FastQCFastQC Report
Fri 27 May 2016
SRR940016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885286
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.21646583064850639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29280.15530800101416972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.063.0554431
GTATCAA52100.056.199261
ATCAACG62800.045.5757453
TCAACGC63350.045.1800654
TATCAAC64250.044.6399962
CAACGCA64350.044.404785
AACGCAG67700.042.1884656
ACGCAGA77800.036.5527767
CGCAGAG79100.035.9520348
GCAGAGT90450.031.2433159
GTACATG67450.029.4407651
TACATGG64900.029.308812
GTGGTAT13650.029.2704241
ACATGGG66550.027.3277573
GGACCGA3300.027.0469636
GAGTACT56450.025.87376212-13
CAGAGTA87850.025.53122310-11
AGAGTAC84250.024.962710-11
GTACTTT59600.024.45636414-15
CATGGGG47100.023.420874
Produced by FastQC (version 0.11.5)