FastQCFastQC Report
Fri 27 May 2016
SRR940017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656345
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.050.8992921
GGTATCA16950.049.654451
TCAACGC30700.041.1774024
ATCAACG30900.040.7154353
CAACGCA31450.039.9658935
AACGCAG32600.038.556056
TATCAAC33600.038.3681222
GTACATG76700.033.8534281
TACATGG77500.032.960832
ACGCAGA40850.030.6235077
ACATGGG79150.030.5851233
CGCAGAG44050.027.9931728
CATGGGG50800.025.4716684
GAGTACA59450.023.996881
AGTACAT56850.023.516552
CATGGGA38150.023.132824
GCAGAGT52350.022.7487289
ATGGGGA28900.021.2308335
GTCCAAG16650.020.7932551
GTGGTAT10150.020.58311
Produced by FastQC (version 0.11.5)