FastQCFastQC Report
Fri 27 May 2016
SRR940017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656345
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.22682472552517743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.1668734472588742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.10040178827478576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.068.0752641
GTATCAA52800.060.3226781
TCAACGC66400.047.5922744
ATCAACG66750.047.342733
TATCAAC67150.047.062142
CAACGCA68650.045.9545825
AACGCAG71100.044.35236
GTGGTAT11200.038.8756871
ACGCAGA81850.038.1602487
CGCAGAG84250.037.0731938
GTACATG78350.033.1149061
GCAGAGT94300.032.6804969
TACATGG80700.031.3281062
ACATGGG81000.030.7694663
TGGTATC14250.028.8719442
GAGTACT52750.027.6885112-13
CAGAGTA90050.027.21974810-11
GTACTTT54700.026.37515414-15
CATGGGG49750.025.6477584
GTATAGG5600.025.5620961
Produced by FastQC (version 0.11.5)