##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 898794 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60852097366026 34.0 31.0 34.0 30.0 34.0 2 31.734554302765705 34.0 31.0 34.0 30.0 34.0 3 32.049266016462056 34.0 31.0 34.0 30.0 34.0 4 35.23579485399324 37.0 35.0 37.0 33.0 37.0 5 35.350194816609815 37.0 37.0 37.0 35.0 37.0 6 35.33930689345946 37.0 37.0 37.0 35.0 37.0 7 35.27754190615425 37.0 37.0 37.0 33.0 37.0 8 35.32303175143581 37.0 37.0 37.0 35.0 37.0 9 37.01412225715792 39.0 39.0 39.0 35.0 39.0 10-11 37.014497760332176 39.0 39.0 39.0 35.0 39.0 12-13 36.987668475757516 39.0 39.0 39.0 35.0 39.0 14-15 38.52426084286277 41.0 40.0 41.0 35.0 41.0 16-17 38.56531474397915 41.0 40.0 41.0 35.0 41.0 18-19 38.57032089666821 41.0 40.0 41.0 35.0 41.0 20-21 38.55087150114487 41.0 40.0 41.0 35.0 41.0 22-23 38.581208819818556 41.0 40.0 41.0 35.0 41.0 24-25 38.61386758256063 41.0 40.0 41.0 35.0 41.0 26-27 38.63021782521913 41.0 40.0 41.0 35.0 41.0 28-29 38.589032080766 41.0 40.0 41.0 34.0 41.0 30-31 38.53372074134896 41.0 40.0 41.0 34.0 41.0 32-33 38.48136391653705 41.0 39.0 41.0 34.0 41.0 34-35 38.41690977020318 41.0 39.0 41.0 34.0 41.0 36-37 38.299516351911564 41.0 39.0 41.0 33.5 41.0 38-39 38.20891160822168 41.0 39.0 41.0 33.0 41.0 40-41 38.11139259941655 41.0 39.0 41.0 33.0 41.0 42-43 37.97671212758429 41.0 38.5 41.0 33.0 41.0 44-45 37.826944216361035 40.0 38.0 41.0 33.0 41.0 46-47 37.66310856547774 40.0 38.0 41.0 32.0 41.0 48-49 37.52286842146253 40.0 38.0 41.0 32.0 41.0 50-51 37.3532199814418 40.0 37.5 41.0 31.5 41.0 52-53 37.48220726885137 40.0 37.5 41.0 32.0 41.0 54-55 37.280563733180244 40.0 37.0 41.0 31.0 41.0 56-57 37.16561414517676 40.0 37.0 41.0 31.0 41.0 58-59 36.97629935224312 40.0 36.0 41.0 31.0 41.0 60-61 36.73625380231733 39.5 36.0 41.0 31.0 41.0 62-63 36.45023164373594 39.0 35.0 41.0 31.0 41.0 64-65 36.1457280533693 39.0 35.0 41.0 31.0 41.0 66-67 35.81440686074896 38.0 35.0 41.0 30.5 41.0 68-69 35.456402134415676 37.0 35.0 40.0 30.0 41.0 70-71 35.0831469724987 37.0 35.0 39.5 30.0 41.0 72-73 34.69978827183982 36.0 35.0 39.0 29.5 41.0 74-75 34.32230132822427 36.0 35.0 39.0 29.0 40.5 76-77 33.950019136754364 35.0 34.5 37.0 29.0 39.5 78-79 33.60763367356702 35.0 34.0 37.0 29.0 39.0 80-81 33.28767937925709 35.0 34.0 36.5 29.0 39.0 82-83 32.98469560321942 35.0 34.0 36.0 28.5 37.5 84-85 32.74699875611097 35.0 34.0 36.0 28.0 37.0 86-87 32.54203410347644 35.0 34.0 35.5 28.0 37.0 88-89 32.369485110047464 35.0 34.0 35.0 27.0 36.0 90-91 32.22190568695385 35.0 34.0 35.0 27.0 36.0 92-93 32.07448258444093 35.0 34.0 35.0 27.0 36.0 94-95 31.958439308673622 35.0 34.0 35.0 27.0 36.0 96-97 31.857232024245825 35.0 33.5 35.0 26.0 35.0 98-99 31.777938548766457 35.0 33.0 35.0 25.5 35.0 100-101 31.684568989112076 35.0 33.0 35.0 25.0 35.0 102-103 30.991916390185068 34.0 32.0 35.0 24.5 35.0 104-105 31.8969819558208 35.0 34.0 35.0 27.0 35.0 106-107 32.00474024081157 35.0 34.0 35.0 27.0 35.0 108-109 31.988940736141984 35.0 34.0 35.0 27.0 35.0 110-111 31.94122679946684 35.0 34.0 35.0 27.0 35.0 112-113 31.886512927322613 35.0 34.0 35.0 27.0 35.0 114-115 31.816602580791596 35.0 34.0 35.0 26.0 35.0 116-117 31.73859972362966 35.0 34.0 35.0 25.0 35.0 118-119 31.650124500163553 35.0 34.0 35.0 25.0 35.0 120-121 31.548736417911112 35.0 34.0 35.0 25.0 35.0 122-123 31.409435309982044 35.0 34.0 35.0 25.0 35.0 124-125 30.153680376148486 