FastQCFastQC Report
Fri 27 May 2016
SRR940012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170321
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45010.3845953375185099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.26796067061942835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.16995337176723307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.070.302671
GTATCAA40700.059.9222071
GTGGTAT7250.048.525831
ATCAACG52700.045.4739653
TATCAAC53750.044.6984562
TCAACGC54150.044.2562834
CAACGCA54750.043.703935
AACGCAG57300.041.7411466
ACGCAGA67950.035.022327
CGCAGAG68850.034.3916938
GTACATG59550.033.1440161
TACATGG60950.032.0823442
TGGTATC11150.031.0112362
GCAGAGT76400.030.9151619
TATCACG1352.2953245E-630.9122242
ACATGGG61800.030.5786363
GGACCGA3450.025.8681346
AGTACTT47900.025.71042312-13
GTATAGG3250.025.686421
AGAGTAC74450.025.65161710-11
Produced by FastQC (version 0.11.5)