##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519058 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89753360896085 34.0 31.0 34.0 26.0 34.0 2 30.925967810919012 34.0 31.0 34.0 26.0 34.0 3 31.067445256599456 34.0 31.0 34.0 26.0 34.0 4 33.97259458480555 37.0 35.0 37.0 28.0 37.0 5 34.301827541430825 37.0 35.0 37.0 31.0 37.0 6 34.11367515768951 37.0 36.0 37.0 30.0 37.0 7 34.1602980784421 37.0 35.0 37.0 30.0 37.0 8 34.042625294283106 37.0 35.0 37.0 30.0 37.0 9 35.530482142650726 39.0 38.0 39.0 27.0 39.0 10-11 35.644014734384214 39.0 38.0 39.0 29.5 39.0 12-13 35.5983868854733 39.0 38.0 39.0 29.0 39.0 14-15 37.04971506074466 41.0 39.0 41.0 29.0 41.0 16-17 37.16064293393031 41.0 39.0 41.0 30.0 41.0 18-19 37.16539962778726 41.0 39.0 41.0 30.0 41.0 20-21 37.151281745007296 41.0 39.0 41.0 30.0 41.0 22-23 37.22643134293278 41.0 39.0 41.0 29.5 41.0 24-25 37.36110704391417 41.0 39.0 41.0 30.0 41.0 26-27 37.435534179224675 41.0 39.0 41.0 30.0 41.0 28-29 37.392064085323796 41.0 39.0 41.0 30.0 41.0 30-31 37.33743435223039 41.0 39.0 41.0 30.0 41.0 32-33 37.28069791815173 41.0 38.0 41.0 30.0 41.0 34-35 37.19549067734242 41.0 38.0 41.0 30.0 41.0 36-37 37.06748667778938 41.0 38.0 41.0 30.0 41.0 38-39 36.96363797494692 41.0 38.0 41.0 29.0 41.0 40-41 36.85907740560785 41.0 38.0 41.0 29.0 41.0 42-43 36.70799120714834 40.5 38.0 41.0 28.0 41.0 44-45 36.59861807351009 40.0 37.5 41.0 28.0 41.0 46-47 36.4533337314905 40.0 37.0 41.0 28.0 41.0 48-49 36.3174635589857 40.0 37.0 41.0 28.0 41.0 50-51 36.178830689441256 40.0 36.5 41.0 28.0 41.0 52-53 36.288799132274235 40.0 36.5 41.0 29.0 41.0 54-55 36.10789353020279 40.0 36.0 41.0 28.5 41.0 56-57 35.99728932026864 40.0 35.5 41.0 28.5 41.0 58-59 35.81528942815639 40.0 35.0 41.0 28.0 41.0 60-61 35.57477488064917 39.5 35.0 41.0 28.0 41.0 62-63 35.29078734939063 39.0 35.0 41.0 28.0 41.0 64-65 34.978747076434615 39.0 35.0 41.0 27.0 41.0 66-67 34.65154664796613 37.5 35.0 41.0 26.5 41.0 68-69 34.28942911967449 37.0 35.0 40.0 26.0 41.0 70-71 33.90856513144966 37.0 35.0 39.5 25.5 41.0 72-73 33.52708945821084 36.0 34.0 39.0 25.0 41.0 74-75 33.14781199788848 36.0 34.0 39.0 25.0 41.0 76-77 32.77963348989901 35.0 34.0 37.5 24.0 39.5 78-79 32.43141903216981 35.0 34.0 37.0 23.5 39.0 80-81 32.111926798161285 35.0 34.0 36.5 22.5 39.0 82-83 31.8035575985728 35.0 33.5 36.0 21.0 38.0 84-85 31.554375426252943 35.0 33.0 36.0 20.0 37.0 86-87 31.337338023881724 35.0 33.0 35.5 20.0 37.0 88-89 31.15771936854841 35.0 33.0 35.0 19.0 36.5 90-91 30.99582801921943 35.0 33.0 35.0 18.0 36.0 92-93 30.838813774183233 35.0 33.0 35.0 16.0 36.0 94-95 30.712297084333542 35.0 33.0 35.0 13.5 36.0 96-97 30.603820767621343 35.0 33.0 35.0 10.0 35.5 98-99 30.524422126236374 35.0 33.0 35.0 7.5 35.0 100-101 30.418902126544626 35.0 32.0 35.0 7.0 35.0 102-103 29.786362988336563 34.0 31.0 35.0 7.0 35.0 104-105 30.584141849273106 35.0 33.0 35.0 7.0 35.0 106-107 30.671781380886145 35.0 33.0 35.0 3.5 35.0 108-109 30.645044484431413 35.0 33.0 35.0 2.0 35.0 110-111 30.586071306096812 35.0 33.0 35.0 2.0 35.0 112-113 30.52693725941995 35.0 33.0 35.0 2.0 35.0 114-115 30.455884506163088 35.0 33.0 35.0 2.0 35.0 116-117 30.36576548285548 35.0 33.0 35.0 2.0 35.0 118-119 30.2726487213375 35.0 33.0 35.0 2.0 35.0 120-121 30.161642822189428 35.0 33.0 35.0 2.0 35.0 122-123 30.01587009544213 35.0 33.0 35.0 2.0 35.0 124-125 28.871250419028314 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21678959484785665 1101 2 0.46779984195790547 1101 3 0.49160845504169615 1101 4 0.41145433481542426 1101 5 0.49302408150699506 1101 6 0.34556157511720187 1101 7 0.31990912874495336 