34.5 31.5 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1543693570742164 1101 2 -0.07559311053427109 1101 3 0.0035599357531594933 1101 4 -0.011509590137279702 1101 5 -0.1515494084406157 1101 6 0.06102281788645314 1101 7 0.04274823913856096 1101 8 0.13011001742542305 1101 9 0.05370616975880438 1101 10-11 -0.1274363741827642 1101 12-13 -0.12115431272776078 1101 14-15 -0.07667350535515993 1101 16-17 -0.08793384835889384 1101 18-19 -0.030347621582706097 1101 20-21 -0.09085476517277158 1101 22-23 -0.017700597520985184 1101 24-25 -0.05571254834661232 1101 26-27 0.028700020200169263 1101 28-29 -0.06671830158926184 1101 30-31 0.06267655118490723 1101 32-33 0.13638168765398717 1101 34-35 0.15556584037018695 1101 36-37 0.17661184783860762 1101 38-39 0.27768118027453426 1101 40-41 0.3343358800044811 1101 42-43 0.27625982109801583 1101 44-45 0.20639929682185 1101 46-47 0.37144692020480363 1101 48-49 0.28987699513886156 1101 50-51 0.29899571324919805 1101 52-53 0.3949360183867725 1101 54-55 0.5609994256880242 1101 56-57 0.34050509198501544 1101 58-59 0.42513081649227047 1101 60-61 0.5265818092495635 1101 62-63 0.504789514994556 1101 64-65 0.5105147062238018 1101 66-67 0.5250656885661442 1101 68-69 0.5260873112575624 1101 70-71 0.4813568298264599 1101 72-73 0.40243973418702694 1101 74-75 0.35534902824461767 1101 76-77 0.4166044057108351 1101 78-79 0.3607419685882647 1101 80-81 0.39503689933565767 1101 82-83 0.395366831164246 1101 84-85 0.3922135993723401 1101 86-87 0.3112210033294147 1101 88-89 0.2812658550776206 1101 90-91 0.2192574235039615 1101 92-93 0.2948598636708937 1101 94-95 0.3588506626541488 1101 96-97 0.2547654909461343 1101 98-99 0.17459078741460843 1101 100-101 0.32129610714546786 1101 102-103 0.2265917250680083 1101 104-105 0.2936130974725657 1101 106-107 0.25050527153599944 1101 108-109 0.18070031236687356 1101 110-111 0.2454761842760469 1101 112-113 0.24596709526637994 1101 114-115 0.18103764482373919 1101 116-117 0.18855362201458448 1101 118-119 0.2420018879382937 1101 120-121 0.25851715486119886 1101 122-123 0.2611857474870902 1101 124-125 0.2853210973325879 1102 1 -0.12044957505148446 1102 2 -0.178983379685409 1102 3 -0.03311141859661859 1102 4 0.007082444707826596 1102 5 0.028914183887209788 1102 6 0.02240070374136849 1102 7 0.07851867939013601 1102 8 0.09467162139433327 1102 9 -0.01370369000134275 1102 10-11 -0.04962937976893045 1102 12-13 0.015232850833321265 1102 14-15 0.08766641026655009 1102 16-17 0.0648711928536585 1102 18-19 0.029464285132398516 1102 20-21 -0.023107978188903644 1102 22-23 -0.0608700084354723 1102 24-25 0.06459610628834156 1102 26-27 0.0237425511584064 1102 28-29 0.0421848782224572 1102 30-31 0.05506516988094745 1102 32-33 0.07913457839191551 1102 34-35 0.13233006944163606 1102 36-37 0.0052294207906768975 1102 38-39 0.08771160138472567 1102 40-41 -0.03542954051975755 1102 42-43 0.07779625806821144 1102 44-45 -0.10888645652788398 1102 46-47 -0.1015537341176298 1102 48-49 0.019099816342809106 1102 50-51 -0.061658023224879344 1102 52-53 -0.11266315540908067 1102 54-55 0.002925868127185538 1102 56-57 -0.005506959266980971 1102 58-59 -0.18097562321240446 1102 60-61 -0.24502303859605234 1102 62-63 -0.09941133269481384 1102 64-65 -0.08230996466178908 1102 66-67 0.06598525573674152 1102 68-69 0.05824921211306133 1102 70-71 0.02398422738104955 1102 72-73 -0.035905025373899946 1102 