FastQCFastQC Report
Fri 27 May 2016
SRR940011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519058
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45460.8758173460384002No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.21635347109571568No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.13832750867918422No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.12291497289320269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.047.5073431
GGTATCA9150.039.7764851
ATCAACG17950.038.8638043
TCAACGC17950.038.2105374
CAACGCA18400.037.2400555
TATTCCG650.001924528236.667135
AACGCAG18850.036.3510326
TATCAAC20200.036.330512
GTACATG33450.033.355171
CGCAGAG21250.031.6847158
TACATGG33900.031.5043872
ACGCAGA21350.031.2572297
TAGGGCG800.005297563529.8208374
ACATGGG36150.028.039133
GTATAGG2404.2018655E-1027.3463341
GCGTCGT557.7829184E-427.02627426-27
CGATCGG450.00954584526.43460580-81
GAGTACA25550.026.1544021
GCAGAGT24150.026.1478029
AGTACAT24650.025.6570452
Produced by FastQC (version 0.11.5)