1101 8 0.4065327748017893 1101 9 0.9035878108099524 1101 10-11 0.4667882811189443 1101 12-13 0.7246566810733057 1101 14-15 0.8791794705145364 1101 16-17 0.7135698550210705 1101 18-19 0.46348615598567733 1101 20-21 0.4154083262852666 1101 22-23 0.49626698512465595 1101 24-25 0.3273405826268032 1101 26-27 0.4382659704146121 1101 28-29 0.3742858409981622 1101 30-31 0.39520433199795946 1101 32-33 0.4696382644084096 1101 34-35 0.4874072283446935 1101 36-37 0.5449439059078287 1101 38-39 0.5524859432674134 1101 40-41 0.6135741819553857 1101 42-43 0.552258399973482 1101 44-45 0.7184137927078282 1101 46-47 0.7290822575351186 1101 48-49 0.6742458038778096 1101 50-51 0.6209807634133142 1101 52-53 0.685735116109619 1101 54-55 0.6766159787644881 1101 56-57 0.5764930031713789 1101 58-59 0.6198107815793961 1101 60-61 0.4459487158190356 1101 62-63 0.3769546557985919 1101 64-65 0.5224504817104787 1101 66-67 0.30787994295830856 1101 68-69 0.2452985572126707 1101 70-71 0.45980224031921324 1101 72-73 0.2755960505457935 1101 74-75 0.3965475048020366 1101 76-77 0.29905333430370007 1101 78-79 0.46476628797825725 1101 80-81 0.4029879847785338 1101 82-83 0.42408784063087523 1101 84-85 0.3811274591361311 1101 86-87 0.41971833140972237 1101 88-89 0.44093831509939463 1101 90-91 0.46754466939128747 1101 92-93 0.4415537285581479 1101 94-95 0.4590971769261749 1101 96-97 0.5806572677424882 1101 98-99 0.5039357013180386 1101 100-101 0.4898186990023987 1101 102-103 0.4563494246266835 1101 104-105 0.44917878881982887 1101 106-107 0.5286659135623459 1101 108-109 0.6089147096176255 1101 110-111 0.6178017724799538 1101 112-113 0.7066448327366572 1101 114-115 0.6165880882510919 1101 116-117 0.6807876474295078 1101 118-119 0.7117851087297566 1101 120-121 0.4931878534084433 1101 122-123 0.40858049748921843 1101 124-125 0.5642120320061359 1102 1 -0.12645487997556515 1102 2 0.004148288832666935 1102 3 0.009630783590338865 1102 4 0.09379655399974496 1102 5 -0.04253451194633584 1102 6 0.1973957058986997 1102 7 0.15582467518681398 1102 8 0.05687833567366596 1102 9 -0.020624234454722057 1102 10-11 0.0019595426059950682 1102 12-13 0.01360632791769234 1102 14-15 -0.056174064838991455 1102 16-17 0.06902288152673464 1102 18-19 0.17795794151008693 1102 20-21 0.015165294463287182 1102 22-23 0.057188227624848764 1102 24-25 0.07978609033546036 1102 26-27 0.16752472335757318 1102 28-29 0.15483190312317419 1102 30-31 0.19099684357994562 1102 32-33 0.36135619560002397 1102 34-35 0.28342758225977605 1102 36-37 0.2968767683669995 1102 38-39 0.14611775005711536 1102 40-41 0.09229750541555148 1102 42-43 0.1280919231754254 1102 44-45 0.03913985027598699 1102 46-47 0.189308580919338 1102 48-49 0.02663435100319589 1102 50-51 -0.17162955024962656 1102 52-53 -0.23793010755560573 1102 54-55 -0.2032807327111641 1102 56-57 -0.12852712290138157 1102 58-59 -0.15909481574725248 1102 60-61 -0.07787220010714435 1102 62-63 -0.010305160408599079 1102 64-65 -0.023573669576833822 1102 66-67 0.01833790604784724 1102 68-69 -0.12788060280859526 1102 70-71 -0.003875894411550007 1102 72-73 -0.04732488816461711 1102 74-75 0.07006083357852333 1102 76-77 -0.1331445847363213 1102 78-79 -0.05777111613809183 1102 80-81 -0.005818121395989806 1102 82-83 0.043602536461360586 1102 84-85 0.05210794064293012 1102 86-87 0.06294381937205173 1102 88-89 0.11832116616645649 1102 90-91 0.07411412332916711 1102 92-93 0.01955934866903064 1102 94-95 0.06148192667934893 1102 96-97 0.07268278608379575 1102 98-99 0.044286578511020025 1102 100-101 -0.02005826613772399 1102 102-103 0.025036293313551283 1102 104-105 -0.12078399693243114 