74-75 0.07317980288424764 1102 76-77 0.004941596616738764 1102 78-79 -0.0792031952853236 1102 80-81 -0.1104064285287194 1102 82-83 -0.09146126758961515 1102 84-85 -0.09617950192067326 1102 86-87 -0.12817726418103348 1102 88-89 -0.12548343200390377 1102 90-91 -0.064480466648007 1102 92-93 -0.0795822532086703 1102 94-95 -0.168330574894199 1102 96-97 -0.06456827209148486 1102 98-99 -0.14162761955441283 1102 100-101 -0.05960256077124981 1102 102-103 -0.05274779991343692 1102 104-105 -0.003523753576303079 1102 106-107 0.04360876190671803 1102 108-109 0.08593586844328627 1102 110-111 0.18872836209549249 1102 112-113 0.14379269392257754 1102 114-115 -0.032789067157192875 1102 116-117 -0.03811294699201895 1102 118-119 -0.045475161946466613 1102 120-121 -0.02061240098082351 1102 122-123 0.078396624865114 1102 124-125 0.26472214815488826 1103 1 0.25778114953088505 1103 2 0.30445530552318445 1103 3 0.337758986670174 1103 4 0.27020292749770647 1103 5 0.3473940954711452 1103 6 0.37384024766713964 1103 7 0.40185590875508126 1103 8 0.45414971217982014 1103 9 0.31125715090539785 1103 10-11 0.3068388278179981 1103 12-13 0.4569943341877192 1103 14-15 0.5514158238740379 1103 16-17 0.5512374675318341 1103 18-19 0.5889578805334921 1103 20-21 0.4097800943886014 1103 22-23 0.3446655368896856 1103 24-25 0.3397143786766037 1103 26-27 0.42973647652952707 1103 28-29 0.5219680521365291 1103 30-31 0.46156955490428686 1103 32-33 0.5337644951440481 1103 34-35 0.6175720607113391 1103 36-37 0.6233213194302465 1103 38-39 0.6221413893293004 1103 40-41 0.5789010973957858 1103 42-43 0.5414379344749563 1103 44-45 0.43459603915864875 1103 46-47 0.42388615965587206 1103 48-49 0.5188592960238125 1103 50-51 0.470225032218039 1103 52-53 0.48614497696058123 1103 54-55 0.5994917945478448 1103 56-57 0.5057561500842809 1103 58-59 0.4841270650494067 1103 60-61 0.49863676628432074 1103 62-63 0.4570644284227967 1103 64-65 0.43021378920443 1103 66-67 0.44012276179943655 1103 68-69 0.4052122952257591 1103 70-71 0.31803840607373957 1103 72-73 0.27856356386510583 1103 74-75 0.34921205248470244 1103 76-77 0.305867102902063 1103 78-79 0.3713975091807953 1103 80-81 0.3570310958214833 1103 82-83 0.29440011319579895 1103 84-85 0.3523296696557878 1103 86-87 0.38223309131197425 1103 88-89 0.3861812071361044 1103 90-91 0.3303875055868062 1103 92-93 0.2772978205774024 1103 94-95 0.35141992006084166 1103 96-97 0.4188382112231679 1103 98-99 0.5052468089613598 1103 100-101 0.6046839486229523 1103 102-103 0.4730983942997611 1103 104-105 0.4534413968184694 1103 106-107 0.4155326859261521 1103 108-109 0.47565670585155573 1103 110-111 0.6138470320379987 1103 112-113 0.5296226679225313 1103 114-115 0.4647429160819243 1103 116-117 0.5138950995059233 1103 118-119 0.5172712222976621 1103 120-121 0.5406753567337184 1103 122-123 0.6765690679949792 1103 124-125 0.5045352181482841 1104 1 -0.09426117717839944 1104 2 0.17396527308530807 1104 3 0.19500869535912102 1104 4 0.20101104011698823 1104 5 0.1618273773657748 1104 6 0.3431770032492878 1104 7 0.3549516792334657 1104 8 0.3606498853918012 1104 9 0.5071913933391841 1104 10-11 0.4507055490726799 1104 12-13 0.4498202089082213 1104 14-15 0.49610294005203315 1104 16-17 0.4779459675160922 1104 18-19 0.3539359928378403 1104 20-21 0.38270627459204576 1104 22-23 0.31830661017409767 1104 24-25 0.3246819407967152 1104 26-27 0.2849422838548179 1104 