0.08716271205403814 1103 26-27 0.13704063071639183 1103 28-29 0.1945337905094675 1103 30-31 0.14076417865277335 1103 32-33 0.09403352623787953 1103 34-35 0.1449393147130067 1103 36-37 0.12329707274950152 1103 38-39 0.19250265556435409 1103 40-41 0.18636594383336558 1103 42-43 0.17449106997916886 1103 44-45 0.11785264486405822 1103 46-47 -0.02916544181177727 1103 48-49 -0.0893730194450626 1103 50-51 -0.20578040162429545 1103 52-53 -0.17973361465087123 1103 54-55 -0.15530181603269 1103 56-57 -0.25427211047627907 1103 58-59 -0.2325372602617506 1103 60-61 -0.3440409085075018 1103 62-63 -0.2856211907422832 1103 64-65 -0.16719587098148736 1103 66-67 -0.19067126577043325 1103 68-69 -0.2613709619741229 1103 70-71 -0.3559546354042169 1103 72-73 -0.23645009634877567 1103 74-75 -0.19290137117197048 1103 76-77 -0.20295803293749515 1103 78-79 -0.23726787813276928 1103 80-81 -0.1562733699683605 1103 82-83 -0.11316394913887251 1103 84-85 -0.2292687078009621 1103 86-87 -0.07891074347166693 1103 88-89 -0.18978338700915742 1103 90-91 -0.12446257524506876 1103 92-93 -0.27986126076683604 1103 94-95 -0.20161142009497723 1103 96-97 -0.32669275060818137 1103 98-99 -0.3382154423844739 1103 100-101 -0.37533496794730326 1103 102-103 -0.3121171702350196 1103 104-105 -0.31503883390017506 1103 106-107 -0.23978737216178558 1103 108-109 -0.2248191813326912 1103 110-111 -0.2533585668662397 1103 112-113 -0.2770571560330666 1103 114-115 -0.3520925016909686 1103 116-117 -0.4479228791741434 1103 118-119 -0.39141476809761855 1103 120-121 -0.4274652471783398 1103 122-123 -0.3500234281778276 1103 124-125 -0.3375615613383047 1104 1 0.06029249188316754 1104 2 0.11531374494247615 1104 3 0.1515426680234242 1104 4 -0.06676235534978758 1104 5 0.017598425271003748 1104 6 0.01709530751372057 1104 7 0.06787282091927693 1104 8 0.0915485853867608 1104 9 0.14694375060717135 1104 10-11 0.12471596280709463 1104 12-13 0.14457818700231684 1104 14-15 0.16225952423185674 1104 16-17 0.1852221312410478 1104 18-19 0.12243159925314728 1104 20-21 0.1390485440375855 1104 22-23 0.1678285153310597 1104 24-25 0.13082829422893383 1104 26-27 0.17271618458183724 1104 28-29 0.12012638779683016 1104 30-31 0.17033981843248824 1104 32-33 0.2396864043663598 1104 34-35 0.023741021731218837 1104 36-37 -0.022860023629426962 1104 38-39 0.07370292034935488 1104 40-41 0.040815881158195566 1104 42-43 0.19360488527755138 1104 44-45 0.22785672931357226 1104 46-47 0.17111624436166295 1104 48-49 0.08476537297194398 1104 50-51 0.08308595237071614 1104 52-53 0.029553578487259813 1104 54-55 -0.013110860975722005 1104 56-57 -0.2034390236183441 1104 58-59 -0.09873773631137794 1104 60-61 -0.14564370283434158 1104 62-63 -0.018693964365184 1104 64-65 -0.11775149695859 1104 66-67 0.033481915170675336 1104 68-69 -0.027009474107757114 1104 70-71 -0.047820947146306025 1104 72-73 -0.046237282444749894 1104 74-75 0.0043831741161213245 1104 76-77 -0.13992511790812756 1104 78-79 -0.08585733461929834 1104 80-81 -0.10575858848641673 1104 82-83 -0.051908473863875315 1104 84-85 0.04543728225552002 1104 86-87 0.059628150346725306 1104 88-89 0.02129533019201091 1104 90-91 -0.08068150173906474 1104 92-93 -0.122224044389597 1104 94-95 -0.14411786366618884 1104 96-97 -0.015542203432787005 1104 98-99 -0.05769263284658166 1104 100-101 -0.05417188573719045 1104 102-103 -0.042177310233043386 