FastQCFastQC Report
Fri 27 May 2016
SRR940015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543608
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21300.046.215321
GGTATCA13750.045.9922641
TCAACGC23500.040.8454134
ATCAACG23700.040.4954643
CAACGCA23950.040.3007135
AACGCAG24750.038.757336
TATCAAC26700.035.3008462
GTACATG54750.033.7799641
TACATGG55750.031.7797782
ACGCAGA30100.031.6706287
CGCAGAG31750.029.8370978
ACATGGG57250.028.9466383
TTACGCG450.00955008726.4338280-81
CATGGGG35600.025.6228074
CTATACG1451.3222966E-424.6699644
GCAGAGT38800.024.0991619
TACGCGA650.002057739322.87542380-81
GTGTAGC11750.021.8329411
TAGCCCT11850.021.1308124
GAGTACA42550.020.891441
Produced by FastQC (version 0.11.5)