28-29 0.38945852547763593 1104 30-31 0.47391327045872345 1104 32-33 0.42391424477401074 1104 34-35 0.3819667635740629 1104 36-37 0.36028396862874246 1104 38-39 0.43963028801603343 1104 40-41 0.3280114176804858 1104 42-43 0.315030193474108 1104 44-45 0.4403592741285749 1104 46-47 0.43407102407812914 1104 48-49 0.41042859656234754 1104 50-51 0.33259079293355853 1104 52-53 0.2696733049925797 1104 54-55 0.34873989708933095 1104 56-57 0.38378027378912805 1104 58-59 0.4423877018396283 1104 60-61 0.3288827272958841 1104 62-63 0.4243218529181121 1104 64-65 0.3218752070232256 1104 66-67 0.3678760449537819 1104 68-69 0.4264850883476612 1104 70-71 0.34047959273326 1104 72-73 0.40950237279654544 1104 74-75 0.47183904835911505 1104 76-77 0.33192527063425104 1104 78-79 0.3675451172808195 1104 80-81 0.3122306045293186 1104 82-83 0.37620970599017767 1104 84-85 0.3539444777054186 1104 86-87 0.26728017347743815 1104 88-89 0.33926910434267654 1104 90-91 0.34865585818341316 1104 92-93 0.2656406543253951 1104 94-95 0.3844482953324686 1104 96-97 0.30222796967118626 1104 98-99 0.2943494134396758 1104 100-101 0.27772114195901665 1104 102-103 0.14221076163513047 1104 104-105 0.219842209340122 1104 106-107 0.1978642766419867 1104 108-109 0.26665755616490827 1104 110-111 0.25188123353077785 1104 112-113 0.1838474655794471 1104 114-115 0.22099586855885178 1104 116-117 0.4638998234690135 1104 118-119 0.394311187025405 1104 120-121 0.16728307883871096 1104 122-123 0.23332039857791287 1104 124-125 0.22185405416378146 1105 1 -0.27902002235591183 1105 2 -0.24017304326415356 1105 3 -0.09128598599586724 1105 4 -0.17399351455732415 1105 5 -0.2216853089204065 1105 6 -0.3102396097734399 1105 7 -0.2952068234325367 1105 8 -0.2202046929668171 1105 9 -0.24813757560401228 1105 10-11 -0.22425619166310895 1105 12-13 -0.19748858586422813 1105 14-15 -0.0955963178396928 1105 16-17 -0.06978853748191227 1105 18-19 -0.028233064526759222 1105 20-21 -0.10044446690893238 1105 22-23 -0.07948801652479887 1105 24-25 -0.06790062026627908 1105 26-27 -0.06835165660648101 1105 28-29 0.0024159666008785052 1105 30-31 -0.009522897299042654 1105 32-33 0.037008326126560576 1105 34-35 -0.020872924036005713 1105 36-37 0.14471882464030728 1105 38-39 0.11531318551764969 1105 40-41 0.02088061958008325 1105 42-43 0.04389194999944124 1105 44-45 -0.0648452003834663 1105 46-47 -0.12187707692632443 1105 48-49 -0.1866588410386143 1105 50-51 -0.16978674705647734 1105 52-53 -0.04098888392316269 1105 54-55 0.030734054960419144 1105 56-57 -0.046968939770437146 1105 58-59 -0.09530526452866184 1105 60-61 -0.09255369552364812 1105 62-63 -0.06629855445195432 1105 64-65 -0.039148015742867415 1105 66-67 -0.10856566128793332 1105 68-69 -0.09169413504999824 1105 70-71 -0.07323058423101259 1105 72-73 -0.17000857664049107 1105 74-75 -0.14210979353903497 1105 76-77 -0.07683397535459591 1105 78-79 -0.14881527284222074 1105 80-81 -0.1267656059816531 1105 82-83 -0.1387721360415739 1105 84-85 -0.10902298370130836 1105 86-87 -0.10202496540976114 1105 88-89 -0.0885590447591511 1105 90-91 0.015340237472400986 1105 92-93 -0.03224263670007588 1105 94-95 -0.04906408730875711 1105 96-97 -0.09226299992788256 1105 98-99 -0.040537569908437376 1105 100-101 -0.04374321619627963 1105 102-103 -0.05384367583222982 1105 104-105 -0.04019477461527998 1105 106-107 -0.11124628655867141 1105 108-109 -0.13107937799271951 1105 