1104 104-105 0.028481679895406842 1104 106-107 -0.029857934548225273 1104 108-109 -0.11580804064453787 1104 110-111 -0.11886891179096182 1104 112-113 -0.2094552912706007 1104 114-115 -0.1562107535465742 1104 116-117 -0.1294146891486463 1104 118-119 -0.08346135899071072 1104 120-121 -0.06537598082997675 1104 122-123 -0.08825669249804946 1104 124-125 0.02365870316501173 1105 1 0.17193565054602544 1105 2 0.19043590342983663 1105 3 0.1310228132280713 1105 4 -0.04058949531708578 1105 5 0.008490805721947936 1105 6 0.11046595807116688 1105 7 0.0938544763630631 1105 8 0.09431135314246575 1105 9 0.13136842800715698 1105 10-11 0.13427665409943046 1105 12-13 0.19717515174492917 1105 14-15 0.25102925663780695 1105 16-17 0.25607682499041573 1105 18-19 0.243492276690084 1105 20-21 0.18595139917470505 1105 22-23 0.2110342865690953 1105 24-25 0.1690294931833165 1105 26-27 0.18607048576139107 1105 28-29 0.14670105457433635 1105 30-31 0.13947089980067062 1105 32-33 0.09965213533133976 1105 34-35 0.13657666891717923 1105 36-37 0.15259374088411448 1105 38-39 0.03288166431070749 1105 40-41 -0.10346091288582926 1105 42-43 -0.08223175026063956 1105 44-45 -0.014632717486293245 1105 46-47 0.04236997377483931 1105 48-49 -0.15024538805927534 1105 50-51 -0.21157971670678677 1105 52-53 -0.19337157461496446 1105 54-55 -0.08434103620040645 1105 56-57 -0.012173932773066554 1105 58-59 -0.003935822418100088 1105 60-61 0.06781359018900446 1105 62-63 0.08899393801885935 1105 64-65 0.018587073717839075 1105 66-67 0.031042440198156385 1105 68-69 -0.02266949941696339 1105 70-71 0.02623342146289076 1105 72-73 0.10473767621995478 1105 74-75 0.1369618497241447 1105 76-77 0.059799159532495594 1105 78-79 0.014575415863333774 1105 80-81 -0.04397133361161565 1105 82-83 0.02558526811154138 1105 84-85 0.013155112314358064 1105 86-87 -0.008389418306805396 1105 88-89 0.06148016129462519 1105 90-91 0.019869481532843736 1105 92-93 -0.09030950452759612 1105 94-95 0.010960142263918726 1105 96-97 -0.05682438261088407 1105 98-99 0.014684308064737195 1105 100-101 0.04553253228961296 1105 102-103 0.0734137151592904 1105 104-105 0.05123641151620717 1105 106-107 -0.055799409317536686 1105 108-109 -0.0746357810127165 1105 110-111 -0.12416075280480499 1105 112-113 -0.07781324104013976 1105 114-115 0.04665500605290873 1105 116-117 -0.037605542733970765 1105 118-119 -0.014830919589627456 1105 120-121 0.05715244584260404 1105 122-123 -0.003100911561233488 1105 124-125 -0.051603856759612654 1106 1 0.08559017168176553 1106 2 0.045517187821978666 1106 3 0.05654368863987713 1106 4 -0.015690708074828308 1106 5 -0.016272432727227226 1106 6 0.007447079984295613 1106 7 0.08420268415667209 1106 8 0.11371637473152418 1106 9 0.017216595405429302 1106 10-11 0.09855416107884452 1106 12-13 0.05930228035470009 1106 14-15 0.0074660289607138 1106 16-17 0.11807917689662872 1106 18-19 0.1317260130791027 1106 20-21 0.18384680218550642 1106 22-23 0.11625336393962726 1106 24-25 0.1535131959095537 1106 26-27 0.07839761616651231 1106 28-29 0.06629907610056307 1106 30-31 0.07143528009133604 1106 32-33 0.1114085245269365 1106 34-35 0.021801850471774742 1106 36-37 0.08373315865267728 1106 38-39 -0.06218875822450798 1106 40-41 -0.020080900497930543 1106 42-43 -0.03753072062841767 1106 44-45 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88-89 -0.4458269588548234 