FastQCFastQC Report
Fri 27 May 2016
SRR940016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885286
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26400.050.4115261
TCAACGC29300.044.3610424
CAACGCA29600.043.87415
ATCAACG29900.043.667943
TATCAAC32050.040.9616362
AACGCAG31850.040.7746736
GGTATCA17750.038.3296931
GTACATG67200.032.5930981
ACGCAGA39150.032.4109157
TACATGG66200.032.0903632
CGCAGAG40000.031.7221838
ACATGGG69400.029.6380333
GTACGGT900.00943756526.4976433
GTGGTAT10600.024.2098141
CATGGGG49550.024.1860684
GCAGAGT52150.023.9785989
GTATAGC9400.020.3165971
GTATTAG6900.019.8933351
CATGGGA27600.019.6582644
AGAGTAC46050.019.44398110-11
Produced by FastQC (version 0.11.5)