1102 106-107 -0.04834859239952749 1102 108-109 0.020489100139386096 1102 110-111 -0.08387286866836874 1102 112-113 -0.07453539054882796 1102 114-115 0.04330639917992585 1102 116-117 -0.017006079311588707 1102 118-119 -0.07054571833791101 1102 120-121 -0.0773255513154858 1102 122-123 -0.04731682156599604 1102 124-125 -0.16551000034747432 1103 1 0.12616195623260396 1103 2 0.1551532522215311 1103 3 0.24482170206299259 1103 4 0.3199354306030173 1103 5 0.048423274677084294 1103 6 0.24104895667269943 1103 7 0.2708426121861791 1103 8 0.0766237777101324 1103 9 0.043000513690152786 1103 10-11 0.23123616553586857 1103 12-13 0.28411347814620314 1103 14-15 0.4224293063340312 1103 16-17 0.4492626967510702 1103 18-19 0.48295327297601176 1103 20-21 0.31251405853031855 1103 22-23 0.41047094422025054 1103 24-25 0.4892460146560751 1103 26-27 0.48353288100659597 1103 28-29 0.7314253475184671 1103 30-31 0.6179269138536725 1103 32-33 0.6019208425114186 1103 34-35 0.7068931088212764 1103 36-37 0.6900091090225686 1103 38-39 0.5639785785990625 1103 40-41 0.5309210249988254 1103 42-43 0.6674427512259413 1103 44-45 0.7308446023522635 1103 46-47 0.5990043890671615 1103 48-49 0.5272338890640356 1103 50-51 0.47570447107819547 1103 52-53 0.47919604114949266 1103 54-55 0.5569752995262931 1103 56-57 0.4100374257250934 1103 58-59 0.3409150127455476 1103 60-61 0.33364925057839656 1103 62-63 0.4427441294827972 1103 64-65 0.3963363997140448 1103 66-67 0.3188096737022832 1103 68-69 0.3526491529533544 1103 70-71 0.4124713462390943 1103 72-73 0.24548065510532524 1103 74-75 0.01623509275587054 1103 76-77 0.1492034421937447 1103 78-79 0.1480620943390818 1103 80-81 0.10994923817941071 1103 82-83 0.02397995058490565 1103 84-85 0.230011031703917 1103 86-87 0.28989846087777593 1103 88-89 0.19391701854249632 1103 90-91 0.14073164753182965 1103 92-93 0.09724375576519861 1103 94-95 0.021556131799993494 1103 96-97 0.15446813180103192 1103 98-99 0.10687687778862554 1103 100-101 0.19687376983456062 1103 102-103 0.2201171326668394 1103 104-105 0.31607530819312757 1103 106-107 0.31691997116625004 1103 108-109 0.39376788533767737 1103 110-111 0.35616841100139496 1103 112-113 0.4689114618763526 1103 114-115 0.3038007723678007 1103 116-117 0.3755291804063354 1103 118-119 0.4390812903603063 1103 120-121 0.3682933980186327 1103 122-123 0.46013317597688186 1103 124-125 0.5823891593163886 1104 1 -0.6010678376393699 1104 2 -0.5800740801354642 1104 3 -0.5639696272982064 1104 4 -0.7121906791061292 1104 5 -0.6250252791828643 1104 6 -0.4908939705265638 1104 7 -0.601616565305271 1104 8 -0.774366314215051 1104 9 -0.6437049202472735 1104 10-11 -0.684997191808371 1104 12-13 -0.9094768036400112 1104 14-15 -0.9133218135121695 1104 16-17 -0.882434794507212 1104 18-19 -0.8174183394594081 1104 20-21 -0.863671499627749 1104 22-23 -0.7880841083254495 1104 24-25 -0.7140591416453574 1104 26-27 -0.6908936003149933 1104 28-29 -0.7205429103872376 1104 30-31 -0.6183117386099894 1104 32-33 -0.5962525798053662 1104 34-35 -0.46208321579339184 1104 36-37 -0.5599408020098906 1104 38-39 -0.5485737166682512 1104 40-41 -0.4354215739984397 1104 42-43 -0.4651773311107732 1104 44-45 -0.38853887401290876 1104 46-47 -0.4320698523235009 1104 48-49 -0.5283195463235799 1104 50-51 -0.6258497180291229 1104 52-53 -0.5720974468076889 1104 54-55 -0.597070934975946 1104 56-57 -0.7242665999967883 1104 58-59 -0.7744529980545849 1104 60-61 -0.8199259091100046 1104 62-63 -0.8040525617831165 1104 64-65 -0.7255694139454505 1104 66-67 -0.8290243362296508 1104 68-69 -0.8367575074482119 1104 70-71 -0.8579065153913277 1104 72-73 -0.8012833590722366 