2216 90-91 -0.40651753579056304 2216 92-93 -0.29766560190498836 2216 94-95 -0.4076744123464735 2216 96-97 -0.3433090198605555 2216 98-99 -0.29958902563797096 2216 100-101 -0.3205662354914267 2216 102-103 -0.39283485916953254 2216 104-105 -0.3960498214397674 2216 106-107 -0.43793077073811304 2216 108-109 -0.3332368512984374 2216 110-111 -0.3487263085891925 2216 112-113 -0.4040533266797226 2216 114-115 -0.31527024330233644 2216 116-117 -0.4015604460496718 2216 118-119 -0.3583891857643948 2216 120-121 -0.40988619337451837 2216 122-123 -0.49884271532406643 2216 124-125 -0.6263916133934941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 5.0 4 8.0 5 35.0 6 67.0 7 205.0 8 313.0 9 567.0 10 952.0 11 1386.0 12 1825.0 13 2801.0 14 4945.0 15 5200.0 16 2575.0 17 2140.0 18 2084.0 19 1734.0 20 1480.0 21 1361.0 22 1466.0 23 1528.0 24 1931.0 25 2532.0 26 3134.0 27 3950.0 28 4889.0 29 5962.0 30 7281.0 31 9283.0 32 12020.0 33 15967.0 34 23715.0 35 44024.0 36 121913.0 37 345985.0 38 178101.0 39 1203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08736171309996 17.414633547388974 13.61578036172538 23.88222437778569 2 16.291246498083613 18.642349064641508 39.914849107609804 25.151555329665072 3 20.26988373049438 22.979647005240782 27.915852845601314 28.834616418663522 4 13.504808525651821 15.345853653139525 34.864792101798386 36.28454571941027 5 14.988750853510568 36.389638013273014 32.486331550171684 16.13527958304474 6 34.32463458504454 34.244215670024616 16.260213509149953 15.170936235780893 7 30.617684068379212 31.402756038424943 19.00033759936163 18.97922229383421 8 27.57511585796274 34.575330693920144 18.27038639781481 19.579167050302303 9 27.099407666574592 16.238283767608877 18.935150231715923 37.7271583341006 10-11 26.6087837215726 25.63975079028942 25.46886413160237 22.28260135653562 12-13 27.020654942761563 22.609520301997975 26.713500905380105 23.656323849860357 14-15 23.713961268145965 23.899149863425713 24.797225547064418 27.5896633213639 16-17 23.59322346008655 27.348187705245063 25.241383543565664 23.817205291102724 18-19 23.642697111990916 25.491575361384772 27.687505754534573 23.178221772089742 20-21 24.08900346806617 24.653837890924716 28.0811466101955 23.176012030813613 22-23 23.935303685971213 24.36669428843262 28.074885676579814 23.623116349016357 24-25 23.726298990271 24.695761593468987 27.92284320044195 23.655096215818062 26-27 23.433876561397046 25.049995396372342 27.957708007243042 23.55842003498757 28-29 23.354954028145983 25.086655671151014 28.080918319932675 23.47747198077033 30-31 23.497005796895067 24.813829068936645 28.032321188744053 23.65684394542423 32-33 23.439769204800047 24.905871159807262 27.893379983426943 23.760979651965748 34-35 23.8268685886576 24.96623993950197 27.729347436445153 23.477544035395276 36-37 23.945283846949454 24.78321317754514 27.532893222476957 23.73860975302845 38-39 23.62999992634078 24.82702360286878 27.738772493683722 23.804203977106713 40-41 23.70733204431759 24.66194027560384 27.785716477917934 23.845011202160634 42-43 23.80845984761201 24.780880113385603 27.41001184066843 24.00064819833396 44-45 23.751073419763447 25.03727429028462 27.39618902991089 23.81546326004104 46-47 24.010864561274285 25.339717030353253 26.92637264831354 23.723045760058923 48-49 24.209575207289316 25.328888408739914 26.62553617772496 23.83600020624581 