110-111 -0.07604468924624186 1105 112-113 -0.07570216822474407 1105 114-115 -0.18986308228710413 1105 116-117 -0.10866221119509234 1105 118-119 -0.13507557162543904 1105 120-121 -0.03923040907014297 1105 122-123 -0.12037587144746809 1105 124-125 -0.14338679701375412 1106 1 -0.011487292009189076 1106 2 0.07633321288938077 1106 3 0.08034776135255584 1106 4 0.10473183183465551 1106 5 0.03327052915156514 1106 6 0.07393328338540783 1106 7 0.21675650939368296 1106 8 0.04183327165197426 1106 9 0.09180449864314255 1106 10-11 0.0846401332951956 1106 12-13 0.02732784863058413 1106 14-15 0.18081436656254368 1106 16-17 0.21732929750719876 1106 18-19 0.1817825937974078 1106 20-21 0.14175758718763376 1106 22-23 0.2527842058090499 1106 24-25 0.263164217538737 1106 26-27 0.23260607022393742 1106 28-29 0.22052450993180628 1106 30-31 0.30203972021015346 1106 32-33 0.2695129116761308 1106 34-35 0.3533305251009864 1106 36-37 0.2919623280862922 1106 38-39 0.3818703204605356 1106 40-41 0.27885904525112437 1106 42-43 0.28063834829783474 1106 44-45 0.3177338734359907 1106 46-47 0.2688652080121159 1106 48-49 0.33666000607242097 1106 50-51 0.25566829159417637 1106 52-53 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-1.038104741210848 2216 102-103 -1.0459842735180693 2216 104-105 -0.9908474009367652 2216 106-107 -1.0496259585806484 2216 108-109 -1.0755757569155548 2216 110-111 -1.0367551695592354 2216 112-113 -1.0322292638711232 2216 114-115 -1.0955053032144306 2216 116-117 -1.0294288806365302 2216 118-119 -1.019191113122151 2216 120-121 -1.0033501127245188 2216 122-123 -1.0409444221841717 2216 124-125 -1.0387218507055884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12880.0 3 1886.0 4 1221.0 5 1038.0 6 1118.0 7 1279.0 8 1491.0 9 1628.0 10 1817.0 11 2096.0 12 2481.0 13 2533.0 14 2653.0 15 2616.0 16 2246.0 17 2228.0 18 1916.0 19 1717.0 20 1768.0 21 1657.0 22 1749.0 23 2256.0 24 3620.0 25 6241.0 26 7027.0 27 6134.0 28 6956.0 29 8128.0 30 9195.0 31 11169.0 32 14603.0 33 20028.0 34 30521.0 35 54248.0 36 136369.0 37 338748.0 38 192490.0 39 1043.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81595703942506 18.380473599373044 13.499986641612269 24.30358271958963 2 15.21351321217527 19.208161444977144 41.22466272717137 24.35366261567622 3 19.59507132380272 23.64146462521896 28.194102237090156 28.569361813888168 4 12.850279644487811 16.022665628512264 36.72292973410104 34.404124992898886 5 14.500462946476263 37.325949930558025 33.60279192336455 14.570795199601152 6 33.08293105348161 35.55960958169945 17.47053998069947 13.886919384119473 7 29.821800251549924 31.55760604610264 20.146477744509866 18.47411595783756 8 25.951525492441036 36.59878780318842 19.094661389014494 18.355025315356052 9 26.033371804412443 17.681508482204528 19.443119989661824 36.84199972372121 10-11 25.430441681726673 26.83568384500259 26.82154682387331 20.912327649397426 12-13 25.757897003804718 23.64529579922748 27.310224549090446 23.286582647877356 14-15 22.715045013917845 25.41760486231544 24.669774604928524 27.197575518838192 16-17 23.1422669535148 28.64782565628753 25.397634504580406 22.812272885617265 18-19 23.079106689918007 27.583784695416174 26.75427293458362 22.5828356800822 20-21 23.83758135759884 26.80519662952665 26.509477505278905 22.847744507595603 22-23 23.456843066709737 26.729117702752376 26.373402987402116 23.44063624313577 24-25 23.395852030669943 