FastQCFastQC Report
Fri 27 May 2016
SRR940014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529731
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.2430492681393003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.16225074866103908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.10563948824989491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.067.215191
GTATCAA42350.059.3921051
ATCAACG49600.049.7429473
TCAACGC49850.049.2543834
CAACGCA51050.048.032115
TATCAAC52000.047.7909362
AACGCAG53750.045.6074036
ACGCAGA63600.038.633767
CGCAGAG65150.037.349348
GCAGAGT77800.030.9705879
GTACATG57500.030.8900131
GGACCGA2950.030.2542136
TACATGG57550.029.9271472
ACATGGG56050.029.1331713
GAGTACT50500.025.44698712-13
TGGTATC11150.025.1209352
GTGGTAT11000.024.924981
GTACTTT52100.024.72260514-15
CAGAGTA76050.024.6816510-11
AGAGTAC73450.024.66434110-11
Produced by FastQC (version 0.11.5)