FastQCFastQC Report
Fri 27 May 2016
SRR940013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898794
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.2548971176932645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.18691713562840873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.11660068936819783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.075.875241
GTATCAA26300.055.7932781
ATCAACG32350.045.1569673
TCAACGC32650.044.742054
CAACGCA33000.044.383855
AACGCAG34350.042.6134036
TATCAAC34850.042.0910222
ACGCAGA41350.035.535527
CGCAGAG42100.034.9024668
GTGGTAT7450.032.8269161
TATCACG1102.0093024E-532.5248832
GCAGAGT46750.031.049139
TGGTATC7150.030.0229662
GTACATG36900.029.581981
TACATGG37200.028.6924272
ACATGGG36700.028.4318393
CAGAGTA45500.026.14928410-11
GAGTACT28250.025.69109312-13
AGAGTAC43850.025.57307610-11
GTACTTT28900.024.70157414-15
Produced by FastQC (version 0.11.5)