1104 74-75 -0.7542512949708922 1104 76-77 -0.7634855243993002 1104 78-79 -0.6974671075290004 1104 80-81 -0.8132691449487126 1104 82-83 -0.9012980431849797 1104 84-85 -0.9122523694800933 1104 86-87 -1.0234736238250512 1104 88-89 -0.9409177117164873 1104 90-91 -0.9923666651855214 1104 92-93 -0.9787555191373656 1104 94-95 -1.040362180249506 1104 96-97 -0.9684060685643772 1104 98-99 -0.9692213824545668 1104 100-101 -0.9315448022005306 1104 102-103 -1.0749808270425483 1104 104-105 -0.9376646680503917 1104 106-107 -0.9556284123912135 1104 108-109 -0.8705814112701127 1104 110-111 -0.8129689138661114 1104 112-113 -0.7135698024645976 1104 114-115 -0.789002158552357 1104 116-117 -0.7865575259816815 1104 118-119 -0.6126141019317188 1104 120-121 -0.6394858956792255 1104 122-123 -0.991130258599771 1104 124-125 -0.9673479928634379 1105 1 0.4521919633771603 1105 2 0.6002195378450921 1105 3 0.5658902104380026 1105 4 0.674662746630645 1105 5 0.2383891395655482 1105 6 0.657576701373813 1105 7 0.3357787080820245 1105 8 0.5024791400980035 1105 9 0.8627988783801541 1105 10-11 0.7803550033543942 1105 12-13 0.7562695470515024 1105 14-15 0.9210003946884058 1105 16-17 0.7847989355380776 1105 18-19 0.7567089290846098 1105 20-21 0.5672374053589238 1105 22-23 0.6743432947799164 1105 24-25 0.5694039887768909 1105 26-27 0.6331319919060192 1105 28-29 0.5619603955457819 1105 30-31 0.61570416240491 1105 32-33 0.7334204058995297 1105 34-35 0.7188007058681976 1105 36-37 0.7080094265584833 1105 38-39 0.8667490795119761 1105 40-41 0.7570859849099136 1105 42-43 0.6884596600137556 1105 44-45 0.8393625034398156 1105 46-47 0.7823416915995125 1105 48-49 0.755026220571537 1105 50-51 0.6931768851450428 1105 52-53 0.7952236667746391 1105 54-55 0.8832236126167103 1105 56-57 0.8646496349812622 1105 58-59 0.8256534425304167 1105 60-61 0.9367690410046876 1105 62-63 0.9520915931646314 1105 64-65 0.895840249837093 1105 66-67 0.9029963848239788 1105 68-69 0.8927579090063844 1105 70-71 0.7736684566622145 1105 72-73 0.7729078203033879 1105 74-75 0.7408322190557897 1105 76-77 0.6279880734551568 1105 78-79 0.7221075019758203 1105 80-81 0.6600405297567704 1105 82-83 0.7408768307944769 1105 84-85 0.7432880024112265 1105 86-87 0.7836469724281478 1105 88-89 0.7750474464221497 1105 90-91 0.7302370853344797 1105 92-93 0.8174549171528369 1105 94-95 0.8120816537496616 1105 96-97 0.6156692114231639 1105 98-99 0.6563529435185593 1105 100-101 0.8063045866957168 1105 102-103 0.581949314932455 1105 104-105 0.8610661790353902 1105 106-107 0.9762833116441385 1105 108-109 0.8570329484479409 1105 110-111 0.870199529521674 1105 112-113 0.8586471132219096 1105 114-115 0.74930366988308 1105 116-117 0.7127324827799164 1105 118-119 0.5188423445795642 1105 120-121 0.5285101162848882 1105 122-123 0.5585296195745642 1105 124-125 0.5156470778684223 1106 1 -0.10572658865903861 1106 2 0.09362074448159419 1106 3 0.06502294045308332 1106 4 -0.05305218549604973 1106 5 0.04826847591547789 1106 6 -0.0055454705718887 1106 7 0.04011505800661297 1106 8 0.02910055789588739 1106 9 -0.06427502810551289 1106 10-11 0.1299590757525877 1106 12-13 0.07393545957035741 1106 14-15 0.10126057568388092 1106 16-17 -0.06702987290837115 1106 18-19 -0.1383702564357563 1106 20-21 -0.43930482691859396 1106 22-23 -0.22203679571782686 1106 24-25 -0.28165181816126506 1106 26-27 -0.10269002921012316 1106 28-29 -0.0887371911812167 1106 30-31 -0.03934862794199745 1106 32-33 -0.04459618535235421 1106 34-35 -0.1360635475254668 1106 36-37 -0.14474321295885773 1106 38-39 -0.17503070932663434 1106 40-41 -0.2545278914098503 1106 42-43 -0.26804486487312573 1106 44-45 -0.20092784346817893 1106 46-47 -0.21334081217123213 1106 48-49 -0.23077694684927508 1106 50-51 -0.184783145020873 1106 52-53 -0.19646185358735124 1106 54-55 -0.13478166641797884 1106 56-57 -0.18949817799008173 1106 58-59 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12-13 -0.5799376666355514 2216 14-15 -0.7696214062089624 2216 16-17 -0.8486116519614555 2216 18-19 -0.849555508501254 2216 20-21 -0.7760825194926824 2216 22-23 -0.8103561941247932 2216 24-25 -0.9317408697617253 2216 26-27 -0.9796330856589393 2216 28-29 -0.9235446170952315 2216 30-31 -1.1652675909855859 2216 32-33 -1.1585785234515953 2216 34-35 -1.335142110826311 2216 36-37 -1.0478934133249567 2216 38-39 -1.0616779165695078 2216 40-41 -1.0227364700473558 2216 42-43 -1.0682582176658855 2216 44-45 -0.9935940731223596 2216 46-47 -1.0021394813445355 2216 48-49 -1.1294578700175322 2216 50-51 -0.9923098605010097 2216 52-53 -1.1031376315520234 2216 54-55 -1.0184301846982962 2216 56-57 -1.0681455831253928 2216 58-59 -1.1903422292066068 2216 60-61 -1.019017219931726 2216 62-63 -1.0841360440376278 2216 64-65 -0.953666985288308 2216 66-67 -1.0372990838195832 2216 68-69 -0.9231380553831272 2216 70-71 -1.0269878866152098 2216 72-73 -1.009205620272425 2216 74-75 -0.8708679180735288 2216 76-77 -0.8227563700167408 2216 78-79 -0.6790782035931997 2216 80-81 -0.6915984890518878 2216 82-83 -0.80443102515504 2216 84-85 -0.6875999638252779 2216 86-87 -0.7042187956038539 2216 88-89 -0.6785816600046637 2216 90-91 -0.5265733958612984 2216 92-93 -0.6896013439231155 2216 94-95 -0.6592531181095218 2216 96-97 -0.7382227367645342 2216 98-99 -0.7473590620833157 2216 100-101 -0.8365166745641694 2216 102-103 -0.9572270465612256 2216 104-105 -0.8328576349101908 2216 106-107 -0.7175510701385903 2216 108-109 -0.8664066505915216 2216 110-111 -0.7669538891337453 2216 112-113 -0.8102236439830861 2216 114-115 -0.7668194902563066 2216 116-117 -0.7511851279986281 2216 118-119 -0.7502425423997465 2216 120-121 -0.8224768722868241 2216 122-123 -0.9460605971810914 2216 124-125 -0.794880419667436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12955.0 3 2810.0 4 2625.0 5 1700.0 6 1685.0 7 1599.0 8 1454.0 9 1554.0 10 1714.0 11 2101.0 12 2411.0 13 2386.0 14 2328.0 15 2132.0 16 1758.0 17 1604.0 18 1314.0 19 1217.0 20 1017.0 21 956.0 22 1124.0 23 1839.0 24 4647.0 25 5751.0 26 6244.0 27 4122.0 28 4082.0 29 4592.0 30 4807.0 31 5956.0 32 7638.0 33 10436.0 34 15942.0 35 28877.0 36 67532.0 37 182171.0 38 115152.0 39 826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34397949152869 19.88936219425223 12.856537075229854 22.910121238989227 2 14.479915821177924 22.04384635434264 40.357953064514575 23.11828475996486 3 18.932949977453433 28.426403764698627 27.05337562581177 25.58727063203617 4 11.973614419488298 18.81245599992285 35.79778770023049 33.41614188035836 5 12.981947677555613 41.33245718161777 31.991999013365813 13.693596127460806 6 30.476254722072316 38.47583069925218 17.205882352941178 13.84203222573433 7 27.595318260748904 35.9344494832009 19.335976271375902 17.134255984674297 8 24.970301917647603 39.20243292861661 18.518875638315926 17.30838951541986 9 25.44401983838392 21.09574084326635 18.636969534461574 34.82326978388816 10-11 23.721164215438257 31.089016164519013 25.301187402603787 19.888632217438946 12-13 24.29398455958589 28.288025777705833 25.91646142401782 21.501528238690458 14-15 21.57374661249876 29.15462034456415 24.111590769619088 25.160042273318002 16-17 21.582376117608177 32.26564555091013 24.632522626777885 21.51945570470381 18-19 21.6973385173805 30.87171410892282 