26.78174946942248 26.519467128690383 23.302931371217202 26-27 23.1734308720172 27.152946858183146 26.33205067145013 23.341571598349528 28-29 23.04406024541461 27.125791677979 26.596151877550838 23.23399619905555 30-31 23.33753746302378 26.7704216944219 26.526741658548637 23.36529918400569 32-33 23.076114235611367 26.992399758990963 26.42197833255352 23.509507672844148 34-35 23.466981788158687 27.087707702561808 26.227243543737018 23.218066965542487 36-37 23.624262033882466 26.77301018966258 26.29240036762006 23.3103274088349 38-39 23.201555123082386 26.867210336628066 26.422348996947836 23.508885543341712 40-41 23.49961501486532 26.745061953677162 26.441413273753362 23.313909757704153 42-43 23.322289449892203 26.63539884553863 26.438222407677863 23.6040892968913 44-45 23.196995618610476 26.827846164545516 26.525794561513315 23.44936365533069 46-47 23.277787885717593 27.08156328469838 26.210741086327182 23.429907743256845 48-49 23.381842764898497 26.884147681590438 26.340704800959248 23.39330475255182 50-51 23.34780438871892 26.923228853537452 26.412151903241437 23.31681485450219 52-53 23.54179614105582 26.7176162877066 26.313962202844106 23.426625368393466 54-55 23.290024069357138 26.769286921648288 26.460380146730717 23.480308862263854 56-57 23.231781446912684 27.073486250017385 26.43129181677818 23.26344048629175 58-59 23.379067944246025 26.70280700602531 26.47251110444662 23.44561394528205 60-61 23.317562334760584 26.796324897406908 26.628192403215323 23.257920364617178 62-63 23.086527670281328 26.99000732799656 26.544985942153897 23.37847905956821 64-65 23.043024850195437 26.891032204642386 26.49053315288524 23.57540979227694 66-67 23.03687937342626 27.103988425636103 26.28832964678714 23.570802554150493 68-69 23.076370864700984 26.825742027682185 26.57509760886115 23.52278949875568 70-71 23.14020384849306 26.785927921300708 26.5431504535871 23.530717776619134 72-73 23.11993679337666 26.873330811003292 26.44106650048963 23.56566589513042 74-75 23.073340951804454 27.02741648149034 26.387431525559897 23.511811041145307 76-77 23.168351535855166 26.92584164640558 26.31433705914753 23.59146975859172 78-79 23.222264635149287 26.911836879140594 26.480263707011005 23.385634778699117 80-81 23.13670765214314 27.121691143331205 26.414711148650525 23.326890055875126 82-83 23.308364808290246 27.003565554341318 26.48592585338494 23.202143783983495 84-85 23.341755333206844 26.945540392493754 26.361100709573428 23.351603564725973 86-87 23.21453710983907 26.89494416315965 26.522012274144448 23.368506452856835 88-89 23.254364575461775 26.863715734729972 26.41843833503424 23.463481354774014 90-91 23.230631402640906 27.118985482246494 26.46009426245472 23.19028885265788 92-93 23.29522878864944 26.71807125931977 26.57694606413183 23.409753887898958 94-95 23.19189769848296 26.868059377258824 26.58365973538758 23.35638318887063 96-97 23.295167461665333 26.83165022136057 26.485196998666716 23.387985318307376 98-99 23.238922451596245 26.767588515766562 26.419056989328695 23.57443204330851 100-101 22.994684541770553 26.74621955908349 26.725242612024964 23.53385328712099 102-103 23.141528779030136 26.600354468839292 26.703745850850417 23.55437090128015 104-105 23.03483074293269 26.750662716704465 26.62941505095852 23.585091489404324 106-107 22.956316954857865 26.873038759318934 26.531249356571156 23.639394929252042 108-109 