FastQCFastQC Report
Fri 27 May 2016
SRR940012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170321
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.059.1600041
GTATCAA20100.058.7708931
ATCAACG25250.045.801523
TCAACGC25150.045.5154234
CAACGCA26350.043.4072845
TATCAAC27850.041.5558782
AACGCAG28300.040.6268276
ACGCAGA36150.031.4750967
GTACATG57950.031.1948091
CGCAGAG36850.030.3922148
TACATGG59800.029.5288432
TATTCCG1653.3719698E-728.8833835
ACATGGG62300.027.2707043
GCAGAGT42800.025.4592089
TAAGGTG5000.023.828795
GTGGTAT8100.023.569921
TGTACGC1300.002021127122.944712
GTATAGG3153.9835868E-1022.7281381
GTAAGGT4500.022.5232874
CATGGGG36650.021.6358054
Produced by FastQC (version 0.11.5)