26.460326425339893 20.97062094835679 20-21 22.877901842373728 29.74445738817055 26.245404682325894 21.13223608712983 22-23 22.406327690589663 29.849676845721074 26.021007439009576 21.72298802467969 24-25 22.04634689704835 29.625652262349 26.419408666883243 21.908592173719406 26-27 21.7259192320513 30.02641654126356 26.28715608881045 21.960508137874694 28-29 21.842881762982643 30.045113042707595 26.28530748252267 21.826697711787094 30-31 22.06402985606625 29.822927092896563 26.235998948024513 21.87704410301268 32-33 21.69984721470284 29.97780470840743 26.256914353533233 22.0654337233565 34-35 22.09008165420082 29.989518667946008 26.153909883664923 21.766489794188256 36-37 22.149994653575796 29.76754132882243 26.051439344094902 22.03102467350688 38-39 21.898996477024554 29.874966643963692 26.321741970226665 21.904294908785086 40-41 22.19445678047822 29.59440726508365 26.376869952729177 21.834266001708954 42-43 21.75353076048631 29.69326217221248 26.33523438854743 22.21797267875378 44-45 21.954523165671667 29.853384751590283 26.317624946653396 21.874467136084654 46-47 22.081495569133654 29.894715139323242 26.002894177539098 22.020895114004006 48-49 21.907139271823755 29.801351300576222 26.257005307510962 22.03450412008906 50-51 22.001021174929193 29.887863432303806 26.31163176046704 21.799483632299957 52-53 22.032005811180934 29.75195544504378 26.190583998635837 22.02545474513945 54-55 21.945963130843598 29.755634661053282 26.29338291610268 22.005019292000444 56-57 21.861969833599236 30.082294773674924 26.2350959945124 21.820639398213444 58-59 22.001223548000173 29.741946982798073 26.144908547012662 22.11192092218909 60-61 21.93249745914155 29.83049704489733 26.417606342753373 21.819399153207744 62-63 21.779360390617626 30.022756482514506 26.298959676129584 21.898923450738287 64-65 21.717341555657264 29.933852046346924 26.25972586900489 22.089080528990916 66-67 21.71252054704768 30.23475182635071 26.034000666755308 22.018726959846298 68-69 21.9763288408301 29.92359821661722 26.254982882107686 21.845090060444996 70-71 21.707268193607813 29.91525129782289 26.29743749831389 22.0800430102554 72-73 21.703048438592873 30.1151088562912 26.10306231177274 22.078780393343187 74-75 21.790955341238714 30.29978988985836 25.921580074506736 21.987674694396194 76-77 21.775013968902332 30.086510857208914 26.101327527407946 22.0371476464808 78-79 21.86326642495763 30.072059867789076 26.216437820302207 21.848235886951084 80-81 21.803325439477117 30.246989704587364 26.062057656623033 21.887627199312483 82-83 21.98053437026703 30.32424089439385 25.989178481827324 21.70604625351179 84-85 22.055197967577193 30.208203874281892 25.888245648008184 21.848352510132727 86-87 21.960086059796723 30.0893260608373 26.08685179350659 21.86373608585939 88-89 21.96664511609847 30.008604168734205 26.106570289699377 21.918180425467952 90-91 21.92841059417607 30.246625127301424 26.038861498943533 21.78610277957897 92-93 21.964361595007965 29.947302645081532 26.236614881486357 21.851720878424146 94-95 21.745460221354858 30.091503746575953 26.237690062733588 21.9253459693356 96-97 22.002125492712526 29.992667724595496 26.081028869509588 21.92417791318239 98-99 21.76342092559171 30.017797246292883 25.995638855618758 22.223142972496653 100-101 21.55469507971177 30.213300164655127 26.195387536888433 22.03661721874467 102-103 21.709960881043685 30.14992388183377 26.22694775788642 21.91316747923612 104-105 21.50034444717433 30.36810081944705 26.10427837112617 22.027276362252444 106-107 21.393808059678197 30.512808900853987 26.00389465357143 22.089488385896384 108-109 