23.154835564972757 26.760698960150993 26.436191285922867 23.648274188953387 110-111 23.028181239511603 27.016109288547224 26.582941247167852 23.372768224773317 112-113 23.059313597561417 27.00108897123102 26.565333544042126 23.37426388716544 114-115 22.966177607896157 26.917594431740415 26.56128813295277 23.55493982741066 116-117 22.859737136835616 26.956688166706634 26.694971127113586 23.488603569344164 118-119 22.849210756812045 27.07293891392391 26.456702640678714 23.621147688585328 120-121 22.94055751637733 27.10877061390013 26.57998863755871 23.370683232163834 122-123 22.871087925148114 27.152670515496958 26.4780309538541 23.49821060550083 124-125 22.998083715588674 27.22381356177402 26.31663873476264 23.461463987874662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 165.0 2 152.5 3 156.5 4 146.0 5 109.0 6 93.5 7 113.0 8 116.5 9 113.0 10 109.5 11 99.5 12 103.5 13 110.5 14 126.5 15 136.5 16 153.0 17 185.0 18 242.0 19 313.5 20 436.5 21 614.0 22 885.0 23 1290.0 24 1728.5 25 2361.0 26 3210.0 27 4142.0 28 5220.5 29 6633.0 30 8242.0 31 9800.0 32 11447.5 33 12908.0 34 14709.0 35 16489.0 36 18527.0 37 20706.0 38 22369.5 39 23763.5 40 25132.5 41 27309.5 42 29363.5 43 30589.0 44 32226.5 45 34295.5 46 35081.0 47 35234.5 48 35749.5 49 36422.0 50 36320.0 51 35458.5 52 34772.5 53 34109.0 54 33203.0 55 31609.0 56 28660.0 57 25732.5 58 23835.0 59 21315.5 60 18435.5 61 15269.0 62 11766.5 63 8926.5 64 6956.0 65 5467.5 66 4156.0 67 3408.5 68 2670.5 69 1837.0 70 1303.0 71 927.5 72 707.0 73 549.0 74 436.0 75 389.0 76 277.5 77 157.0 78 100.0 79 77.0 80 45.0 81 28.5 82 29.0 83 22.0 84 16.5 85 13.0 86 17.0 87 14.0 88 8.5 89 7.0 90 3.0 91 3.5 92 2.0 93 3.0 94 5.5 95 5.0 96 4.0 97 4.0 98 3.0 99 2.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0536274162933887 2 0.21072681838107507 3 0.02403220315222398 4 0.116489429168419 5 0.022474560355320576 6 0.041500054517497886 7 0.04049871271948856 8 0.10235938379650955 9 0.12794922974563694 10-11 0.049343898601904336 12-13 0.033878730832649084 14-15 0.014185675471798878 16-17 0.03259923853519271 18-19 0.05501816879062388 20-21 0.2033836451956733 22-23 0.11393044457350629 24-25 0.07966230304163134 26-27 0.01579894836859169 28-29 0.007788213984517031 30-31 0.008288884883521697 32-33 0.008066364483964068 34-35 0.010347198579429768 36-37 0.004450407991152589 38-39 0.009679637380756882 40-41 0.0064530915871712545 42-43 0.011849211276443769 44-45 0.011849211276443769 46-47 0.017857262064499762 48-49 0.019081124262066724 50-51 0.011515430677107323 52-53 0.014296935671577693 54-55 0.015687688168812875 56-57 0.017412221265384505 58-59 0.018636083462951467 60-61 0.010903499578323842 62-63 0.020638767058970133 64-65 0.03226545793585627 66-67 0.02853824124326598 68-69 0.03449066193143256 70-71 0.02609051684813205 72-73 0.017134070765937467 74-75 0.011237280177660288 76-77 0.012127361775890804 78-79 0.024588504151118056 80-81 0.020360616559523093 82-83 0.021806999156647685 84-85 0.01730096106560569 86-87 0.02653555764724731 88-89 0.028148830544040125 90-91 0.02659118774713672 92-93 0.033489320133423234 94-95 0.02609051684813205 96-97 0.028927651942491826 98-99 0.03449066193143256 100-101 0.020972547658306574 102-103 0.029650843241054124 104-105 0.02447724395133924 106-107 0.031987307436409235 108-109 0.022474560355320576 110-111 0.02024935635974428 112-113 0.026646817847026126 114-115 0.014352565771467098 116-117 0.01262803267489547 118-119 0.03059655493917405 120-121 0.024421613851449833 122-123 0.018469193163283243 124-125 0.020026835960186652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 898794.