FastQCFastQC Report
Fri 27 May 2016
SRR940014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529731
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.047.3171731
GGTATCA13000.043.6029931
CAACGCA21550.043.4015465
TCAACGC21750.043.0390974
ATCAACG21700.042.8592873
AACGCAG23700.039.7156376
TATCAAC25050.037.155612
ACGCAGA28650.033.2696467
GTACATG55550.032.6531831
CGCAGAG29100.032.5504468
TACATGG54250.032.3336832
ACATGGG56700.030.072053
GGACCGT1254.842745E-528.5952596
TTAGCGC850.0071414828.05573
CATGGGA29050.023.3981484
GCAGAGT40000.023.074899
GAGTACA41600.022.8055111
GGACCGA2704.485446E-822.0642436
AGTACAT40900.021.5896662
CATGGGG33700.021.5850324
Produced by FastQC (version 0.11.5)