21.475650703573994 30.62572561336911 25.84026630311731 22.05835737993959 110-111 21.276503498794234 30.86877811488069 26.063825174939716 21.790893211385367 112-113 21.331704360745483 30.96255514884769 26.02013926559415 21.68560122481267 114-115 21.167594896709723 31.05490670974964 25.787261427537842 21.99023696600279 116-117 21.13574576065448 31.021065226480566 26.051960763534982 21.79122824932997 118-119 21.148057504046864 31.241906266861946 25.6791991058352 21.930837123255994 120-121 21.120962085417645 31.296391216211006 25.807968305598163 21.774678392773183 122-123 21.225693885789322 31.148051127260324 25.86720670084271 21.759048286107642 124-125 21.34919036823866 31.242045549795222 25.636524619414786 21.77223946255134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4053.0 1 3673.5 2 3118.0 3 2374.0 4 1371.0 5 769.5 6 548.0 7 565.0 8 686.5 9 633.5 10 441.0 11 312.0 12 281.0 13 294.0 14 323.0 15 355.0 16 358.5 17 371.5 18 408.5 19 509.5 20 656.0 21 830.5 22 1062.5 23 1396.5 24 1828.5 25 2309.5 26 2886.5 27 3563.0 28 4350.5 29 5245.5 30 6165.0 31 7203.0 32 8399.5 33 9194.0 34 10027.5 35 11187.5 36 12433.0 37 13497.5 38 14053.0 39 14472.0 40 14890.0 41 15602.5 42 16395.5 43 16892.0 44 17399.5 45 18246.0 46 18726.5 47 18880.0 48 19111.0 49 19036.5 50 19057.0 51 18756.5 52 17829.5 53 17381.5 54 17065.5 55 15897.5 56 14267.5 57 12787.5 58 11520.5 59 10070.0 60 8510.5 61 7130.0 62 5792.5 63 4359.5 64 3292.0 65 2600.5 66 1916.5 67 1497.5 68 1116.5 69 715.0 70 510.0 71 371.0 72 274.0 73 242.5 74 203.5 75 130.5 76 97.0 77 79.5 78 53.5 79 33.0 80 23.5 81 20.0 82 15.0 83 10.0 84 7.5 85 6.0 86 5.5 87 5.0 88 6.0 89 6.0 90 4.0 91 2.5 92 1.5 93 1.5 94 2.5 95 2.0 96 2.0 97 3.5 98 3.5 99 3.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04777886093654274 2 0.214426904122468 3 0.025430684046869524 4 0.11424542151358807 5 0.023504117073621832 6 0.041999160016799666 7 0.0367974291890309 8 0.09748428884633316 9 0.12888733051027054 10-11 0.04556330891730789 12-13 0.030825071571963056 14-15 0.010499790004199917 16-17 0.028609519552728214 18-19 0.05529247213220873 20-21 0.1933309957654058 22-23 0.11077760096174223 24-25 0.07369118672672417 26-27 0.012522685326109992 28-29 0.005490715873755919 30-31 0.006646656057704534 32-33 0.005587044222418304 34-35 0.007706267892990764 36-37 0.0037568055978329973 38-39 0.007031969452354072 40-41 0.00529805917643115 42-43 0.009247521471588917 44-45 0.008958536425601763 46-47 0.016375819272605376 48-49 0.014738237345344836 50-51 0.009247521471588917 52-53 0.011270416793498992 54-55 0.011655730188148531 56-57 0.013485968812733838 58-59 0.014545580648020067 60-61 0.007802596241653149 62-63 0.016375819272605376 64-65 0.028031549460753907 66-67 0.024467400560245676 68-69 0.030150773131326365 70-71 0.02157755010037414 72-73 0.013678625510058606 74-75 0.008573223030952225 76-77 0.009247521471588917 78-79 0.02205919184368606 80-81 0.01714644606190445 82-83 0.017724416153878757 84-85 0.01377495385872099 86-87 0.022444505238335598 88-89 0.023793102119608986 90-91 0.021673878449036524 92-93 0.029187489644702518 94-95 0.022637161935660368 96-97 0.02302247533030991 98-99 0.030343429828651132 100-101 0.01820605789719068 102-103 0.024660057257570443 104-105 0.021288565054386987 106-107 0.02726092267145483 108-109 0.019361998081139295 110-111 0.01801340119986591 112-113 0.023407788724959445 114-115 0.01146307349082376 116-117 0.009825491563563224 118-119 0.026586624230818135 120-121 0.01965098312712645 122-123 0.015123550739994376 124-125 0.016857461015917296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 