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.84946922784938 #Duplication Level Percentage of deduplicated Percentage of total 1 81.85060816716964 54.716697119519644 2 10.816446030629416 14.461473521585097 3 3.2492037438589185 6.516226370703294 4 1.4157433588061974 3.7856676839618806 5 0.7257603862288535 2.4258348302998916 6 0.43847387605241855 1.7587047530629198 7 0.2849335649430576 1.3333360307149644 8 0.21272834634605103 1.13766216343613 9 0.15664322406151998 0.9424364747986528 >10 0.7959490996112502 9.32786198418672 >50 0.03951017065294326 1.785928308958332 >100 0.01366669757536697 1.693189419568928 >500 3.3333406426971305E-4 0.1149813392034795 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.668902996682221E-4 0.0 28-29 0.0 0.0 0.0 5.563009988940736E-4 0.0 30-31 0.0 0.0 0.0 0.0020583136959080725 0.0 32-33 0.0 0.0 0.0 0.006341831387392439 0.0 34-35 0.0 0.0 0.0 0.011348540377439102 0.0 36-37 0.0 0.0 0.0 0.019637425260960798 0.0 38-39 0.0 0.0 0.0 0.03371184053298086 0.0 40-41 0.0 0.0 0.0 0.05173599289714885 0.0 42-43 0.0 0.0 0.0 0.06898132386286512 0.0 44-45 0.0 0.0 0.0 0.09028765212050816 0.0 46-47 0.0 0.0 0.0 0.11443111547251095 0.0 48-49 0.0 0.0 0.0 0.13946466042274425 0.0 50-51 0.0 0.0 0.0 0.17145196785915348 0.0 52-53 0.0 0.0 0.0 0.2053306986918026 0.0 54-55 0.0 0.0 0.0 0.241712784019475 0.0 56-57 0.0 0.0 0.0 0.28571619303199625 0.0 58-59 0.0 0.0 0.0 0.332389846839209 0.0 60-61 0.0 0.0 0.0 0.3838476892369108 0.0 62-63 0.0 0.0 0.0 0.4364737637322902 0.0 64-65 0.0 0.0 0.0 0.4896561392265636 0.0 66-67 0.0 0.0 0.0 0.5531857133002668 0.0 68-69 0.0 0.0 0.0 0.6298996210477597 0.0 70-71 0.0 0.0 0.0 0.7140123320805435 0.0 72-73 0.0 0.0 0.0 0.8024085608048117 0.0 74-75 0.0 0.0 0.0 0.8891915166322872 0.0 76-77 0.0 0.0 0.0 0.9897151071324464 0.0 78-79 0.0 0.0 0.0 1.1008640467114823 0.0 80-81 0.0 0.0 0.0 1.229870248355018 0.0 82-83 0.0 0.0 0.0 1.369446168977541 0.0 84-85 0.0 0.0 0.0 1.5074088167032713 0.0 86-87 0.0 0.0 0.0 1.6563862242071041 0.0 88-89 0.0 0.0 0.0 1.8257242482704603 0.0 90-91 0.0 0.0 0.0 2.014143396595883 0.0 92-93 0.0 0.0 0.0 2.218027712690561 0.0 94-95 0.0 0.0 0.0 2.4157370876975146 0.0 96-97 0.0 0.0 0.0 2.623237360285004 0.0 98-99 0.0 0.0 0.0 2.8506532086329015 0.0 100-101 0.0 0.0 0.0 3.1239082592896703 0.0 102-103 0.0 0.0 0.0 3.4134629292140355 0.0 104-105 0.0 0.0 0.0 3.7002360941439303 0.0 106-107 0.0 0.0 0.0 3.986397327975042 0.0 108-109 0.0 0.0 0.0 4.290860864669769 0.0 110-111 0.0 0.0 0.0 4.645614011664519 0.0 112-113 0.0 0.0 0.0 5.023119869514037 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGCA 1435 0.0 48.588345 5 TCAACGC 1445 0.0 47.871723 4 GTATCAA 1485 0.0 47.4463 1 ACCGTAT 65 3.487114E-5 45.841072 8 GGTATCA 815 0.0 45.423527 1 ATCAACG 1540 0.0 45.3033 3 AACGCAG 1555 0.0 44.455524 6 TATCAAC 1745 0.0 40.02134 2 GTACATG 3685 0.0 35.647736 1 TACATGG 3745 0.0 34.10854 2 ACGCAGA 2025 0.0 33.548878 7 ACATGGG 3670 0.0 32.82086 3 CGCAGAG 2130 0.0 31.615274 8 CATGGGG 2370 0.0 29.18761 4 GCAGAGT 2590 0.0 25.764368 9 CATGGGA 1860 0.0 23.40447 4 GTGTAGC 665 0.0 21.54946 1 ATGGGAG 1175 0.0 21.30147 5 GAGTACA 2645 0.0 20.542933 1 GTCTAGG 350 1.4679244E-9 20.471987 1 >>END_MODULE