FastQCFastQC Report
Fri 27 May 2016
SRR940015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543608
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.2181901104425476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.14848329368596172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.060.2268751
GTATCAA38000.059.4706881
ATCAACG46350.048.2267383
TCAACGC47000.047.5597764
CAACGCA48200.046.5595865
AACGCAG49300.045.7399866
TATCAAC49600.044.9494632
ACGCAGA58750.038.177647
CGCAGAG59700.037.4704678
GTACATG53450.031.9055061
GCAGAGT69800.031.8780529
TACATGG55750.030.1535742
ACATGGG55350.029.2925823
TGGTATC11600.025.180982
AGAGTAC67550.024.88104810-11
CATGGGG35550.024.4804634
GAGTACT43900.023.58089612-13
GTGGTAT12450.023.467891
AGTACTT46750.023.35232212-13
CAGAGTA71000.023.21116810-11
Produced by FastQC (version 0.11.5)