FastQCFastQC Report
Fri 27 May 2016
SRR940013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898794
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA14350.048.5883455
TCAACGC14450.047.8717234
GTATCAA14850.047.44631
ACCGTAT653.487114E-545.8410728
GGTATCA8150.045.4235271
ATCAACG15400.045.30333
AACGCAG15550.044.4555246
TATCAAC17450.040.021342
GTACATG36850.035.6477361
TACATGG37450.034.108542
ACGCAGA20250.033.5488787
ACATGGG36700.032.820863
CGCAGAG21300.031.6152748
CATGGGG23700.029.187614
GCAGAGT25900.025.7643689
CATGGGA18600.023.404474
GTGTAGC6650.021.549461
ATGGGAG11750.021.301475
GAGTACA26450.020.5429331
GTCTAGG3501.4679244E-920.4719871
Produced by FastQC (version 0.11.5)