50-51 24.14415608888954 25.579775130545702 26.484556414709022 23.79151236585574 52-53 24.257619003774973 25.430991621397663 26.44759537181966 23.863794003007705 54-55 24.055182150201023 25.368505048645 26.48804591351318 24.088266887640795 56-57 24.018156930386155 25.717652980425136 26.52906766188088 23.73512242730783 58-59 24.23798425297382 25.258158787526863 26.538226160786138 23.965630798713182 60-61 23.988867739339614 25.42484097428151 26.82085445133902 23.765436835039857 62-63 23.817295911728092 25.45354995371814 26.925730504533657 23.80342363002011 64-65 23.753677569602974 25.30118780971228 26.833659375876618 24.11147524480813 66-67 23.784298729024233 25.589482070930586 26.576566069747145 24.049653130298037 68-69 23.75857662150168 25.337387010799578 26.88010469340228 24.02393167429647 70-71 23.719776813391196 25.441493312381454 26.724018341077755 24.114711533149595 72-73 23.70377190559494 25.587085289875088 26.4103366786361 24.298806125893872 74-75 23.752017923457018 25.971703035325174 26.176042721664672 24.100236319553144 76-77 24.004822276757928 25.728160107445436 26.220147737470732 24.046869878325904 78-79 23.90207342651548 25.639371471679212 26.392284513476138 24.06627058832917 80-81 23.874597182579873 25.925728140441333 26.208390878679065 23.991283798299726 82-83 24.03148881318479 25.811189884295494 26.226130190590187 23.931191111929532 84-85 24.00581408553065 25.72972236391191 26.100284381949574 24.164179168607866 86-87 24.054106809203336 25.69766528271219 26.277907225126263 23.97032068295821 88-89 23.937390663843107 25.795967222171072 26.115458981677563 24.151183132308258 90-91 23.93605495875454 25.901375503406992 26.143342321666097 24.01922721617237 92-93 23.87465303822503 25.625513307004542 26.307283220247225 24.192550434523202 94-95 23.82540427895785 25.6458181795861 26.26970754418756 24.25906999726849 96-97 23.96244655939158 25.561506188829107 26.239960224534954 24.236087027244352 98-99 23.843538041309888 25.61912653837891 25.986373262130556 24.55096215818065 100-101 23.69724770079066 25.44178026906105 26.334333243102066 24.526638787046227 102-103 23.723168523463155 25.162385292944172 26.402172912254855 24.712273271337814 104-105 23.695485375809472 25.41521652395421 26.26940429058098 24.61989380965534 106-107 23.63479002789807 25.462619963232246 26.207918877693515 24.694671131176168 108-109 23.833281567843688 25.223031509837696 26.1933582216897 24.75032870062892 110-111 23.616425743485866 25.477273424792067 26.249762145904302 24.656538685817758 112-113 23.567132718614538 25.540375141331715 26.28641018054858 24.606081959505165 114-115 23.486133336770763 25.572838883251908 26.13277204974701 24.808255730230314 116-117 23.345049111741027 25.51385944257775 26.403775729801332 24.737315715879895 118-119 23.396169897498506 25.641148404655933 26.10040714557986 24.862274552265696 120-121 23.26053463462542 25.726483135377343 26.29119479483166 24.721787435165577 122-123 23.283307246109935 25.675659086026453 26.18015529570635 24.86087837215726 124-125 23.362708768251206 25.713152775791386 26.09758897184341 24.826549484114004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 45.0 2 134.5 3 241.0 4 154.5 5 69.0 6 223.5 7 601.0 8 1068.5 9 1168.5 10 843.0 11 584.5 12 542.0 13 570.0 14 600.5 15 691.5 16 777.5 17 848.5 18 895.5 19 957.0 20 1050.0 21 1148.5 22 1289.5 23 