519058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.4877955733448 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47328580353471 55.11437947227835 2 12.018019314678051 16.461753000403593 3 3.6694094189700897 7.539292864839882 4 1.5527369217349534 4.2537411549987265 5 0.7538234493008303 2.581385314705446 6 0.42302375832748257 1.7383178809800386 7 0.28098613481018525 1.347088467187699 8 0.1804682956580396 0.9887900592398212 9 0.118902911290436 0.7329058453381438 >10 0.498595335227464 5.7616095235058475 >50 0.01974684622761815 0.933168316909058 >100 0.009873419446244903 1.1903205719489305 >500 5.641953969281712E-4 0.2619825832758585 >1k 5.641953969281712E-4 1.0952649443885263 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4546 0.8758173460384002 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1123 0.21635347109571568 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 718 0.13832750867918422 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 638 0.12291497289320269 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.926566973247691E-4 0.0 24-25 0.0 0.0 0.0 3.853133946495382E-4 0.0 26-27 0.0 0.0 0.0 7.706267892990764E-4 0.0 28-29 0.0 0.0 0.0 0.002311880367897229 0.0 30-31 0.0 0.0 0.0 0.007995252938977918 0.0 32-33 0.0 0.0 0.0 0.01627949092394299 0.0 34-35 0.0 0.0 0.0 0.02523802734954475 0.0 36-37 0.0 0.0 0.0 0.0411322048788382 0.0 38-39 0.0 0.0 0.0 0.06771882910965633 0.0 40-41 0.0 0.0 0.0 0.10336031811473863 0.0 42-43 0.0 0.0 0.0 0.13293312115409067 0.0 44-45 0.0 0.0 0.0 0.16529944630465188 0.0 46-47 0.0 0.0 0.0 0.20440875586158003 0.0 48-49 0.0 0.0 0.0 0.253247228633409 0.0 50-51 0.0 0.0 0.0 0.3109479094821773 0.0 52-53 0.0 0.0 0.0 0.3648917847331127 0.0 54-55 0.0 0.0 0.0 0.4177760481487618 0.0 56-57 0.0 0.0 0.0 0.4799078330359998 0.0 58-59 0.0 0.0 0.0 0.5484936172836177 0.0 60-61 0.0 0.0 0.0 0.6355744444744132 0.0 62-63 0.0 0.0 0.0 0.725159808730431 0.0 64-65 0.0 0.0 0.0 0.8088691437180431 0.0 66-67 0.0 0.0 0.0 0.9013443584339322 0.0 68-69 0.0 0.0 0.0 1.0104843774684138 0.0 70-71 0.0 0.0 0.0 1.1399496780706588 0.0 72-73 0.0 0.0 0.0 1.2697039637188907 0.0 74-75 0.0 0.0 0.0 1.3999398911104346 0.0 76-77 0.0 0.0 0.0 1.5362445044677089 0.0 78-79 0.0 0.0 0.0 1.6980761302205147 0.0 80-81 0.0 0.0 0.0 1.8943933047944546 0.0 82-83 0.0 0.0 0.0 2.078862092482921 0.0 84-85 0.0 0.0 0.0 2.2721930882483266 0.0 86-87 0.0 0.0 0.0 2.4714001132821384 0.0 88-89 0.0 0.0 0.0 2.7028771351178484 0.0 90-91 0.0 0.0 0.0 2.958339915770492 0.0 92-93 0.0 0.0 0.0 3.223917173032686 0.0 94-95 0.0 0.0 0.0 3.498645623417807 0.0 96-97 0.0 0.0 0.0 3.765956790955924 0.0 98-99 0.0 0.0 0.0 4.053593240061804 0.0 100-101 0.0 0.0 0.0 4.409430160020653 0.0 102-103 0.0 0.0 0.0 4.7942619129268795 0.0 104-105 0.0 0.0 0.0 5.187859545561382 0.0 106-107 0.0 0.0 0.0 5.561806195068759 0.0 108-109 0.0 0.0 0.0 5.951839678802754 0.0 110-111 0.0 0.0 0.0 6.399188529990868 0.0 112-113 0.0 0.0 0.0 6.881697228440752 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1595 0.0 47.507343 1 GGTATCA 915 0.0 39.776485 1 ATCAACG 1795 0.0 38.863804 3 TCAACGC 1795 0.0 38.210537 4 CAACGCA 1840 0.0 37.240055 5 TATTCCG 65 0.0019245282 36.66713 5 AACGCAG 1885 0.0 36.351032 6 TATCAAC 2020 0.0 36.33051 2 GTACATG 3345 0.0 33.35517 1 CGCAGAG 2125 0.0 31.684715 8 TACATGG 3390 0.0 31.504387 2 ACGCAGA 2135 0.0 31.257229 7 TAGGGCG 80 0.0052975635 29.820837 4 ACATGGG 3615 0.0 28.03913 3 GTATAGG 240 4.2018655E-10 27.346334 1 GCGTCGT 55 7.7829184E-4 27.026274 26-27 CGATCGG 45 0.009545845 26.434605 80-81 GAGTACA 2555 0.0 26.154402 1 GCAGAGT 2415 0.0 26.147802 9 AGTACAT 2465 0.0 25.657045 2 >>END_MODULE