1506.5 24 1853.5 25 2256.0 26 2777.0 27 3435.0 28 3990.5 29 4810.5 30 5890.0 31 6896.0 32 8348.5 33 9497.0 34 10230.0 35 11095.0 36 12236.5 37 13693.0 38 15026.0 39 16080.0 40 17054.0 41 18709.0 42 20502.5 43 22093.0 44 24299.5 45 26467.5 46 27944.0 47 29524.5 48 30964.5 49 31759.0 50 31967.0 51 32404.0 52 33790.0 53 35555.0 54 36549.5 55 35366.0 56 32287.5 57 29487.5 58 27817.5 59 25237.0 60 21758.0 61 18336.5 62 14742.0 63 11385.5 64 8770.5 65 6593.5 66 4832.5 67 3953.5 68 2965.0 69 1992.5 70 1475.5 71 1060.0 72 788.0 73 617.0 74 477.5 75 370.0 76 243.5 77 124.5 78 67.0 79 31.0 80 19.5 81 15.5 82 9.5 83 6.5 84 3.0 85 1.5 86 2.5 87 3.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017800693613233894 2 0.002578031488813185 3 2.4552680845839857E-4 4 0.13798606635361998 5 0.03572415063069699 6 0.011048706380627936 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.138170211459964E-5 30-31 1.2276340422919928E-4 32-33 0.0 34-35 3.6829021268759785E-4 36-37 9.207255317189945E-4 38-39 0.0015959242549795905 40-41 0.0 42-43 0.001166252340177393 44-45 6.75198723260596E-4 46-47 0.0 48-49 0.0015959242549795905 50-51 0.0016573059570941901 52-53 0.0 54-55 0.0 56-57 0.0012276340422919928 58-59 0.001043488935948194 60-61 6.75198723260596E-4 62-63 1.2276340422919928E-4 64-65 0.001043488935948194 66-67 4.910536169167971E-4 68-69 7.365804253751957E-4 70-71 0.0012276340422919928 72-73 0.0 74-75 0.0 76-77 0.002700794893042384 78-79 6.75198723260596E-4 80-81 0.0 82-83 0.0 84-85 1.8414510634379893E-4 86-87 1.2276340422919928E-4 88-89 0.0 90-91 6.138170211459964E-5 92-93 4.910536169167971E-4 94-95 9.207255317189945E-4 96-97 3.069085105729982E-4 98-99 0.0 100-101 1.8414510634379893E-4 102-103 0.0 104-105 0.0 106-107 3.069085105729982E-4 108-109 4.910536169167971E-4 110-111 0.0 112-113 2.4552680845839857E-4 114-115 0.0016573059570941901 116-117 4.910536169167971E-4 118-119 1.8414510634379893E-4 120-121 0.0 122-123 0.0 124-125 6.75198723260596E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 814575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.4651798568324 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53313995007912 50.30517071417145 2 11.383381921785826 14.22129998246732 3 3.3735345558217062 6.321853283479262 4 1.4947471295340207 3.7347859314730614 5 0.803598712011165 2.5098469039248137 6 0.5204840624507222 1.9507278344159484 7 0.3536843189597269 1.5465068217452433 8 0.2564814485601533 1.2816927851400708 9 0.18772010793688473 1.0553373274519346 >10 1.0179182950011152 11.24237025460615 >50 0.0528944831779361 2.27597218135912 >100 0.02123527707381087 2.568921320712722 >500 5.898688039662303E-4 0.2139539551433186 >1k 5.898688039662303E-4 0.7715607039094877 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.34643832673480035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2115 0.25964459994475647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.165116778688273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.2276340422919928E-4 0.0 4 0.0 0.0 0.0 1.2276340422919928E-4 0.0 5 0.0 0.0 0.0 1.2276340422919928E-4 0.0 6 0.0 0.0 0.0 1.2276340422919928E-4 0.0 7 0.0 0.0 0.0 1.2276340422919928E-4 0.0 8 0.0 0.0 0.0 1.2276340422919928E-4 0.0 9 0.0 0.0 0.0 1.2276340422919928E-4 0.0 10-11 0.0 0.0 0.0 1.2276340422919928E-4 0.0 12-13 0.0 0.0 0.0 1.2276340422919928E-4 0.0 14-15 0.0 0.0 0.0 1.2276340422919928E-4 0.0 16-17 0.0 0.0 0.0 1.2276340422919928E-4 0.0 18-19 0.0 0.0 0.0 1.2276340422919928E-4 0.0 20-21 0.0 0.0 0.0 1.2276340422919928E-4 0.0 22-23 0.0 0.0 0.0 1.2276340422919928E-4 0.0 24-25 0.0 0.0 0.0 1.2276340422919928E-4 0.0 26-27 0.0 0.0 0.0 2.4552680845839857E-4 0.0 28-29 0.0 0.0 0.0 6.751987232605961E-4 0.0 30-31 0.0 0.0 0.0 0.002025596169781788 0.0 32-33 0.0 0.0 0.0 0.0054015897860847685 0.0 34-35 0.0 0.0 0.0 0.00847067489181475 0.0 36-37 0.0 0.0 0.0 0.015099898720191511 0.0 38-39 0.0 0.0 0.0 0.028235582972715834 0.0 40-41 0.0 0.0 0.0 0.04327409999079274 0.0 42-43 0.0 0.0 0.0 0.057084982966577665 0.0 44-45 0.0 0.0 0.0 0.07310560721848816 0.0 46-47 0.0 0.0 0.0 0.09219531657612866 1.8414510634379893E-4 48-49 0.0 0.0 0.0 0.11404720252892613 2.4552680845839857E-4 50-51 0.0 0.0 0.0 0.14111653316146455 2.4552680845839857E-4 52-53 0.0 0.0 0.0 0.1680017186876592 2.4552680845839857E-4 54-55 0.0 0.0 0.0 0.19421170549059324 2.4552680845839857E-4 56-57 0.0 0.0 0.0 0.22797164165362305 2.4552680845839857E-4 58-59 0.0 0.0 0.0 0.26602829696467484 2.4552680845839857E-4 60-61 0.0 0.0 0.0 0.30991621397661356 2.4552680845839857E-4 62-63 0.0 0.0 0.0 0.3552772918393027 2.4552680845839857E-4 64-65 0.0 0.0 0.0 0.3994721173618144 2.4552680845839857E-4 66-67 0.0 0.0 0.0 0.4535493969247767 2.4552680845839857E-4 68-69 0.0 0.0 0.0 0.5173863671239604 2.4552680845839857E-4 70-71 0.0 0.0 0.0 0.59104440966148 3.6829021268759785E-4 72-73 0.0 0.0 0.0 0.6615719853911549 3.6829021268759785E-4 74-75 0.0 0.0 0.0 0.7338182487800387 3.6829021268759785E-4 76-77 0.0 0.0 0.0 0.821164410889114 3.6829021268759785E-4 78-79 0.0 0.0 0.0 0.9159991406561704 3.6829021268759785E-4 80-81 0.0 0.0 0.0 1.0275910751005126 4.296719148021975E-4 82-83 0.0 0.0 0.0 1.1488813184789615 4.910536169167971E-4 84-85 0.0 0.0 0.0 1.272074394622963 4.910536169167971E-4 86-87 0.0 0.0 0.0 1.4044133443820397 4.910536169167971E-4 88-89 0.0 0.0 0.0 1.5520977196697665 4.910536169167971E-4 90-91 0.0 0.0 0.0 1.7269128072921462 4.910536169167971E-4 92-93 0.0 0.0 0.0 1.912469692784581 4.910536169167971E-4 94-95 0.0 0.0 0.0 2.1015253352975476 6.138170211459964E-4 96-97 0.0 0.0 0.0 2.289783015683025 6.138170211459964E-4 98-99 0.0 0.0 0.0 2.493815793511954 6.138170211459964E-4 100-101 0.0 0.0 0.0 2.744805573458552 6.138170211459964E-4 102-103 0.0 0.0 0.0 3.004450173403309 6.138170211459964E-4 104-105 0.0 0.0 0.0 3.269189454623577 6.138170211459964E-4 106-107 0.0 0.0 0.0 3.52932510818525 6.751987232605961E-4 108-109 0.0 0.0 0.0 3.804069606850198 7.365804253751957E-4 110-111 0.0 0.0 0.0 4.113249240401437 7.365804253751957E-4 112-113 0.0 0.0 0.0 4.454040450541694 7.365804253751957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1685 0.0 66.53179 1 GTATCAA 2925 0.0 57.490295 1 TCAACGC 3800 0.0 43.933147 4 ATCAACG 3830 0.0 43.589024 3 GTGGTAT 620 0.0 43.280483 1 CAACGCA 3900 0.0 42.8804 5 TATCAAC 3960 0.0 42.308636 2 AACGCAG 4020 0.0 41.738182 6 ACGCAGA 4625 0.0 36.14751 7 CGCAGAG 4710 0.0 35.24253 8 GTATAAC 190 1.0495569E-9 31.38468 1 GCAGAGT 5320 0.0 31.089731 9 CGCCTAT 100 4.3807633E-4 29.815445 1 TAACGCA 85 0.0071365153 28.058144 4 GTACATG 3520 0.0 27.782574 1 GAGTACT 3280 0.0 27.571066 12-13 TGGTATC 890 0.0 27.467031 2 GTACTTT 3345 0.0 26.76851 14-15 CAGAGTA 5100 0.0 26.597967 10-11 TACATGG 3670 0.0 25.99392 2 >>END_MODULE